Plant Diversity ›› 2010, Vol. 32 ›› Issue (4): 334-338.DOI: 10.3724/SP.J.1143.2010.10023

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Study on Cryopreservation of Dendrobium chrysanthum (Orchidaceae) Seeds

HE MingGao1,2 , WANG RuiXia3 , SONG XiQiang2 ,  SONG SongQuan3  , ZHANG RuLian1   

  1. 1 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China;
    2 College of Horticulture Science and Landscape Architecture, Hainan University, Danzhou 571737, China;
    3 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;4 Key Laboratory of Protection
    and Developmental Utilization of Tropical Crop Germplasm Resources(Hainan University),
    Ministry of Education, Danzhou 571737, China
  • Received:2010-01-22 Online:2010-08-25 Published:2010-03-15


This work aimed to study the role of plant vitrification solution 2 (PVS2) on seed germination, and the effect of rapid freezing and vitrification treatment on seed cryopreservation of the wild mature seeds of Dendrobium chrysanthum, an endangered orchid species. The results indicated that the seeds germination decreased with increasing treatment time of PVS2. Cryotolerance of seeds was obviously increased by PVS2 pretreatment. When pretreatment time was below 45 min, seeds cryotolerance increased with increasing pretreatment time of PVS2, while it decreased after 60 min. The survival seeds could germinate and develop into normal seedling after storage in liquid nitrogen. In conclusion, mature Dchrysanthum seeds could be successfully cryopreserved after 45 min pretreatment with PVS2.

Key words: Cryopreservation, Dendrobium chrysanthum, Orchid species seeds, Rapid cooling, Vitrification

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