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Plant Diversity ›› 2011, Vol. 33 ›› Issue (5): 535-539.DOI: 10.3724/SP.J.1143.2011.11004

• 研究论文 • 上一篇    下一篇

濒危植物华木莲核基因组微卫星引物开发研究

 熊敏1, 王静1, 张志荣3, 张志勇1、2   

  1. 1 江西农业大学 亚热带生物多样性实验室,江西 南昌330045; 2 中国科学院植物研究所系统与进化植物学国家
    重点实验室,北京100093;3 中国科学院昆明植物研究所,中国西南野生生物种质资源库,云南 昆明650201
  • 收稿日期:2011-01-07 出版日期:2011-10-25 发布日期:2011-03-03
  • 基金资助:

    江西省自然科学基金项目(2009GZN0016);系统与进化植物学国家重点实验室开放课题;国家自然科学基金(30760016)

The Development of Nuclear Microsatellite Markers for an Endangered Plant, Sinomanglietia glauca (Magnoliaceae)

 XIONG  Min-1, WANG  Jing-1, ZHANG  Zhi-Rong-3, ZHANG  Zhi-Yong-1、2   

  1. 1 Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China; 2 Key State Laboratory
    of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
    3 China Germplasm Bank of Wild Species/Key Laboratory of Biodiversity and Biogeography,
    Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2011-01-07 Online:2011-10-25 Published:2011-03-03
  • Supported by:

    江西省自然科学基金项目(2009GZN0016);系统与进化植物学国家重点实验室开放课题;国家自然科学基金(30760016)

摘要:

华木莲(Sinomanglietia glauca)是在江西宜春和湖南永顺发现的一种珍稀植物。为进一步探讨华木莲群体遗传学特征,本研究采用FIASCO法(fast isolation by AFLP sequences containing repeats)开发了七对华木莲微卫星引物,选取华木莲4个野生居群16个样品对每个位点进行多态性初步检测。结果发现,七个微卫星位点的等位基因数目(NA)为2~7个,观测杂合度(HO)0.079 ~0.989,期望杂合度(HE)0.187~0.600。另外,使用九个华木莲近缘种对七对引物进行通用性检测,发现这些引物在醉香含笑中通用性最低(28.5%),在观光木中通用性最高(85.7%)。本文开发的多态性微卫星标记将用于华木莲的繁育系统、群体遗传学等研究,同时也将用于木兰科其它近缘种的群体遗传学研究。

关键词: 华木莲, 微卫星, 分子标记, 通用性

Abstract:

Sinomanglietia glauca is an endangered species found in Yichun of Jiangxi Province and Yongshun of Hunan Province. To further explore its population genetic characteristics, we developed seven microsatellite markers from S.glauca using a FIASCO (fast isolation by AFLP sequences containing repeats) method. The number of alleles (NA) ranged from two to seven in 16 samples of four wild populations. The ranges of observed (HO) and expected (HE) heterozygosities were 0.079-0.989 and 0.187-0.600, respectively. The transferability of the seven microsatellite loci was tested upon nine related species of S.glauca within Magnoliaceae. Among which, two (28.5%, Michelia macclurei) to six (85.7%, Tsoongiodendron odorum) loci were successfully amplified. These polymorphic microsatellite markers could be used to decipher the genetic structure, breeding system and population history of S.glauca as well as other relatives within Magnoliaceae.

Key words: Sinomanglietia glauca, Microsatellite, Molecular marker, Transferability

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