Plant Diversity ›› 2014, Vol. 36 ›› Issue (02): 209-218.DOI: 10.7677/ynzwyj201413085

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ISSR and SRAP Markers Reveal Genetic Diversity and Population Structure of an Endangered Slipper Orchid, Paphiopedilum micranthum (Orchidaceae)

 HUANG  Jia-Lin-1、2, LI  Shu-Yun-1, HU  Hong-1   

  1. 1 Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2013-04-11 Online:2014-03-25 Published:2013-09-04
  • Supported by:

    The Basic Research Program of Yunnan Province (2007C001Z) and the LargeScale Scientific Facilities of the Chinese Academy of Sciences (2009LSFGBOWS01)


Paphiopedilum micranthum is an endangered pink slipper orchid mainly distributed in the limestone areas of southwestern China. Wild populations of this species have been seriously threatened by excessive collections, rampant smuggling for export, and habitat destruction. We used 15 ISSR markers and 11 SRAP markers to investigate the genetic diversity and structure of 15 natural populations. A high degree of diversity was observed at the species level (ISSR: PPB=9166%, He=03839; SRAP: PPB=9929%, He=02806). Certain degree of genetic differentiation among populations (ISSR: Gst=02577; SRAP: Gst=02383) was detected maybe caused by low gene flow (ISSR: Nm=07201; SRAP: Nm=07991). Consistent with the results of Principal Coordinate Analysis, the UPGMA dendrogram analysis divided the 15 populations into two main clades. In addition to geographic distance, the difference in elevation was another natural factor contributing to this differentiation. Knowledge about genetic diversity and structure gained from our study will be beneficial for the development of reasonable and efficient strategies to conserve this endangered species.

Key words: Conservation, DNA markers, Genetic diversity, Genetic differentiation, Paphiopedilum micranthum

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