基于psbAtrnH序列的十种棘豆属植物分子系统学研究

doi:10.7677/ynzwyj201413135

摘要/Abstract

摘要：

选择内蒙古28个样地采集的10种棘豆属植物56个单株，提取样品的基因组DNA，对其叶绿体psbAtrnH序列进行扩增、测序，所得序列利用ClustalX软件进行对位排列，并用MEGA50软件采用最大似然法构建系统发育树。结果显示：（1）10种棘豆属psbAtrnH序列的变异位点50个，信息位点36个，种间碱基差异百分率为34%，GC含量变化范围在2318%~2572%之间。（2）棘豆属与黄芪属各为一支，自展支持率达99%，这10种棘豆属植物可能为单系起源。（3）系统树中小叶小花棘豆与小花棘豆的样本独立成一支，支持将小叶小花棘豆作为小花棘豆的变种来处理。（4）多叶棘豆、砂珍棘豆和黄毛棘豆的样本相互混杂，表明亲缘关系很近，支持《内蒙古植物志》将三者归入真棘豆亚属轮叶棘豆组的观点。（5）缘毛棘豆与薄叶棘豆的样本聚成一支，支持将二者归入矮生棘豆组。研究表明，psbAtrnH序列可为棘豆属下种间系统发育关系研究提供分子证据。

关键词: 棘豆属, 系统发育, psbA-trnH

Abstract:

Total DNA from 56 individual plants collected in 28 sample sites of 10 Oxytropis species in Inner Mongolia was extracted in this research, and the psbAtrnH sequence was amplied and sequenced. Then the sequences were analyzed by ClustalX and MEGA 50. The phylogenetic trees were constructed by Maximum Likelihood methods in MEGA 50. The results showed as follows: (1) There were 50 variable sites, 36 informative site and 34% of the interspecific difference in the 10 Oxytropis species, and the GC contents of the psbAtrnH region were between 2318%~2572%. (2) The 10 species of Oxytropis were probably monophyletic, clearly separating from the Astragalus species and the bootstrap value was 99%. (3) The samples of Oxytropis glabra var. tenuis and Oglabra clustered a group independently, thus supported the opinion of treating Oxytropis glabra var. tenuis as a variant of Oglabra. (4)The samples of Overticillaris, Oracemosa and Oochrantha mixed. Therefore they showed a close relationship. We are congruent with the points in Flora of Intromongolica, the consensus putting the three species into Subgen. Euoxytropis Sect. Baicalia Stell. ex Bunge. (5) The samples of Ociliata and Oleptophylla clustered a group, which also supported the consensus which put them into sect. Xerobia Bunge. The investigation results indicated that psbAtrnH sequences provided the molecular evidences for the study of the phylogenetic relationships in the Oxytropis at subgenus, sects and species levels.

Key words: Oxytropis, Phylogenetic, psbA-trnH

中图分类号:

Q 781
Q 949

卢萍, 高利霞, 金凤, 恩和巴雅尔. 基于psbAtrnH序列的十种棘豆属植物分子系统学研究[J]. Plant Diversity, 2014, 36(03): 279-284.

Lu Ping, GAO Li-Xia, JIN Feng, EN He-Ba-Ya-Er. Molecular Phylogeny of Ten Species of Oxytropis Based on psbAtrnH Sequences[J]. Plant Diversity, 2014, 36(03): 279-284.

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