An Ultrastructural Study of the Spermatogenesis of the Fern Pteris fauriei (Pteridaceae)

doi:10.3724/SP.J.1143.2010.10035

Abstract

Abstract:

The ultrastructural research reveals that the spermatogenesis of the fern Pteris fauriei undergoes the de novo occurrence of the motile organelles, including the blepharoplast, multilayered structure and flagella, the formation of the coiled mitochondrion and nuclear shaping, and finally forms a spiral spermatozoid, which resemble other homosporous ferns described. Some new phenomenon or details during spermatid differentiation in present study including: Spermatids show polar differentiation in the early stage, the nucleus lies in the proximal end (the end of cell plate formation in mitosis), and the blepharoplast, mitochondria and plastids mainly located in the distal end. Abundant microtubules extend from the blepharoplast and plenty of radial mitochondria surround the blepharoplast. The differentiation of the basal bodies undergoes 3 stages, ie. procentriole, centriole and basal body. Their internal structures show different patterns, which are the tubular pattern, cartwheel pattern, and stellate pattern respectively. The amorphous material in the blepharoplast is considered to be the microtubule organizer, from which the multilayered structure, the accessory microtubular ribbon and flagella are differentiated. Abundant membranous structures are produced in the course of spermatid differentiation, which may be used as envelope materials in nuclear shaping. This study reveals some details during spermatogenesis of the fern Pteris fauriei, which contributes to understand the mechanism of spermatogenesis.

Key words: Fern, Pteris fauriei, Spermatogenesis, Ultrastructure

CLC Number:

Q 944

CAO Jian-Guo, DAI Xiao-Fei, WANG Quan-Chi. An Ultrastructural Study of the Spermatogenesis of the Fern Pteris fauriei (Pteridaceae)[J]. Plant Diversity, 2010, 32(4): 303-314.

References

Bell PR, 1974a. The origin of the multilayered structure in the spermatozoid of Pteridium aquilinum\
[J\]. Cytobiologie, 8: 203—212
Bell PR, 1974b. Microtubules in Relation to Flagellogenesis in Pteridium Spermatozoids \
[J\]. Journal of Cell Science, 15: 99—111
Bell PR, and Duckett JG, 1976. Gametogenesis and fertilization in Pteridium \
[J\]. Botanical Journal of the Linnean Society, 73: 47—78
Cao JG, Bao WM, Dai SJ, 2003. Ultrastructure of the Blepharoplast and the Multilayered Structure in Spermatogenesis in Osmunda cinnamomea varasiatica \
[J\]. Acta Botanica Sinica, 45: 832—842
Cao JG (曹建国), Dai XF (代小菲), Dai XL (戴锡玲) et al, 2009. An ultrastructural study of the mature spermatozoid of the fern Pteris fauriei \
[J\]. Journal of Chinese Electron Microscopy Society ( 电子显微学报) , 28 (5): 420—425
CavalierSmith T, 1974. Basal Body and Flagellar Development During the Vegetative Cell Cycle and the Sexual Cycle of Chlamydomonas Reinhardii \
[J\]. Journal of Cell Science, 16: 529—556
Doonan JH, Lloyd C, Duckett JG, 1986. Antitubulin antibodies locate the blapharoplast during spermatogenesis in the fern Platyzoma microphyllum R. BR: A correlated immunofluorescence and electronmicroscopic study \
[J\]. Journal of Cell Science, 81: 243—265
Gori P, Muccifora S, Woo SL et al, 1997. An ultrastructural study of the mature spermatozoid of fern Asplenium trichomanes L. subsp. Trichomanes \
[J\]. Sexual Plant Reproduction, 10: 142—148
Hepler PK, 1976. The blepharoplast of Marsilea: its de novo formation and spindle association \
[J\]. Journal of Cell Science,21: 361—390
Hoffman JC, Vaughn KC, Joshi HC, 1994. Structural and immunocytochemical characterization of microtubule organizing centers in pterdophyte spermatogenous cells \
[J\]. Protoplasma, 179: 46—60
Kotenko JL, 1990. Spermatogenesis in a homosporous fern, Onoclea sensibilis \
[J\]. American Journal of Botany, 77: 809—825
Marc J, Gunning BES, 1986. Immunofluorescent localization of cytoskeletal tubulin and actin during spermatogenesis in Pteridium aquilinum (L.) Kuhn \
[J\]. Protoplasma, 134: 163—177
Muccifora S, Bellani LM, Gori P, 1996. Spermatogenesis in Polystichum setiferum (Forssk.) T. Moore ex Woynar \
[J\]. Giornale Botanico Italiano, 130: 397
Myles DG, Hepler PR, 1977. Spermatogenesis in the fern Marsilea: Microtubules, Nuclear shaping, and Cytomorphogenesis \
[J\]. Journal of Cell Science, 23: 57—86
PickettHeaps JD, 1969. The evolution of the mitotic apparatus: An attempt at comparative ultrastructural cytology in dividing plant cells \
[J\]. Cytobios, 3: 257—280
Renzaglia KS, Garbary DJ, 2001. Motile gametes of land plants: diversity, development, and evolution \
[J\]. Critical Reviews in Plant Sciences, 20: 107—213
Sakaushi S, Okoshi M, Miyamura S et al, 2003. Swimming behavior and ultrastructure of sperm of Lygodium japonicum (Pteridophyta) \
[J\]. Sexual Plant Reproduction, 16: 113—122
Shen J (沈建), Li XG (李新国), Dai XL (戴锡玲) et al, 2009. Development and Apogamy of the Gametophyte of the Fern Pteris fauriei \
[J\]. Bulletin of Botanical Research(植物研究) , 29 (4): 385—390
Vaudois B, Tourte Y, 1979. Spermatogenesis in a pteridophyte 1 first stages of the motile apparatus \
[J\]. Cytobios, 24: 143—156
Vaughn KC, Bowling AJ, 2008. Recovery of microtubules on the blepharoplast of Ceratopteris spermatogenous cells after oryzalin treatment \
[J\]. Protoplasma, 233: 231—240

[1]	Hong Qian, Jian Zhang, Mei-Chen Jiang. Global patterns of fern species diversity: An evaluation of fern data in GBIF [J]. Plant Diversity, 2022, 44(02): 135-140.
[2]	Fa-Guo Wang, Ai-Hua Wang, Cheng-Ke Bai, Dong-Mei Jin, Li-Yun Nie, AJ Harris, Le Che, Juan-Juan Wang, Shi-Yu Li, Lei Xu, Hui Shen, Yu-Feng Gu, Hui Shang, Lei Duan, Xian-Chun Zhang, Hong-Feng Chen, Yue-Hong Yan. Genome size evolution of the extant lycophytes and ferns [J]. Plant Diversity, 2022, 44(02): 141-152.
[3]	Yong-Jiang Huang a, *, Tao Su b, Zhe-Kun Zhou a, b. Late Pliocene diversity and distribution of Drynaria (Polypodiaceae) in western Yunnan explained by forest vegetation and humid climates [J]. Plant Diversity, 2016, 38(04): 194-200.
[4]	SU Bo-, CHANG Yan-Fen. The Exploitation and Utilization of Lycophyte and Fern Resources in Xishuangbanna, Yunnan [J]. Plant Diversity, 2015, 37(06): 856-860.
[5]	ZHOU Xhi-Le, YAN Yue-Hong. Anatomical Study on the Pedicels of Two Ferns [J]. Plant Diversity, 2015, 37(03): 271-274.
[6]	WANG Wan, YANG Wen-Li, DOU Ping, ZHAO Dan-Dan, ZHANG Gang-Min. The Relationship between Aleuritopteris humatifolia and A-chrysophylla Based on Three Chloroplast DNA Sequences Analyses [J]. Plant Diversity, 2015, 37(03): 301-308.
[7]	GUO Yan-Dong, WANG Quan-Xi, LI Bao-Gui, CAO Jian-Guo. Studies on the Development of Gametophyte and Oogenesis of the Fern Microlepia platyphylla [J]. Plant Diversity, 2014, 36(06): 747-754.
[8]	WANG Fan-Hong-, LI De-Zhu-, XUE Chun-Ying-, LEI Jin-Mei. A Comparison of Major Classifications of Chinese Lycophytes and Ferns [J]. Plant Diversity, 2013, 35(6): 791-809.
[9]	LIU Yong, XU Feng-Xia. Pollen Morphology of Four Selected Species in the Annonaceae [J]. Plant Diversity, 2012, 34(5): 443-452.
[10]	ZHANG Peng-Tao^, , HE Juan, XU Gang, TU Lin , GUO Na , ZHAO Yu, PENG Li-Yan , ZHAO Qin-Shi. A New ent-Kauranoid Diterpenoid from Hicriopteris glauca (Gleicheniaceae) [J]. Plant Diversity, 2009, 31(02): 183-186.
[11]	FANG Yun-Shan , YANG Ya-Bing , YANG Ming-Hui , YANG Xue-Qiong , DONG Liu-Hong, DING Zhong-Tao. Chemical Constituents from the Fern Brainea insignis (Blechnaceae) [J]. Plant Diversity, 2008, 30(6): 725-728.
[12]	CAO Jian-Guo, YANG Nai-Ying, WANG Quan-Xi. Observations on the Formation and Ultrastructure of the Egg Membrane in the fern Ceratopteris thalictroides (Parkeriaceae) [J]. Plant Diversity, 2008, 30(05): 543-548.
[13]	GAN Xiao-Hong , DING Yu-Long . A Special Long-Lived Cell: the Culm Fiber of Phyllostachys edulis (Gramineae) [J]. Plant Diversity, 2008, 30(02): 151-158.
[14]	DONG Shi-Yong. The Pteriphyte Flora of Yinggeling Nature Reserve, Hainan Island, China [J]. Plant Diversity, 2007, 29(03): 277-285.
[15]	YANG Feng-Chun, HU Xin-Wen, YOU Li-Li. Geographical Distribution and Floristic Composition of Pteridophytes in Hainan Island [J]. Plant Diversity, 2007, 29(02): 155-160.