Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments

doi:10.3724/SP.J.1143.2012.12032

Abstract

Abstract:

Several VQ proteins are recently identified as WRKY factorsinteracting partners in Arabidopsis and involved in regulating physiological processes. Searching of genomic databases found that VQ gene family is specific to land plants and these VQ genes encode proteins characteristic of a conserved VQmotif. It consists of 34 representatives in Arabidopsis and can be divided into two groups based on the similarity of the amino acid sequences. To understand the functions of Arabidopsis VQ proteins, we examined the expression profiles of AtVQ genes in various defenserelated hormones treatments. qRTPCR analysis revealed that a majority of them were differently regulated in response to salicylic acid (SA), methyl jasmonate (MeJA), or 1aminocyclopropane1carboxylate (ACC). And some members are induced by two of these three hormones. Moreover, four members (AtVQ3, AtVQ18, AtVQ23 and AtVQ24) are induced by SA, MeJA and ACC simultaneity. However, there is only one AtVQ gene (AtVQ27) is upregulated after spraying of abscisic acid (ABA). These results suggest that Arabidopsis VQ genes may be involved in plant defense responses.

Key words: Arabidopsis thaliana, VQ gene family, ABA, SA, JA, ET

CLC Number:

Q 945

LIN Yuan-Yuan- , YU Di-Qiu. Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments[J]. Plant Diversity, 2012, 34(5): 509-518.

References

Andreasson ET, Jenkins J, Brodersen P et al., 2005. The MAP kinase substrate MKS1 is a regulator of plant defense responses［J］. European Molecular Biology Organization Journal, 24: 2579—2589
Chen LG, Zhang LP, Yu DQ, 2010. Woundinginduced WRKY8 is involved in basal defense in Arabidopsis［J］. Molecular PlantMicrobe Interactions,23 (5): 558—565
Cheng WH, Endo A, Zhou L et al., 2002. A unique shortchain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions［J］. The Plant Cell, 14: 2723—2743
Cheong JJ, Choi YD, 2003. Methyl jasmonate as vital substance in plants［J］. Trends in Genetics,19 (7): 409—413
Clarke JD, Volko SM, Ledford HD et al., 2000. Roles of salicylic acid, jasmonic acid, and ethylene in cprinduced resistance in Arabidopsis［J］. The Plant Cell, 11 (12): 2175—2190
Finkelstein RR, Gampala S, Rock C, 2002. Abscisic acid signaling in seeds and seedlings［J］. The Plant Cell,14(Suppl): S15—S45
Garcia D, Saingery V, Chambrier P et al., 2003. Arabidopsis haiku mutants reveal new controls of seed size by endosperm［J］. Plant Physiology,131 (4): 1661—1670
Genoud T, Trevino Santa Gruz MB, Métraux JP, 2001. Numeric simu
lation of plant signaling networks［J］. Plant Physiology, 126: 1430—1437
Hayat Q, Hayat S, Irfan M et al., 2010. Effect of exogenous salicylic acid under changing environment: A review［J］. Environmental and Experimental Botany, 68 (1): 14—25
Hirayama T, Shinozaki K, 2007. Perception and transduction of abscisic acid signals: keys to the function of the versatile plant hormone ABA［J］. Trends Plant Science, 12 (8): 343—350
Jiang WB, Yu DQ, 2009. Arabidopsis WRKY2 transcription factor mediates seed germination and postgermination arrest of development by abscisic acid［J］. BMC Plant Biology,9 (1): 96
Johnson C, Boden E, Arias J et al., 2003. Salicylic acid and NPR1 induce the recruitment of transactivating TGA factors to a defense gene promoter in Arabidopsis［J］. The Plant Cell, 15 (8): 1846—1858
Kendrick MD, Chang C, 2008. Ethylene signaling: new levels of complexity and regulation［J］. Current Opinion in Plant Biology, 11 (5): 479—485
Lai ZB, Ying L, Chen ZX et al., 2011. Arabidopsis sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense［J］. The Plant Cell, 23 (10): 3824—3841
LeonReyes A, Du YJ, Koorneef A et al., 2010. Ethylene signaling renders the jasmonate response of Arabidopsis insensitive to future suppression by salicylic acid［J］. Molecular PlantMicrobe Interactions, 23 (2): 187—197
LeonReyes A, Spoel SH, De lange ES et al., 2009. Ethylene modulates the role of NONEXPRESSOR OF PATHOGENESISRELATED GENES1 in cross talk between salicylate and jasmonate signaling［J］. Plant Physiology, 149 (4): 1797—1809
Logemann J, Chell JS, Willmitzer L, 1987. Improved method for the isolation of RNA from plant tissues［J］. Analytical Biochemistry, 163 (1): 16—20
LopezMolina L, Mongrand S, Chua NH, 2001. A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis［J］. Proceedings of the National Academy of Sciences of the United States of America, 98 (8): 4782—4787
Luo M, Dennis ES, Berger F et al., 2005. MINISEED3 (MINI3), a WRKY family gene, and HAIKU2 (IKU2), a leucinerich repeat (LRR) KINASE gene, are regulators of seed size in Arabidopsis［J］. Proceedings of the National Academy of Sciences of the United States of America, 102 (48): 17531—17536
Morikawa K, Shiina T, Murakami S et al., 2002. Novel nuclearencoded proteins interacting with a plastid sigma factor, Sig1, in Arabidopsis thaliana［J］. FEBS Letters, 514(23): 300—304
Pandey SP, Somssich IE, 2009. The role of WRKY transcription factors in plant immunity［J］. Plant Physiology, 150 (4): 1648—1655
Penninckx IAMA, Eggermont K, Terras FRG et al., 1996. Pathogeninduced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acidindependent pathway［J］. The Plant Cell, 12 (8): 2309—2323
Perruc E, Charpenteau M, Ramirez BC et al., 2004. A novel calmodulinbinding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings［J］. The Plant Journal, 38 (3): 410—420
Qiu JL, Fiil BK, Petersen K et al., 2008. Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus［J］. European Molecular Biology Organization Journal, 27 (16): 2214—2221
Ross AF, 1961. Systemic acquired resistance induced by localized virus infections in plants［J］. Virology, 14 (3): 340—358
SchroederJl, KwakJM, Allen GJ, 2001. Guard cell abscisic acid signaling and engineering drought hardiness in plants［J］. Nature, 410 (6826): 327—330
Shinshi H, 2008. Ethyleneregulated transcription and crosstalk with jasmonic acid［J］. Plant Science, 175(12): 18—23
Shinozaki K, YamaguchiShinozaki K, Seki M, 2003. Regulatory network of gene expression in the drought and cold stress responses［J］. Current Opinion in Plant Biology, 6 (5): 410—417
Takahashi H, Kanayama Y, Zheng MS et al., 2004. Antagonistic interactions between the SA and JA signaling pathways in Arabidopsis modulate expression of defense genes and geneforgene resistance to cucumber mosaic virus［J］. Plant and Cell Physiology, 45 (6): 803—809
Wang AH, Garcia D et al., 2010. The VQ motif protein IKU1 regulates endosperm growth and seed size in Arabidopsis［J］. The Plant Journal, 63 (4): 670—679
Xie YD, Li W, Guo D et al., 2010. The Arabidopsis gene SIGMA FACTORBINDING PROTEIN 1 plays a role in the salicylate and jasmonatemediated defence responses［J］. Plant Cell and Environment, 33 (5): 828—839
Xu Y, Chang PF, Liu D et al., 1994. Plant defense genes are synergically induced by ethylene and methyl jasmonate［J］. The Plant Cell, 8 (6): 1077—1085附图1SA处理后表达变化不明显的AtVQ成员表达谱

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