Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 769-773.doi: 10.7677/ynzwyj201313198

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The Application of Metabarcoding Technology in Identification of Plant Species Diversity

 TANG  Min-1, YI  Ting-Shuang-2, WANG  Xin-1, TAN  Mei-Hua-1, ZHOU  Xin-1   

  1. 1 China National GeneBankShenzhen, BGIShenzhen, Shenzhen 518083, China; 2 Germplasm Bank of Wild Species
    in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2013-09-30 Online:2013-11-25 Published:2013-10-12

Abstract:

Botany has entered a postFlora era. Benefited from traditional plant taxonomy and related studies, the success of iFlora requires the integration of DNA barcoding through state of art highthroughputsequencing technologies and portable equipment for rapid taxonomic identification and information platform. Traditional plant identification relies mostly on morphological features, while DNA barcoding has been widely applied as a complementary molecular approach to facilitating fast species identification. However, issues remain in classic DNA barcoding, e.g., specieslevel resolution can only be achieved via multiple barcode markers; taxon identity cannot be revealed for pooled plant samples via Sanger sequencing. In this review, the authors introduce recent developments in metabarcoding, which is based on the nextgenerationsequencing technologies, and its applications in a few key aspects in plant diversity and its potential role in iFlora.

Key words: Flora, Plant diversity, DNA barcoding, iFlora, Metabarcoding techonology

CLC Number: 

  • Q 949