Cryopreservation of in vitroGrown Shoot Tips of Red Bud Taro by Encapsulation-Vitrification

doi:10.7677/ynzwyj201515067

Abstract

Abstract:

A simple procedure for cryopreservation of in vitrogrown shoot tips of red bud taro (Colocasia esculenta L. Schott var. cormosus‘Hongyayu’) by encapsulationvitrification is investigated. Shoot tips were excised from 8weekold stock shoots and encapsulated into alginategel beads. Encapsulated shoot tips were precultured in liquid MS medium supplemented with 35mg·L-1 6BA, 05mg·L-1 IBA, 01mg·L-1 GA3 and 03mol·L-1 sucrose for 24h, then they were loaded with a mixture of 2mol·L-1 glycerol plus 04mol·L-1 sucrose for 30min at 25℃. After dehydration with PVS2 at 25℃ for 20min, the encapsulated and dehydrated shoot tips were plunged directly into liquid nitrogen. After rapidly rewarming in a 40℃ water bath for 3min, PVS2 was drained from the cryotubes and replaced third with liquid MS medium supplemented with 35mg·L-1 6BA, 05mg·L-1 IBA, 01mg·L-1 GA3 and 12mol·L-1 sucrose and each kept for 10min at 25℃and then postcultured on solidified MS medium supplemented with 35mg·L-1 6BA, 05mg·L-1 IBA and 01mg·L-1 GA3 in the dark for 3 days and then transferred to the light conditions. The average survival rate amounted to about 80%. Plantlets regenerated from cryopreserved shoot tips were morphologically uniform. This encapsulation vitrification procedure promises to become a routine method for the cryopreservation of shoot tips of Chinese genuine red bud taro.

Key words: Cryopreservation, Encapsulation-vitrification, Red bud taro (Colocasia esculenta var. cormosus‘Hongyayu’), Shoot-tips

CLC Number:

Q 943，S 632.3

WANG Ai-Ping, YIN Ming-Hua, HONG Sen-Rong. Cryopreservation of in vitroGrown Shoot Tips of Red Bud Taro by Encapsulation-Vitrification[J]. Plant Diversity, 2015, 37(06): 801-812.

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