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Table of Content
25 April 1991, Volume 13 Issue 02
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PRELIMINARY STUDY ON CALLUS CULTURE OF ONOSMA PANICULATUM
ZHOU Li-Gang, ZHENG Guang-Zhi, XU Chun
1991, 13(02): 1-3.
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2940
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CHEMICAL CONSTITUENTS FROM LIGUSTICUM PTERIDOPHYLLUM
RAO Gao-Xiong, DAI Yun-Hua, WANG Li-Xin, CAI Feng,LIN Zhong-Wen, SUN Han-Dong
1991, 13(02): 1-3.
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2762
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A NOVEL C21 STEROIDAL GLYCOSIDE FROM MARSDENIA INCISA
HEN Ji-Jun,ZHANG Zhuang-Xin,ZHOU Jun,WANG De-Zu,ZHOU Lin,TAO Guo-Da
1991, 13(02): 1-3.
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2307
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Besides a series of normal C21 steroids from Marsdenia incisa incisa P. T. Li et Y.P.Li,a small amount of novel C21 steroidal glycoside(II) had been isolated. The mild acidic hydrolysis of (II) afforded genin-(I).By spectroscopic analyses of IR,EIMS,FDMS,1H NMR,13C NMR (DEPT, PRFT),1H-1H COSY,1H-13CCOSY for(I)and(II),(I)had been deduced as B-nor(7)-6β-formyl-pregnane-3β,5β,8β,12β,14β, 17β,20-heptatol,named neomarinogenin;(II)had been deduced as neomarinogenin 3-O- β-D-thevetopyranosyl- (1→4)- β-D- cymaropyranosyl-(1→4)-β-D-cymaropyranoside,named neomarinoside.Their physical and spectral data were as follows.
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HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF ALKALOIDS IN STEPHANIA PLANTS
RUAN De-Chun, ZHANG Xue-Mei, ZHAO Chun-Jing,WANG Fang-Cai, TIAN Li-Xiao, YANG Chong-Ren
1991, 13(02): 1-3.
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2835
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Composition and contents of eight isoquinolide alkaloids stepholidine(l), rutodine(2), crebaninc( 3 ), isocorydine( 4 ), dicentrine( 5 ), cepharanthine( 6 ), tetrandrine(7) and cycleaninein ( 8 ) in nine species of Stephania plants from yun-nan and Guizhou which were analyzed by high performance liquid chromatogr-aphy (HPLC) on a Zorbax CN column using MeOH : HaO : NH40H as mobile phase and UV (282nm) as detector. The distribution of these alkoloids in the genus of Stephania were discussed.
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THE STRUCTURE OF ESQUIROLIN A
LI Chao-Ming, ZHANG Gui-Hua, LIN Zhong-Wen,ZHENG Hui-Lan, SUN Han-Dong
1991, 13(02): 1-3.
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2426
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A new deterpenoid named as esquirolin A ( 1 ) together with three known compounds, ent-kauran-1β, 17-diol(2), oleanoic acid and β-sitosterol have been isolated from the petroleam ether extract of Coleus esquirolii (Levl) Dunn, by column chromatography on silica gel. The structure of esquirolin A ( 1) was elucidated as ent-17-acctoxy-kauran-16β-ol by spectroscopic methods.
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VARIATIONS IN CHEMICAL COMPONENTS OF LEAF OIL FROM REPRODUCTIVE PROGENIES OF CINNAMOMUM TENUIPILUM
CHENG Bi-Qiang,XU Yong,YU Xue-Jian, DING Jin-Kai, YI Yuan-Fen
1991, 13(02): 1-3.
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2567
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Abstract The chemical components of leaf oil from cutting seedlings through vege-tative propagation of a chemical type of Cinnamomum tenuipilum Kosterm. which is rich in linalool keep the features of the parent, i.e., the content of the main component, linalool, is without obvious variations and other components are al-most the same as those of the parent.
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CHEMICAL CONSTITUENTS OF THE CHINESE DRUG HAN-QIAN-HU (LIGUSTICUM DAUCOIDES)
RAO Gao-Xiong, DAI Yun-Hua, ZHANG Fang, CAI Feng, SUN Han-Dong
1991, 13(02): 1-3.
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2497
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Eleven compounds, two new angular dihydrofuranocoumarins and four known coumarins together with other five known compounds, which have been isolated from the chinese drug Han Qian-Hu (Ligusticum daucoides Franch.).
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THE DITERPENOIDS FROM RABDOSIA DAWOENSIS
ZHAO Qing-Zhi, WANG Qui-Hong, ZHENG Zhi-An,XUE Hua-Zhen, ZHANG Yan-Bing
1991, 13(02): 1-3.
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2847
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A new named dawodensin A (1 ) and a known, xindongnin A (2), dit-erpenoids were isolated from the leaves of Rabdosia dawoensis (Hand.-Mazz.) Hara, Which collected at Xing County, Henan Provice, and their structures were determined by spectroscopic analysis. Dawoensin A is elucidated as ent-lla-hy6roo-xyl-3α, 6β, 7α-triacetoxy-16-Kauren-15-one.
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A PIGMENT OF A FOLK RICE DYEING PLANT BUDDLE1A OFFINALIS
ZOU Cheng, CHEN Si-Ying
1991, 13(02): 1-3.
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2440
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Flowers of Buddleia offinalis(Maxim.)have been used as rice dye for festivals by ethnic groups in southern China since ancient time.It has been reported that the plant can be used for treatment of eye problem. Only the isolation of flavonoids from the plant was reported before, but what was the pigment remained unso-lved.From ethanol extract,we not only isolated apigenin, linarin and D-mannitol,but also determined that the pigment of the plant is crocin by means of spectraldata.
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CHEMICAL AND PHARMACOGNOSTIC STUDIES ON SIPHONOSTEGIA CHINENSIS
HE Zheng-Dan, CAO Yun-Xia, YANG Chong-Ren
1991, 13(02): 1-3.
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2769
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"Ying-Chen" is a famous chinense traditional medicine, which original plant is recorded as Artemisia capillaris Thunb. (Compositae). In the south of China especially Yunnan province, Siphonostegia chinensis Benth., a scrophularious plant, Chinese name as Jin-Zong-Ying-Chen is always used as a substitute of Ying-Chen. Through ancient Chinese medicinal references, this paper describe the utilize history and medicinal value of this plant. In other hand, a chemical study of this plant was carried out. From whole plant of Siphonostegia chinensis, a new iridoidal glucoside together with two known glycosides, 8-epiloganin and acteoside were isolated. The structrue of new compound was elucidated as 10-p-coumaroyl aucubin by means of spectral and chemical methods.
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THE INDUCEMENT, GROWTH OF CALLUS AND ITS PRODUCTION OF α-TOCOPHEROL FROM CARTHAMUS TINCTORIUS
GAN Fan-Yuan, ZHENG Guang-Zhi
1991, 13(02): 1-3.
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2720
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Preliminary identification by TLC and HPLC methods demonstrated that the calli induced from root, hypocotyl, cotyledon and flower bud of Carthamus tinctorius had the capability to synthesize a-tocopherol which has the strongest activity among Vitamin E family. Among these calli, the hypocotyl-callus and its suspension cells were better than others in growth rate and α-tocophcrol content. They reached 0.35, 0.39 gDW/l.d and 0.62, 2.28mg/100gDW respectively. MC medium was rather suitable for the cell growth of the callus which induced from hypocotyl. However, the medium supplemented with 10 CM (Coconut milk) or 0.1% CA(Casamino acid) could obviously raise cell growth rate, reached 0.45% or 0.41%gDW/l.d
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PHOTOSYNTHETIC PROPERTIES OF LEAVES IN VANILLA PLANIFOLIA AMONG DIFFERENT SEASONS
ZHANG Neng-Yi, LI Cun-Xin
1991, 13(02): 1-3.
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3075
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Leaf gas exchange, titratable acidity and chlorophyll content were measured in Vanilla planifolia cultivated in artificial shading condition during four different seasons. The relationship between different leaf ages and photosynthetic types and seasonal variations were researched in detail. The mature leaves perform stable Crassulacean Acid Metabolism (CAM), which uptake and rarely release CO2 for most times during the light period, and express marked diurnal acid fluctuation.
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STUDY ON THE PROPAGATION AND CONSERVATION OF GERMPLASM IN ALSOPHILA SPINULOSA
CHEN Zhi-Ying, ZHANG Feng-Lei, LAN Qing-Ymg,XU Zai-Fu, TAO Guo-Da
1991, 13(02): 1-3.
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3110
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Sporophytes of Alsophila spinulosa were obtained from spore propagation agamospory and adventitious bud produced from callus by tissue culture. The morphology of prothallus is plastic. In the tissue culture, the prothallus had three forms; normal long cordate prothallium, abnormal ligulate prothallium and cylindrical prothallium, and then they could produce normal plant. When BM was a improved MS, the spore germination, filamentous growth and transition from one to two-dimensional growth, prothallium development and sporophyte differentiation etc. were influenced by sugar concentration, inorganic salts concentration, composition and proportion of hormone.
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A PRELIMINARY STUDY ON THE MORPHOGENETIC DEVELOPMENT OF USTILAGO ESCULENTA IN PLANT OF ZIZANIA CADUCIFLORA
SHI Guo-Xin, XU Xiang-Shen
1991, 13(02): 1-3.
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2494
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Mycelium of Ustilago esculenia P. Henn. is perennial which passes the cold winter seasons in rhizome of Zizania caduciflora Handl-Mazz. and invades the shoots developing from buds of the rhizome the next spring. Mycelium is septated, binucleate and profusely branched. Invasion of hyphae is intercellular and intracellular. No appressoria or haustoria are to be found. Cells of the parenchymatous tissue of infected portion of host plant divide rapidly with newly formed cells in great enlargement and high vacuolization which causes the shoot swelling. Part of hyphal cells with their wall transforms into mucous substance at the late stage of shoot swelling. Protoplasm of such mucoid hyphal cells con-tract into round cells, secreting thick wall and change into teliospores (chlamydospores).
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APPLICATION OF GRAPH THEORY IN DIVISION OF FLORISTIC ELEMENTS OF THE GENUS CEPHALOTAXUS IN CHINA
ZUO Jia-Fu
1991, 13(02): 1-3.
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3239
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Based on the theories of Tree and Spanning Tree in Graph theory, this paper selects the data of the distribution of 8 species belonging to the genus Cephalotaxus in China. By means of Graph theoretical methods of classification (that is to say Prim's method in detail), the quantitative study is carried out for the distribution of 8 species belonging to the glenus Cephalotaxus in China. The result shows that this paper is basically successful for the application of Graph theoretical method of classification in the division of the floristic elements of Ce-phalotaxus in China, and the results of this method and the classical analysis are basically unform, especially using the asymmetric information coefficients to con-stitute dissimilarity matrix.
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STUDIES ON THE NET PRIMARY PRODUCTION OF PINUS YUNNANENSIS FOREST
DAN Cheng-Lin, WU Zhao-Lu
1991, 13(02): 1-3.
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2923
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The net primary production of middle-young stands of Pinus Pyunnanensis is not increases with increasing stand age as their biomass. The total amount of the net primary production in the 4-year-old stand is 530.4g/(m2 .a), the 11-year-old stand 1201.0g/(m2.a), the 23-year-old stand 1060.3 g/(m2.a). The percentages of the net production of tree layers in various layers of three stands are the greatest, herb layer second, shrub layer the smallest. The percentage of the net production of tree layer in the 11-year-old stand is the greatest, 23-year-old stand second, 4 -year-old stand the smallest; The percentages of the net production of shrub and herb layers in the 4 -year-old stand are the greatest, 23-year-old stand second, 11-year-old stand the smallest.The net production of needle is the greatest in all organs of tree layers in three stands, stem wood second.
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SPOROCEPHALUM BRYOCOLA, A NEW PARASITIC FUNGUS ON CAPSULES OF MOSSES
ZANG Mu
1991, 13(02): 1-3.
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1776
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Floccis brunneolis,fertilibus simplicibus,apice attenuatis,sporidiis apitato- conglomeratis globosis,brunneolis.Conidiosporae rotundatae,,spinis, 10.4-14.3μm.
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TWO NEW TAXA OF BOLETACEAE FROM FUJIAN
ZANG Mu,HU Mei-Rong,LIU Wo-Peng
1991, 13(02): 1-3.
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2469
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This paper describes two new species of Boletaceae from Fujian Prov. Xerocomus anthracinus Zang, HU et Liu, it shows the blackish basidiocarps different from the other taxa in Genus Xerocomus, it has been found fruiting so-litarily under Casfanopsis hystrix A.DC. and Pinus massoniana Lamb. in Sanming, but the mycorrhizal associations and affinities of this taxon are not known. This mushroom can be easily mistaken for a Boletus when young, but is just as po-isonous. It recently caused several vomiting when after ingestion from Sauming peo-ple. The another new taxon Boletus tomentulosus Zang, Liu et Hu,it is tread here un-der Sect. Squamulistipites, it has the stature and appearance of Boletus squamuli-stipes Zang, but microscopic examination shows the larger basidiospores, 10.4- 14.3 x 5.2-5.5 am and non pleurocystidium, but the basidiospores of latter only 9-11.5 x 4-5.5 am in the meanwhile, the pileus surface has a tendency to become grossly or tomentose in age, not observed in B. squamulistipes specimens.
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A NEW SPECIES OF THE GENUS TRIPOGON (GRAMINEAE) FROM GUIZHOU
PENG Hua
1991, 13(02): 1-3.
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2356
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Affinis T.filiformi Nees ex Steud. Sed laminis supra inter nervos praesertim basi appressis pilis usque ad 10 mm longis; spicis brevioribus,3.5-10 cm longis;spiculis sine bracteolis sub glumis; glumis apice integris acuminatis;lemmatibus brevioribus,tantum tantum 2.5 mm longis,aristis mediis pluries valde reflexis, callo pilisad 0.8 mm longis;paleis lemmatibus leviter breviorbus;stamine 1,anthera ad 1.2 mm longa differt.
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TWO NEW SPECIES OF BAMBOO FROM GUIZHOU
YI Tong-Pei
1991, 13(02): 1-3.
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2057
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Species Ch. marmoreae(Mitford)Makino affinis, sed internodiis culmi brevi-ter tuberculato-hispidis et pubescentibus, radicibus aeriis evolutis,usque ad nods ramosos;vaginis culmi dorso luteolo-tuberculato-setulosis,immaculatids,laminis usque ad 2.8 cm longis; alminis foliorum usque ad 20 cm longis et 2.5 cm latis, nervis secundariis 5-6-jugis,nervillis transversis leviter conspicuis differt.
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A NEW SPECIES OF CAMPANULACEAE FROM EMEISHAN
ZHU Zheng-Yin
1991, 13(02): 1-3.
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1711
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A congenris ceteris differt corollae tubo lobisque intus villosis,discis carenti-bus, filamentis intus pubescentibus.
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A NEW SPECIES OF THE GENUS EURYA FROM YUNNAN
MING Tien-Lu, ZHU Hua
1991, 13(02): 1-3.
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2229
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Affinis E.pittosporifoliae Hu sed gemma terminale pubescente; foliis chartaceis, basi late cuneatis,margine serrulatis non revolutis; sepalis extus puberulis,rmargine ciliatis; staminibus 15-20,antheris non septatis bene differt.
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THE CLASSIFICATION AND DISTRIBUTION OF CHINESE CORYDALIS SECT. ASTEROSTIGMATA
ZHUANG Xuan (H. CHUANG) , WU Zheng-Yi (C. Y. WU)
1991, 13(02): 1-3.
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3056
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Sect. Asterostigmata Fedde is perhaps the most primitive group in genus Corydalis Vent. In this section there are 23 species and 9 varietas and 1 forma all told. They are concentrately distributed in region beyond the south of the Yangtze River of China, where they grow under different kinds of habitat of forest environment below 4000 m, especially in primeval subtropical evergreen bro-ad-leaf forest, a few species extending to East Himalaya, and individual ones even reaching the tropic zone of Southeast Asia on the margin of rain forest. It is divided into 5 series, i. e. ; Ser. 1 . Temulifoliae, Ser. 2 .Schwerinianae, Ser. 3 . Flexuosae, Ser. 4 . Talienses, Ser. 5 . Maireanae.
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ON THE TYPICATION OF TWO SPECIES IN THE GENUS REMUSATIA (ARACEAE)
LI Heng
1991, 13(02): 1-3.
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2577
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The genus Remusatia Schott (Araceae),corrently, includes three species; R. viuipara (Lodd.) Schott, R. hookeriana Schott and R. ornata (Schott) H. Li et Q. F. Guo. 1. Gonatanthus (?) (sic) ornatus Schott should be placed under the genus Remusatia. The specimens collected from Yunnan: S. K. Wu 8637(KUN), R. C. Ching 24875 (KUN, PE, SCBI),D. D. Tao 364(KUN),895(KUN) with bulbi-ferous shoots and bulbils are not different from (7. ornatus, it described by Krause in 1920. After having examined all of the morphological characters of the inflores-cences and much branched bulbiferous shoots of the collection from Yunnan (Q. F. Guo 1103, KUN) we have decided that Gonatanthus(?) ornatus Schott..