Natural Hybrids between Ligularia vellerea and L.subspicata (Asteraceae: Senecioneae)

doi:10.7677/ynzwyj201413109

Abstract

Abstract:

Three types of intermediate morphological individuals were found in the sympatric distribution areas of Ligularia vellerea and Lsubspicata. These intermediate individuals were hypothesized to be the hybrids of these two species based on the detailed comparison of their morphological characters. To test this hypothesis, we compared the DNA sequence of Lvellerea, Lsubspicata and the intermediate individuals by using the internal transcribed spacer region of nuclear ribosomal and atpBrbcL intergenic spacer region of the chloroplast. Direct sequencing of ITS clearly had additives and then cloned sequencing cloned out two kinds of sequences that were identical to those putative parental species, thus these putative hybrids were approved. With different morphological characters among three types, their plastid donors were all Lsubspicata according to the maternal sequences of atpBrbcL.

Key words: Ligularia vellerea, L.subspicata, Hybrid progeny, ITS, atpB-rbcL

CLC Number:

Q 321.1
Q 78

YU Jiao-Jun, PAN Li, PAN Yue-Zhi, GONG Xun. Natural Hybrids between Ligularia vellerea and L.subspicata (Asteraceae: Senecioneae)[J]. Plant Diversity, 2014, 36(02): 219-226.

References

Abbott RJ, Hegarty MJ, Hiscock SJ et al., 2010. Homoploid hybrid speciation in action［J］. Taxon, 59 (5): 1375—1386
Abbott RJ, Ritchie MG, Hollingsworth PM, 2008. Introduction. Speciation in plants and animals: pattern and process［J］. Philosophical Transactions of the Royal Society B: Biological Sciences, 363 (1506): 2965—2969
Arnold ML, 1997. Natural Hybridization and Evolution［M］. New York: Oxford University Press
Buerkle CA, Morris RJ, Asmussen MA et al., 2000. The likelihood of homoploid hybrid speciation［J］. Heredity, 84 (4): 441—451
Burland TG, 1999. DNASTAR’s Lasergene sequence analysis software［J］. Methods in Molecular Biology, 132: 71—91
Cao Y, Ma RJ, Wang GX, 2008. The breeding system of three species of genus Ligularia in the east of QinghaiTibet Plateau［J］. Guihaia, 28 (3): 302—306
Chase MW, Paun O, Fay MF, 2010. Hybridization and speciation in angiosperms: a role for pollinator shifts?［J］. BMC Biology, 8 (1): 45
Chiang TY, Schaal BA, Peng CI, 1998. Universal primers for amplification and sequencing a noncoding spacer between the atpB and rbcL genes of chloroplast DNA［J］. Botanical Bulletin of Academia Sinica, 39 : 245—250
CruzMazo G, Buide ML, Samuel R et al., 2009. Molecular phylogeny of Scorzoneroides (Asteraceae): Evolution of heterocarpy and annual habit in unpredictable environments［J］. Molecular Phylogenetics and Evolution, 53 (3): 835—847
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］. Phytochemical Bulletin, 19 (1): 11—15
Gompert Z, Fordyce JA, Forister ML et al., 2006. Homoploid hybrid speciation in an extreme habitat［J］. Science, 314 (5807): 1923—1925
Liu JQ, Liu SW, Ho TN et al., 2001. Karyological studies on the SinoHimalayan genus, Cremanthodiurn (Asteraceae: Senecioneae) ［J］. Botanical Journal of the Linnean Society, 135 (2): 107—112
Liu JQ, Wang YJ, Wang AL et al., 2006. Radiation and diversification within the LigulariaCremanthodiumParasenecio complex (Asteraceae) triggered by uplift of the QinghaiTibetan Plateau［J］. Molecular Phylogenetics and Evolution, 38 (1): 31—49
Liu SW, 1989. CompositaeSenecioneae［A］. In: Ling Y, Liu SW (eds.), Flora Reipublicae Popularis Sinicae［M］. Beijing: Science Press, 4—115
Liu SW, Deng DS, Liu JQ, 1994. Origin, evolution and distribution of Ligularia Cass. (Compositae) ［J］. Acta Phytotaxonomica Sinica, 32 : 514—525
Liu SW, Illarionova ID, 2011. Ligularia［A］. In: Wu ZY, Raven PH, Hong DY (eds.), Flora of China［M］. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press, 371—544
Liu ZJ, 2002. Life History Strategies of Ligularia virgaurea, an Advantage Toxic Forb in Degradative Rangland of Alpine Meadow (Doctor degree) ［D］. Lanzhou: Lanzhou University China
Pan YZ, Shi SH, Gong X et al., 2008. A natural hybrid between Ligularia paradoxa and Lduciformis (Asteraceae, Senecioneae) From Yunnan, China［J］. Annals of the Missouri Botanical Garden, 95 (3): 487—494
Rieseberg LH, 1997. Hybrid origins of plant species［J］. Annual Review of Ecology and Systematics, 28 (1): 359—389
Rieseberg LH, Baird SJE, Gardner KA, 2000. Hybridization, introgression, and linkage evolution［J］. Plant Molecular Biology, 42 (1): 205—224
Rieseberg LH, Raymond O, Rosenthal DM et al., 2003. Major ecological transitions in wild sunflowers facilitated by hybridization［J］. Science, 301 (5637): 1211—1216
Rozas J, SanchezDelBarrio JC, Messeguer X et al., 2003. DnaSP, DNA polymorphism analyses by the coalescent and other methods［J］. Bioinformatics, 19 (18): 2496—2497
Saito Y, Hattori M, Iwamoto Y et al., 2011. Overlapping chemical and genetic diversity in Ligularia lamarum and Ligularia subspicata. Isolation of ten new eremophilanes and a new secobakkane compound［J］. Tetrahedron, 67 (12): 2220—2231
Soltis PS, Soltis DE, 2009. The role of hybridization in plant speciation［J］. Annual Review of Plant Biology, 60: 561—588
Swofford DL, 2002. PAUP*: Phylogenetic Analysis Using Parsimony, Version 40 b10［M］. Sunderland, MA: Sinauer Associates
Thompson JD, Gibson TJ, Plewniak F et al., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools［J］. Nucleic Acids Research, 25 (24): 4876—4882
White TJ, Bruns T, Lee S et al., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics［J］. PCR Protocols: A Guide to Methods and Applications, 18: 315—322
Yu JJ, Kuroda C, Gong X, 2011. Natural hybridization and introgression in sympatric Ligularia species (Asteraceae, Senecioneae) ［J］. Journal of Systematics and Evolution, 49 (5): 438—448
Zhang Q, Liu Y, Sodmergen, 2003. Examination of the cytoplasmic DNA in male reproductive cells to determine the potential for cytoplasmic inheritance in 295 angiosperm species［J］. Plant and Cell Physiology, 44 (9): 941—951
Appendix: List of accessions of Ligularia included in the phylogenetic analysis of nuclear rDNA ITS and atpBrbcL sequences. GenBank numbers for all accessions are provided.
Accessions, GenBank No.
ITS: Ligularia platyglossa, AY458841; Lstenoglossa, AB523365; Ljaponica, AY458825; Latroviolacea, DQ272322; Lthyrsoidea, DQ272320; Ltaquetii, GU323282; Lkongkalingensis, DQ272343; Lcurvisquama, DQ272324; Lcyathiceps, DQ272328; Llatihastata, DQ272334.
atpBrbcL: Lfranchetiana, AB375447; Loligonema, AB523847; Lanoleuca, AB557885; Lveitchiana, AB557887; Ligularia kanaitzensis var. subnudicaulis, AB281495.

[1]	Ming-Yue Jin, Daniel J. Johnson, Guang-Ze Jin, Qing-Xi Guo, Zhi-Li Liu. Soil water content and nitrogen differentially correlate with multidimensional leaf traits of two temperate broadleaf species [J]. Plant Diversity, 2023, 45(06): 694-701.
[2]	Yan Ke, Feng-Ping Zhang, Yun-Bing Zhang, Wei Li, Qin Wang, Da Yang, Jiao-Lin Zhang, Kun-Fang Cao. Convergent relationships between flower economics and hydraulic traits across aquatic and terrestrial herbaceous plants [J]. Plant Diversity, 2023, 45(05): 601-610.
[3]	Amarpreet Kaur, Shalinder Kaur, Harminder Pal Singh, Daizy R. Batish. Is intraspecific trait differentiation in Parthenium hysterophorus a consequence of hereditary factors and/or phenotypic plasticity? [J]. Plant Diversity, 2023, 45(05): 611-620.
[4]	Korina Ocampo-Zuleta, Ángela Parrado-Rosselli. Functional diversity in an Andean subpáramo affected by wildfire in Colombia [J]. Plant Diversity, 2023, 45(04): 385-396.
[5]	Harshita Bhatia, Gaurav Srivastava, R. C. Mehrotra. Legumes from the Paleocene sediments of India and their ecological significance [J]. Plant Diversity, 2023, 45(02): 199-210.
[6]	Chao Liu, Huan-Huan Chen, Li-Zhou Tang, Phyo Kay Khine, Li-Hong Han, Yu Song, Yun-Hong Tan. Plastid genome evolution of a monophyletic group in the subtribe Lauriineae (Laureae, Lauraceae) [J]. Plant Diversity, 2022, 44(04): 377-388.
[7]	Guo-Qiang Zhang, Gui-Zhen Chen, Li-Jun Chen, Jun-Wen Zhai, Jie Huang, Xin-Yi Wu, Ming-He Li, Dong-Hui Peng, Wen-Hui Rao, Zhong-Jian Liu, Si-Ren Lan. Phylogenetic incongruence in Cymbidium orchids [J]. Plant Diversity, 2021, 43(06): 452-461.
[8]	Feng-Ping Zhang, Jiao-Lin Zhang, Timothy J. Brodribb, Hong Hu. Cavitation resistance of peduncle, petiole and stem is correlated with bordered pit dimensions in Magnolia grandiflora [J]. Plant Diversity, 2021, 43(04): 324-330.
[9]	Kai-Wen Jiang, Rong Zhang, Zhong-Fu Zhang, Bo Pan, Bin Tian. DNA barcoding and molecular phylogeny of Dumasia (Fabaceae: Phaseoleae) reveals a cryptic lineage [J]. Plant Diversity, 2020, 42(05): 376-385.
[10]	Ji-Hua Wang, Yan-Fei Cai, Shi-Feng Li, Shi-Bao Zhang. Differences in leaf physiological and morphological traits between Camellia japonica and Camellia reticulata [J]. Plant Diversity, 2020, 42(03): 181-188.
[11]	Ting-Ting Zhang, Na-Yao Zhang, Wei Li, Xiao-Jian Zhou, Xiao-Yu Pei, Yan-Gai Liu, Zhong-Ying Ren, Kun-Lun He, Wen-Sheng Zhang, Ke-Hai Zhou, Fei Zhang, Xiong-Feng Ma, Dai-Gang Yang, Zhong-Hu Li. Genetic structure, gene flow pattern, and association analysis of superior germplasm resources in domesticated upland cotton (Gossypium hirsutum L.) [J]. Plant Diversity, 2020, 42(03): 189-197.
[12]	Wumei Xu, Kyle W. Tomlinson, Jie Li. Strong intraspecific trait variation in a tropical dominant tree species along an elevational gradient [J]. Plant Diversity, 2020, 42(01): 1-6.
[13]	Ashley M. Matheny, Golnazalsadat Mirfenderesgi, Gil Bohrer. Trait-based representation of hydrological functional properties of plants in weather and ecosystem models [J]. Plant Diversity, 2017, 39(01): 1-12.
[14]	HE Wei-Ying-, PAN Yue-Zhi. Study on the DNA Barcoding of Genus Ligularia Cass. (Asteraceae) [J]. Plant Diversity, 2015, 37(06): 693-703.
[15]	JIANG Dai-Feng-, ZHAO Lu-, GUO Hong-, WANG Hai-Ying. New Lineages of Elaphocordyceps Harboring in Lichen Thalli [J]. Plant Diversity, 2015, 37(06): 746-750.