Plant Diversity ›› 2013, Vol. 35 ›› Issue (5): 578-584.DOI: 10.7677/ynzwyj201312172

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Genetic Diversity of Rhododendron shanii Based on ISSR Analysis

 ZHAO  Kai-1、2, WANG  De-Yuan-2, ZHANG  Wen-Juan-2, SHAO  Jian-Wen-2   

  1. 1 College of Life Science, Anqing Normal University, Anqing 246011, China; 2 College of
    Life Science, Anhui Normal University, Wuhu 241000, China
  • Received:2012-12-19 Online:2013-09-25 Published:2013-02-25


 Genetic diversity across five sampled populations of Rhododendron shanii Fang was analyzed using ISSR molecular markers. A total of 69 loci, 36 of which were polymorphic, were detected using 8 ISSR primers. At the species level, total percentage of polymorphic loci (P) was 52.17%, and Shannon’s information index (I) and Nei’s gene diversity (h) were 0.2536 and 0.1659, respectively. At the population level, the mean value of P, I and h were 32.46%, 0.1955 and 0.1344, respectively. The coefficient of genetic differentiation (GST) was 0.1845 and the gene flow (Nm) was 2.2104. Analysis of molecular variance (AMOVA) demonstrated that the within populations component accounted for 88.3% of the total variation, while the among populations component accounted for 11.7%. The results of principal component analysis (PCA) and Neighborjoining also revealed that differences among populations were small and most genetic diversity was present within populations. Mantel’s test showed there was significant correlation between genetic and geographical distance. The pattern of genetic structure recorded in R.shanii possibly stems from fairly frequent geneflow among populations, a perennial woody habit and a limited distribution range. At present, priority must be given to in situ conservation of extant populations. In this regard, attention should be paid to large populations of R.shanii, which appear to contain most of the genetic diversity present within the species.

Key words: Rhododendron shanii, Genetic diversity, ISSR, Conservation

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