DNA条形码在生态学研究中的应用与展望

doi:10.7677/ynzwyj201313192

摘要/Abstract

摘要：

DNA条形码是利用标准的DNA片段对物种进行快速鉴定的技术，已在生物学各相关领域得到广泛应用。随着DNA条形码技术的不断发展和完善，已成功应用于生态学领域的相关研究中。本文综述了DNA条形码在物种快速鉴定和隐存种发现、群落系统发育重建和生态取证、群落内物种间相互关系研究等方面的应用，并介绍了DNA metabarcoding技术和环境DNA条形码在生物多样性和生态学研究领域中的应用。最后，结合新的测序技术和未来大科学装置的发展，在相关数据库逐渐完善，新分析方法和计算模型不断开发使用的情景下，对DNA条形码在生态学相关领域的应用前景进行了展望。

关键词: 生物多样性, 群落系统发育, DNA metabarcoding, 环境DNA条形码, 第二代测序技术

Abstract:

DNA barcoding is a technique used to identify species, which is now widely employed in different biological disciplines. With the development and modification of DNA barcoding, it has become a useful tool for ecological studies. Here, we review the uses of DNA barcoding for quick species identification and/or new and cryptic species discovery, in community phylogenetic reconstruction and ecological forensics, and in interaction networks among species within a community. The techniques of DNA metabarcoding and environmental DNA metabarcoding used in ecological research such as diet analysis and biodiversity assessment are also examined. Finally, we discuss the future prospects of DNA barcoding in ecological research following the development of nextgeneration sequencing technologies, improvement of Largescale Scientific Facilities, and use of new models and software for data analysis.

Key words: Community phylogeny, Environmental DNA barcoding, DNA metabarcoding, Biodiversity, Next generation sequencing

中图分类号:

Q 948
Q 781

罗亚皇1、2、3、4, 刘杰1, 高连明1**, 李德铢1、2. DNA条形码在生态学研究中的应用与展望[J]. Plant Diversity, 2013, 35(6): 761-768.

LUO Ya-Huang-, LIU Jie-, GAO Lian-Ming-**, LI De-Zhu. Applications and Advances of DNA Barcoding in Ecological Studies[J]. Plant Diversity, 2013, 35(6): 761-768.

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