Plant Diversity ›› 2014, Vol. 36 ›› Issue (04): 443-448.doi: 10.7677/ynzwyj201413191

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The Transferability of Nuclear Microsatellite Markers in Four Castanopsis Species to Castanopsis tibetana (Fagaceae)

 YE  Lin-Jiang, WANG  Jing, SUN  Peng, DONG  Shu-Peng, ZHANG  Zhi-Yong   

  1. Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2013-09-19 Online:2014-07-25 Published:2014-03-25
  • Supported by:

    国家自然科学基金 (31160043, 31160082);国家科技支撑计划 (2012BAC11B02)

Abstract:

Castanopsis tibetana is a typical and dominant component in subtropical evergreen broadleaved forests of China. However, its genetic diversity and longterm persistence have been jeopardized by habitat fragmentation. To examine its genetic diversity and genetic structure, crossspecies amplification of 51 microsatellite loci which are derived from four congeneric species, ie., Castanopsis chinensis, Csclerophylla, Ccuspidata and Csieboldii, were tested in Ctibetana.Thirtyfive loci were successfully amplified in Ctibetana, among them 11 were polymorphic across four wild populations of Ctibetana (24 individuals in total). Genetic diversity of four Ctibetana populations (Jiangxi)  was relatively low. The number of alleles (NA) ranged from 2 to 7 (average 45) and the ranges of observed (HO) and expected (HE) heterozygosities were 0505 and 0471,respectively. The results will facilitate the study and conservation of genetic diversity of Ctibetana.

Key words: Castanopsis tibetana, Microsatellite, Transferability, Genetic diversity

CLC Number: 

  • Q 16