The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana (Fagaceae)

doi:10.7677/ynzwyj201413191

Abstract

Abstract:

Castanopsis tibetana is a typical and dominant component in subtropical evergreen broadleaved forests of China. However, its genetic diversity and longterm persistence have been jeopardized by habitat fragmentation. To examine its genetic diversity and genetic structure, crossspecies amplification of 51 microsatellite loci which are derived from four congeneric species, ie., Castanopsis chinensis, Csclerophylla, Ccuspidata and Csieboldii, were tested in Ctibetana.Thirtyfive loci were successfully amplified in Ctibetana, among them 11 were polymorphic across four wild populations of Ctibetana (24 individuals in total). Genetic diversity of four Ctibetana populations (Jiangxi) was relatively low. The number of alleles (NA) ranged from 2 to 7 (average 45) and the ranges of observed (HO) and expected (HE) heterozygosities were 0505 and 0471，respectively. The results will facilitate the study and conservation of genetic diversity of Ctibetana.

Key words: Castanopsis tibetana, Microsatellite, Transferability, Genetic diversity

CLC Number:

Q 16

YE Lin-Jiang, WANG Jing, SUN Peng, DONG Shu-Peng, ZHANG Zhi-Yong. The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana (Fagaceae)[J]. Plant Diversity, 2014, 36(04): 443-448.

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