Plant Diversity ›› 2012, Vol. 34 ›› Issue (01): 47-55.DOI: 10.3724/SP.J.1143.2012.11143

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Construction of Leave Library by SSH and Preliminary Analysis of Genes Responsible for Heat Sress in Incarvillea zhongdiannensis

 DING  Yong-1、2, CHANG  Wei-1, ZHANG  Shi-Bao-1, HU  Hong-1   

  1. 1 Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences,
    Kunming 650201, China; 2 School of Life Sciences, Southwest Forestry University, Kunming 650224, China
  • Received:2011-10-17 Online:2012-02-25 Published:2011-12-01


High temperature might be one of the main limiting factors to be introduced and domesticated into lower altitude for Incarvillea zhongdiannensis. To further reveal the underlying molecular mechanism in I.zhongdiannensis responding to high temperature, the suppression subtractive hybridization approach (SSH) was used in this study. The results showed the quality of forward or reverse subtraction cDNA library is good enough for further SSH experiments. Total sixty expressed tags (ESTs) with high quality were randomly obtained by SSH and sequencing. According to BLAST screening and functional annotation, 50 ESTs with known function were classified into 9 broad categories such as defense/stress, photosynthesis, metabolism, energy, signal transduction and transcription, protein fate, transporter, biosynthesis, cell structure and cell growth. There were six ESTs significantly matched with hypothetical proteins. Four ESTs with no hits possibly encode novel genes and play important role in I.zhongdiannensis responding to high temperature stress.

Key words: Incarvillea zhongdiannensis, High temperature stress, Suppression subtractive hybridization (SSH), Expressed sequence tag (EST)

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