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Plant Diversity ›› 2014, Vol. 36 ›› Issue (03): 279-284.DOI: 10.7677/ynzwyj201413135

• 研究论文 • 上一篇    下一篇

基于psbAtrnH序列的十种棘豆属植物分子系统学研究

 卢萍, 高利霞, 金凤, 恩和巴雅尔   

  1. 内蒙古师范大学 生命科学与技术学院,内蒙古 呼和浩特010022
  • 收稿日期:2013-06-17 出版日期:2014-05-25 发布日期:2013-11-09

Molecular Phylogeny of Ten Species of Oxytropis Based on psbAtrnH Sequences

 Lu  Ping, GAO  Li-Xia, JIN  Feng, EN  He-Ba-Ya-Er   

  1. College of Life Science and Technology of Inner Mongolia Normal University, Huhhot 010022, China
  • Received:2013-06-17 Online:2014-05-25 Published:2013-11-09

摘要:

选择内蒙古28个样地采集的10种棘豆属植物56个单株,提取样品的基因组DNA,对其叶绿体psbAtrnH序列进行扩增、测序,所得序列利用ClustalX软件进行对位排列,并用MEGA50软件采用最大似然法构建系统发育树。结果显示:(1)10种棘豆属psbAtrnH序列的变异位点50个,信息位点36个,种间碱基差异百分率为34%,GC含量变化范围在2318%~2572%之间。(2)棘豆属与黄芪属各为一支,自展支持率达99%,这10种棘豆属植物可能为单系起源。(3)系统树中小叶小花棘豆与小花棘豆的样本独立成一支,支持将小叶小花棘豆作为小花棘豆的变种来处理。(4)多叶棘豆、砂珍棘豆和黄毛棘豆的样本相互混杂,表明亲缘关系很近,支持《内蒙古植物志》将三者归入真棘豆亚属轮叶棘豆组的观点。(5)缘毛棘豆与薄叶棘豆的样本聚成一支,支持将二者归入矮生棘豆组。研究表明,psbAtrnH序列可为棘豆属下种间系统发育关系研究提供分子证据。

关键词: 棘豆属, 系统发育, psbA-trnH

Abstract:

Total DNA from 56 individual plants collected in 28 sample sites of 10 Oxytropis species in Inner Mongolia was extracted in this research, and the psbAtrnH sequence was amplied and sequenced. Then the sequences were analyzed by ClustalX and MEGA 50. The phylogenetic trees were constructed by Maximum Likelihood methods in MEGA 50. The results showed as follows: (1) There were 50 variable sites, 36 informative site and 34% of the interspecific difference in the 10 Oxytropis species, and the GC contents of the psbAtrnH region were between 2318%~2572%. (2) The 10 species of Oxytropis were probably monophyletic, clearly separating from the Astragalus species and the bootstrap value was 99%. (3) The samples of Oxytropis glabra var. tenuis and Oglabra clustered a group independently, thus supported the opinion of treating Oxytropis glabra var. tenuis as a variant of Oglabra. (4)The samples of Overticillaris, Oracemosa and Oochrantha mixed. Therefore they showed a close relationship. We are congruent with the points in Flora of Intromongolica, the consensus putting the three species into Subgen. Euoxytropis Sect. Baicalia Stell. ex Bunge. (5) The samples of Ociliata and Oleptophylla clustered a group, which also supported the consensus which put them into sect. Xerobia Bunge. The investigation results indicated that psbAtrnH sequences provided the molecular evidences for the study of the phylogenetic relationships in the Oxytropis at subgenus, sects and species levels.

Key words: Oxytropis, Phylogenetic, psbA-trnH

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