A patatin-like protein synergistically regulated by jasmonate and ethylene signaling pathways plays a negative role in Nicotiana attenuata resistance to Alternaria alternata

doi:10.1016/j.pld.2018.12.001

Plant Diversity ›› 2019, Vol. 41 ›› Issue (01): 7-12.DOI: 10.1016/j.pld.2018.12.001

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A patatin-like protein synergistically regulated by jasmonate and ethylene signaling pathways plays a negative role in Nicotiana attenuata resistance to Alternaria alternata

Junbin Cheng^a,b, Na Song^a,b, Jinsong Wu^a

a Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, 650201, Kunming, China;
b University of Chinese Academy of Sciences, Beijing 10049, China

Received:2018-11-01 Revised:2018-12-24 Online:2019-02-25 Published:2019-03-15
Contact: Jinsong Wu
Supported by:
We thank Prof. Ian T. Baldwin (Max-Planck Institute for Chemical Ecology, Jena, Germany) for providing the seeds of transgenic plants (irACO, Ov-etr1, irAOC and irCOI1), and Biological Technology Open Platform of Kunming Institute of Botany (CAS) for greenhouse and instrument services. This project is supported by the NSFC (Grant No. 31670262), Key Project of Applied Basic Research Program of Yunnan (Grant No. 2014FA040), and 100-Oversea-Top-Talents Recruitment plan of Yunnan to Jinsong Wu.

Abstract

Abstract: Although patatin was initially identified as a major storage protein in potato tubers, patatin-like proteins (PLPs) have been recently reported to be widely present in many plant species and shown to be involved in plant-pathogen interactions. However, it is not clear whether PLPs are involved in Nicotiana attenuata resistance against the necrotrophic fungal pathogen, Alternaria alternata. In this study we identified a NaPLP gene, whose expression was highly elicited by A. alternata inoculation. Silencing NaPLP enhanced N. attenuata resistance to A. alternata, which was associated with higher induction levels of JA and ethylene biosynthetic genes, NaACS1, NaACO1 and NaLOX3. The induction of NaPLP expression by the fungus was abolished in JA-deficient plants and significantly reduced in ethylene-insensitive plants. In addition, NaPLP transcripts were highly induced by exogenous treatment with either methyl jasmonate (MeJA) or ethephon. Co-treatment with MeJA and ethephon led to a much higher induction level of NaPLP transcripts, and this synergistic induction was largely dependent on endogenous JA and ethylene signaling pathways. Thus, we conclude that the NaPLP gene is elicited by A. alternata via JA and ethylene signaling pathways in a synergistic way; however, unlike other JA- and ethylene-induced defense genes, NaPLP negatively affects plant resistance to the fungus likely by suppressing JA and ethylene biosynthetic gene expression.

Key words: Alternaria, Ethylene, Jasmonate, Necrotrophic fungal pathogen, Nicotiana, Plant resistance

Junbin Cheng, Na Song, Jinsong Wu. A patatin-like protein synergistically regulated by jasmonate and ethylene signaling pathways plays a negative role in Nicotiana attenuata resistance to Alternaria alternata[J]. Plant Diversity, 2019, 41(01): 7-12.

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A patatin-like protein synergistically regulated by jasmonate and ethylene signaling pathways plays a negative role in Nicotiana attenuata resistance to Alternaria alternata

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