Plant Diversity ›› 2007, Vol. 29 ›› Issue (01): 98-102.

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Establishment of Genetic Transformation System and Transgenic Studies in Lettuce ( Lactuca sativa var. capatata)

DENG Xiao-Li, ZHOU Yan, CHANG Jing-Ling   

  1. Laboratory of the Mini for Biology, Pingyuan University
  • Received:2006-03-14 Online:2007-02-25 Published:2007-02-25
  • Contact: DENG Xiao-Li

Abstract: In this experiment , the effects of different hormone concentration on shoot regeneration from cotyledon explants are investigated . The results show that MS medium supplied with 1.5 mg/L 6-BA and 0.2 mg/L IAA is the suitable shoot regeneration. The experiment on sensitivity of cotyledon to hygromycin B and carbenicillin shows that the suitable hygromycin concentration for selecting transgenic tissue is 20 mg/L and the carbenicillin concentration is 300 mg/L. Fused gene O21 - O14 - A21 containing both type O and A FMDV is transferred into lettuce mediated by Ti plasmid of Agrobacterium tumafaciens. The results of PCR and PCR-Southern blotting indicated that the O21 - O14 - A21 gene were successfully integrated into the genomes of some transformed lettuce plants. Expression of O21 - O14 - A21gene in vtransformed lettuce plants was proved by RT-PCR analyses .

Key words: FMD epitopes

CLC Number: