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Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 761-768.DOI: 10.7677/ynzwyj201313192

• 研究论文 • 上一篇    下一篇


 罗亚皇1、2、3、4, 刘杰1, 高连明1**, 李德铢1、2   

  1. 1 中国科学院昆明植物研究所生物多样性与生物地理学重点实验室,云南 昆明650201;2 中国科学院西南野生生物种质资源库,云南 昆明650201;3 中国科学院大学,北京100049;4 云南大学生命科学学院,云南 昆明650091
  • 收稿日期:2013-09-22 出版日期:2013-11-25 发布日期:2013-09-30
  • 基金资助:

    科技部重大研究计划项目 (2014CB954100);科技基础性工作专项项目 (2012FY110800, 2013FY112600);中国科学院大科学装置项目 (2009-LSFGBOWS-01)

Applications and Advances of DNA Barcoding in Ecological Studies

 LUO  Ya-Huang-1、2、3、4, LIU  Jie-1, GAO  Lian-Ming-1**, LI  De-Zhu-1、2   

  1. 1 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming650201, China; 2 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy
    of Sciences, Kunming 650201, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China;4 School of Life Sciences, Yunnan University, Kunming 650091, China
  • Received:2013-09-22 Online:2013-11-25 Published:2013-09-30
  • Supported by:

    科技部重大研究计划项目 (2014CB954100);科技基础性工作专项项目 (2012FY110800, 2013FY112600);中国科学院大科学装置项目 (2009-LSFGBOWS-01)


DNA条形码是利用标准的DNA片段对物种进行快速鉴定的技术,已在生物学各相关领域得到广泛应用。随着DNA条形码技术的不断发展和完善,已成功应用于生态学领域的相关研究中。本文综述了DNA条形码在物种快速鉴定和隐存种发现、群落系统发育重建和生态取证、群落内物种间相互关系研究等方面的应用,并介绍了DNA metabarcoding技术和环境DNA条形码在生物多样性和生态学研究领域中的应用。最后,结合新的测序技术和未来大科学装置的发展,在相关数据库逐渐完善,新分析方法和计算模型不断开发使用的情景下,对DNA条形码在生态学相关领域的应用前景进行了展望。

关键词: 生物多样性, 群落系统发育, DNA metabarcoding, 环境DNA条形码, 第二代测序技术


DNA barcoding is a technique used to identify species, which is now widely employed in different biological disciplines. With the development and modification of DNA barcoding, it has become a useful tool for ecological studies. Here, we review the uses of DNA barcoding for quick species identification and/or new and cryptic species discovery, in community phylogenetic reconstruction and ecological forensics, and in interaction networks among species within a community. The techniques of DNA metabarcoding and environmental DNA metabarcoding used in ecological research such as diet analysis and biodiversity assessment are also examined. Finally, we discuss the future prospects of DNA barcoding in ecological research following the development of nextgeneration sequencing technologies, improvement of Largescale Scientific Facilities, and use of new models and software for data analysis.

Key words:  Community phylogeny, Environmental DNA barcoding, DNA metabarcoding, Biodiversity, Next generation sequencing