Phospholipase Dδ is Involved in WoundingInduced Phosphatidic Acid Formation in Arabidopsis

doi:10.7677/ynzwyj201514058

Abstract

Abstract:

Phosphalipase D (PLD) hydrolyzes phospholipids into phosphatidic acid (PA). PLDα1 and δ are the two most abundant members of the 12member PLD family in Arabidopsis. PLDα1 has been demonstrated having role in the woundinginduced PA signalling. However, whether and how PLDδ is involved in woundinginduced PA formation remained unclear. In the present study, the membrane lipids response to wounding was profiled in Wassilewskija (WS) and PLDδ knockout mutant (PLDδKO) of Arabidopsis. The levels of most lipids, including monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylcholine and phosphatidylglycerol had decreased rapidly within 30min after wounding in the two Arabidopsis genotypes. In contrast, the level of PA increased sharply and significantly 30min after wounding. It continued to increase until peaking at 1h postwounding in WS and 3h postwounding in PLDδKO, and then decreased. The PA levels were similar in the two genotypes in untreated leaves and in leaves 6h after wounding. However, these levels were lower in PLDδKO than in WS from 30min to 3h postwounding. The significant difference of PA level between the two genotypes occurred 30min after wounding, when it was about 20% lower in PLDδKO than in WS. These results show that PLDδ is involved in woundinginduced PA formation in Arabidopsis, but its absence induces PA response later and with less intensity than PLDα1.

Key words: Arabidopsis, Wounding, Membrane lipid, PLDδ, Phosphatidic acid

CLC Number:

Q 945

LI Ai-Hua-, LING Li-Zhen-, LI Wei-Qi. Phospholipase Dδ is Involved in WoundingInduced Phosphatidic Acid Formation in Arabidopsis[J]. Plant Diversity, 2015, 37(4): 428-438.

References

Bargmann BO, Laxalt AM, ter Riet B et al., 2009. Multiple PLDs required for high salinity and water deficit tolerance in plants［J］. Plant and Cell Physiology, 50 (1): 78—89
Denich T, Beaudette L, Lee H et al., 2003. Effect of selected environmental and physicochemical factors on bacterial cytoplasmic membranes［J］. Journal of Microbiological Methods, 52 (2): 149—182
Dhondt S, Geoffroy P, Stelmach BA et al., 2000. Soluble phospholipase A2 activity is induced before oxylipin accumulation in tobacco mosaic virusinfected tobacco leaves and is contributed by patatinlike enzymes［J］. The Plant Journal, 23 (4): 431—440
Hanahan DJ, Chaikoff I, 1947. A new phospholipidesplitting enzyme specific for the ester linkage between the nitrogenous base and the phosphoric acid grouping［J］. Journal of Biological Chemistry, 169 (3): 699—705
Jolliffe IT, 2005. Pricipal Component Analysis, 2nd edition［M］. New York: Springer
Katagiri T, Takahashi S, Shinozaki K, 2001. Involvement of a novel Arabidopsis phospholipase D, AtPLDδ, in dehydrationinducible accumulation of phosphatidic acid in stress signalling［J］. The Plant Journal, 26 (6): 595—605
Lee SM, Suh S, Kim S et al., 1997. Systemic elevation of phosphatidic acid and lysophospholipid levels in wounded plants［J］. The Plant Journal, 12 (3): 547—556
Li W, Wang R, Li M et al., 2008. Differential degradation of extraplastidic and plastidic lipids during freezing and postfreezing recovery in Arabidopsis thaliana［J］. Journal of Biological Chemistry, 283 (1): 461—468
Ling L, Li W, Xu Z, 2007. Prifiling the changes of lipid molecular species in response to wounding in Arabidopsis thaliana (Cruciferae) by ESIMS/MS［J］. Acta Botanica Yunnanica (云南植物研究), 29 (6): 671—676
Lojkowska E, 1988. Lipidcomposition and postwounding degradation in potato slices from cultivars differing in susceptibility to autolysis［J］. Potato Research, 31 (3): 541—549
Pappan K, AustinBrown S, Chapman KD et al., 1998. Substrate selectivities and lipid modulation of plant phospholipase Dα,β, andγ［J］. Archives of Biochemistry and Biophysics, 353 (1): 131—140
Pappan K, Qin W, Dyer JH et al., 1997a. Molecular cloning and functional analysis of polyphosphoinositidedependent phospholipase D, PLDβ, from Arabidopsis［J］. Journal of Biological Chemistry, 272 (11): 7055—7061
Pappan K, Zheng S, Wang X, 1997b. Identification and characterization of a novel plant phospholipase D that requires polyphosphoinositides and submicromolar calcium for activity in Arabidopsis［J］. Journal of Biological Chemistry, 272 (11): 7048—7054
Qin W, Pappan K, Wang X, 1997. Molecular heterogeneity of phospholipase D (PLD) cloning of PLDγ and regulation of plant PLDγ,β, andα by polyphosphoinositides and calcium［J］. Journal of Biological Chemistry, 272 (45): 28267—28273
Quartacci MF, Gliic＇ O, Stevanovic＇ B et al., 2002. Plasma membrane lipids in the resurrection plant Ramonda serbica following dehydration and rehydration［J］. Journal of Experimental Botany, 53 (378): 2159—2166
Qu LJ (瞿礼嘉), Gu HY (顾红雅), Bai SN (白书农), translation (original author: Buchanan BB, Gruissem W, Jones RL), 2004. Biochemistry & Molecular Biology of Plant (植物生物化学与分子生物学), Beijing: Science Press
Rawyler A, Pavelic D, Gianinazzi C et al., 1999. Membrane lipid integrity relies on a threshold of ATP production rate in potato cell cultures submitted to anoxia［J］. Plant Physiology, 120 (1): 293—300
Ryu SB, Wang XM, 1996. Activation of phospholipase D and the possible mechanism of activation in woundinduced lipid hydrolysis in castor bean leaves［J］. Biochimica et Biophysica Acta (BBA)Lipids and Lipid Metabolism, 1303 (3): 243—250
Wang C, Zien CA, Afitlhile M et al., 2000. Involvement of phospholipase D in woundinduced accumulation of jasmonic acid in Arabidopsis［J］. The Plant Cell, 12 (11): 2237—2246
Wang X, 1999. The role of phospholipase D in signaling cascades［J］. Plant Physiology, 120 (3): 645—652
Wang X, 2000. Multiple forms of phospholipase D in plants: the gene family, catalytic and regulatory properties, and cellular functions［J］. Progress in Lipid Research, 39 (2): 109—149
Wang X, 2004. Lipid signaling［J］. Current Opinion in Plant Biology, 7 (3): 329—336
Wang X, 2005. Regulatory functions of phospholipase D and phosphatidic acid in plant growth, development, and stress responses［J］. Plant Physiology, 139 (2): 566—573
Wang X, Xu L, Zheng L, 1994. Cloning and expression of phosphatidylcholinehydrolyzing phospholipase D from Ricinus communis L［J］. Journal of Biological Chemistry, 269 (32): 20312—20317
Wasternack C, Stenzel I, Hause B et al., 2006. The wound response in tomatorole of jasmonic acid［J］. Journal of Plant Physiology, 163 (3): 297—306
Welti R, Li W, Li M et al., 2002. Profiling membrane lipids in plant stress responsesRole of phospholipase Dα in freezinginduced lipid changes in Arabidopsis［J］. Journal of Biological Chemistry, 277 (35): 31994—32002
Xu L, Zheng S, Zheng L et al., 1997. Promoter analysis and expression of a phospholipase D gene from castor bean［J］. Plant Physiology, 115 (2): 387—395
Zhang W, Wang C, Qin C et al., 2003. The oleatestimulated phospholipase D, PLDδ, and phosphatidic acid decrease H2O2induced cell death in Arabidopsis［J］. The Plant Cell Online, 15 (10): 2285—2295
Zhang W, Yu L, Zhang Y et al., 2005. Phospholipase D in the signaling networks of plant response to abscisic acid and reactive oxygen species［J］. Biochimica et Biophysica Acta (BBA)Molecular and Cell Biology of Lipids, 1736 (1): 1—9
Zien CA, Wang C, Wang X et al., 2001. In vivo substrates and the contribution of the common phospholipase D, PLDα, to woundinduced metabolism of lipids in Arabidopsis［J］. Biochimica et Biophysica Acta (BBA)Molecular and Cell Biology of Lipids, 1530 (23): 236—248

[1]	Fei-Hong Yan, Li-Ping Zhang, Fang Cheng, Dong-Mei Yu, Jin-Yong Hu. Accession-specific flowering time variation in response to nitrate fluctuation in Arabidopsis thaliana [J]. Plant Diversity, 2021, 43(01): 78-85.
[2]	Mulan Wang a, b, , Yunmei Shen c, , Faqing Tao b, Shengchao Yang a, Weiqi Li b. Submergence induced changes of molecular species in membrane lipids in Arabidopsis thaliana [J]. Plant Diversity, 2016, 38(03): 156-162.
[3]	HAN Xiao-, WANG Hou-Ping-, BO Jin-Jing-, HU Yan-Ru-, CHEN Xiao-Lan-**, YU Di-Qiu. Arabidopsis WRKY8 Transcription FactorAssociated Genes VQ10 and VQ11 are Responsive to Multiple Abiotic Stresses [J]. Plant Diversity, 2015, 37(06): 760-766.
[4]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Changes of Membrane Stability in PotassiumStressed Plants [J]. Plant Diversity, 2014, 36(05): 595-602.
[5]	JIA Yan-Xia, TAO Fa-Qing, LI Wei-Qi. Analysis of Endogenous Hormone Levels to Reveal the Retardation by Suppression of Phospholipase Dα1 in Arabidopsis Leaves during Hormonepromoted Senescence [J]. Plant Diversity, 2014, 36(04): 485-496.
[6]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Plants Adapt to LongTerm Potassium Deficiency by Accumulation of Membrane Lipids in Leaves and Maintenance of Lipid Composition in Roots [J]. Plant Diversity, 2014, 36(02): 163-176.
[7]	YU Xiao-Mei, WANG Rong, LI Wei-Qi. Changes in Membrane Lipids during Silique Senescence in Arabidopsis [J]. Plant Diversity, 2014, 36(02): 177-186.
[8]	YU Xiao-Mei, LI Wei-Qi. The Degree of Unsaturation of Plastidic Membrane Lipids is Positively Associated with Tolerance to Imbibitional Chilling in Soybean Seeds [J]. Plant Diversity, 2014, 36(02): 187-196.
[9]	JIA Yan-Xia-, LI Wei-Qi. Changes in Lysophospholipid and Degree of Unsaturated Membrane Lipids are Associated With Senescence in Arabidopsis Leaves [J]. Plant Diversity, 2013, 35(5): 569-577.
[10]	CHENG Zhi-Peng, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. ST273 Is Essential for Tapetum and Microspore Development in Arabidopsis thaliana* [J]. Plant Diversity, 2012, 34(5): 502-508.
[11]	LIN Yuan-Yuan- , YU Di-Qiu. Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments [J]. Plant Diversity, 2012, 34(5): 509-518.
[12]	ZHENG Guo-Wei-, CHEN Jia-, LI Wei-Qi. Profiling of Membrane Lipids of Arabidopsis Roots during Catechin Treatment [J]. Plant Diversity, 2012, 34(4): 383-.
[13]	YAN Xian-Lun-, WANG Chun-Tao- , KONG Xiang-Xiang-, YANG Yong-Ping-, HU Xiang-Yang. Simplification of Entry Vector by TA Approach [J]. Plant Diversity, 2012, 34(4): 397-.
[14]	LIANG Peng-, KONG Xiang-Xiang-, WANG Chun-Tao-, ZHA Xiang-Dong-, HU Xiang-Yang. Modification of pFGC5941 Construct and Transfer of Double Antisense Genes of NAC1 and SIP1 into Arabidopsis thaliana [J]. Plant Diversity, 2012, 34(4): 403-.
[15]	ZHAO Juan, CHEN Yan, HUI Sheng-Ming, TU Wei-Yi, CHEN Chen, GAO Ju-Fang. Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation [J]. Plant Diversity, 2012, 34(2): 164-170.