Plant Diversity ›› 2011, Vol. 33 ›› Issue (3): 311-329.DOI: 10.3724/SP.J.1143.2011.10191

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An Introduction to Cryopreservation of Plant Germplasm

 WEN  Bin   

  1. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China
  • Received:2010-11-02 Online:2011-06-25 Published:2011-02-10


This paper recited the history of plant germplasm cryopreservation, exhibited the diversity of cryopreservated plant materials, analysed biological foundation of plant cryotolerance, causes and types of cryo-injury, described the key techniques of frequently-used cryopreservation methods, and paid special attention to cryopreservation of plant germplasm of species producing recalcitrant seeds. Based on analysis of potential, status and difficulties, the author thought that regard to cryopreservation of plant germplasm of recalcitrant-seeded species there are much to do, for it was the keystone and difficult part of plant germplasm cryopreservation.

Key words: Germplasm, Cryopreservation, Cryotolerance, Cryo-injury, Recalcitrant seeds

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