The Application of Metabarcoding Technology in Identification of Plant Species Diversity

doi:10.7677/ynzwyj201313198

Abstract

Abstract:

Botany has entered a postFlora era. Benefited from traditional plant taxonomy and related studies, the success of iFlora requires the integration of DNA barcoding through state of art highthroughputsequencing technologies and portable equipment for rapid taxonomic identification and information platform. Traditional plant identification relies mostly on morphological features, while DNA barcoding has been widely applied as a complementary molecular approach to facilitating fast species identification. However, issues remain in classic DNA barcoding, e.g., specieslevel resolution can only be achieved via multiple barcode markers; taxon identity cannot be revealed for pooled plant samples via Sanger sequencing. In this review, the authors introduce recent developments in metabarcoding, which is based on the nextgenerationsequencing technologies, and its applications in a few key aspects in plant diversity and its potential role in iFlora.

Key words: Flora, Plant diversity, DNA barcoding, iFlora, Metabarcoding techonology

CLC Number:

Q 949
Q 78

TANG Min-, YI Ting-Shuang-, WANG Xin-, TAN Mei-Hua-, ZHOU Xin. The Application of Metabarcoding Technology in Identification of Plant Species Diversity[J]. Plant Diversity, 2013, 35(6): 769-773.

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