Phylogenomic analyses reveal intractable evolutionary history of a temperate bamboo genus (Poaceae: Bambusoideae)

doi:10.1016/j.pld.2019.05.003

Abstract

Abstract: Shibataea is a genus of temperate bamboos (Poaceae:Bambusoideae) endemic to China, but little is known about its phylogenetic position and interspecific relationships. To elucidate the phylogenetic relationship of the bamboo genus Shibataea, we performed genome-scale phylogenetic analysis of all seven species and one variety of the genus using double digest restriction-site associated DNA sequencing (ddRAD-seq) and whole plastid genomes generated using genome skimming. Our phylogenomic analyses based on ddRAD-seq and plastome data congruently recovered Shibataea as monophyletic. The nuclear data resolved S. hispida as the earliest diverged species, followed by S. chinensis, while the rest of Shibataea can be further divided into two clades. However, the plastid and nuclear topologies conflict significantly. By comparing the results of network analysis and topologies reconstructed from different datasets, we identify S. kumasasa as the most admixed species, which may be caused by incomplete lineage sorting (ILS) or interspecific gene flow with four sympatric species. This study highlights the power of ddRAD and plastome data in resolving complex relationships in the intractable bamboo genus.

Key words: Shibataea, ddRAD-seq, Genome skimming, Phylogeny, Incongruence

Cen Guo, Zhen-Hua Guo, De-Zhu Li. Phylogenomic analyses reveal intractable evolutionary history of a temperate bamboo genus (Poaceae: Bambusoideae)[J]. Plant Diversity, 2019, 41(04): 213-219.

Figures/Tables 5

References 34

[1]	Alongi DM, Clough BF, Robertson AI (2005). Nutrient-use efficiency in arid-zone forests of the mangroves Rhizophora stylosa and Avicennia marina.Aquatic Botany, 82, 121-131.
[2]	Ashton PMS, Olander LP, Berlyn GP, Thadani R, Cameron IR (1998). Changes in leaf structure in relation to crown position and tree size of Betula papyrifera within fire-origin stands of interior cedar-hemlock.Canadian Journal of Botany, 76, 1180-1187.
[3]	Berlyn GP, Miksche JP (1976). Botanical Microtechnique and Cytochemistry. The Iowa State University Press, Iowa.
[4]	Carlquist S (2001). Comparative Wood Anatomy. 2nd edn. Springer, Berlin.
[5]	Deng CY, Lin P, Guo SZ (2004a). Study on wood anatomy of Lumnitzera species.Journal of Xiamen University (Natural Science), 43, 406-411. (in Chinese with English abstract)[邓传远, 林鹏, 郭素枝 (2004a). 榄李属(Lumnitzera)红树植物的木材解剖学研究. 厦门大学学报(自然科学版), 43, 406-411. ]
[6]	Deng CY, Lin P, Guo SZ (2004b). Wood structures of some Sonneratia species and their adaptation to intertidal habitats.Acta Phytoecologica Sinica, 28, 392-399. (in Chinese with English abstract)[邓传远, 林鹏, 郭素枝 (2004b). 海桑属红树植物次生木质部解剖特征及其对潮间带生境的适应. 植物生态学报, 28, 392-399. ]
[7]	Fei SL, Fang JY, Fan YJ, Zhao K, Liu XJ, Cui KM (1999). Anatomical characteristics of leaves and woods of Fagus lucida and their relationship to ecological factors in Mountain Fanjingshan, Guizhou, China.Acta Botanica Sinica, 41, 1002-1009. (in Chinese with English abstract)[费松林, 方精云, 樊拥军, 赵坤, 刘雪皎, 崔克明 (1999). 贵州梵净山亮叶水青冈叶片和木材的解剖学特征及其与生态因子的关系. 植物学报, 41, 1002-1009. ]
[8]	Fonti P, von Arx G, García-González I, Eilmann B, Sass- Klaassen U, Gärtner H, Eckstein D (2010). Studying glob- al change through investigation of the plastic responses of xylem anatomy in tree rings.New Phytologist, 185, 42-53.
[9]	Herbette S, Cochard H (2010). Calcium is a major determinant of xylem vulnerability to cavitation.Plant Physiology, 153, 1932-1939.
[10]	IAWA Committee (1989). IAWA list of microscopic features for hardwood identification: With an appendix on non-anatomical information.IAWA Bulletin New Series, 10, 219-332.
[11]	Junghans U, Polle A, Düchtig P, Weiler E, Kuhlman B, Gruber F, Teichmann T (2006). Adaptation to high salinity in poplar involves changes in xylem anatomy and auxin physiology.Plant, Cell & Environment, 29, 1519-1531.
[12]	Kohonen MM, Helland Å (2009). On the function of wall sculpturing in xylem conduits.Journal of Bionic Engineering, 6, 324-329.
[13]	Lin P (1999). Mangrove Ecosystem in China. Science Press, Beijing.
[14]	Liu GS (1996). Soil Physical and Chemical Analysis & Description of Soil Profiles. Standards Press of China, Beijing. (in Chinese)[刘光崧 (1996). 土壤理化分析与剖面描述. 中国标准出版社, 北京. ]
[15]	Lu RK (2000). Analytical Methods for Soil and Agricultural Chemistry. China Agricultural Science and Technology Press, Beijing. (in Chinese)[鲁如坤 (2000). 土壤农业化学分析方法. 中国农业科技出版社, 北京.]
[16]	Nardini A, Lo Gullo MA, Salleo S (2011). Refilling embolized xylem conduits: Is it a matter of phloem unloading?Plant Science, 180, 604-611.
[17]	Plavcová L, Hacke UG (2011). Heterogeneous distribution of pectin epitopes and calcium in different pit types of four angiosperm species.New Phytologist, 192, 885-897.
[18]	Robert EMR, Koedam N, Beeckman H, Schmitz N (2009). A safe hydraulic architecture as wood anatomical explanation for the difference in distribution of the mangroves Avicennia and Rhizophora.Functional Ecology, 23, 649-657.
[19]	Robert EMR, Schmitz N, Okello JA, Boeren I, Beeckman H, Koedam N (2011). Mangrove growth rings: Fact or fiction?Trees, 25, 49-58.
[20]	Salleo S, Trifilò P, Nardini A, Lo Gullo MA (2009). Starch-to- sugar conversion in wood parenchyma of field-growing Laurus nobilis plants: A component of the signal pathway for embolism repair?Functional Plant Biology, 36, 815-825.
[21]	Santandrea G, Schiff S, Bennici A (1998). Effects of manganese on Nicotiana species cultivated in vitro and characterization of regenerated Mn-tolerant tobacco plants.Plant Science, 132, 71-82.
[22]	Schmitz N, Jansen S, Verheyden A, Kairo JG, Beeckman H, Koedam N (2007). Comparative anatomy of intervessel pits in two mangrove species growing along a natural salinity gradient in Gazi Bay, Kenya.Annals of Botany, 100, 271-281.
[23]	Schmitz N, Verheyden A, Beeckman H, Kairo JG, Koedam N (2006). Influence of a salinity gradient on the vessel characters of the mangrove species Rhizophora mucronata.Annals of Botany, 98, 1321-1330.
[24]	Secchi F, Zwieniecki MA (2011). Sensing embolism in xylem vessels: The role of sucrose as a trigger for refilling.Plant, Cell & Environment, 34, 514-524.
[25]	Shi GR, Cheng XL, Liu L, Ma CC (2006). Anatomical and water physiological plasticity of Grewia biloba var. parviflora leaf and secondary xylem.Chinese Journal of Applied Ecology, 17, 1801-1806. (in Chinese with English abstract)[史刚荣, 程雪莲, 刘蕾, 马成仓 (2006). 扁担木叶片和次生木质部解剖和水分生理特征的可塑性. 应用生态学报, 17, 1801-1806. ]
[26]	Shi GR, Liu L (2006). Secondary xylem anatomic characteristics of dominant plant species in three communities in Xiangshan Mountain, Huaibei, China.Acta Botanica Yunnanica, 28, 363-370. (in Chinese with English abstract)[史刚荣, 刘蕾 (2006). 淮北相山三种群落中优势树种次生木质部的解剖学特征. 云南植物研究, 28, 363-370. ]
[27]	Strauss-Debenedetti S, Bazzaz FA (1991). Plasticity and acclimation to light in tropical Moraceae of different sucessional positions.Oecologia, 87, 377-387.
[28]	Sun Q, Lin P (1997). Wood structure of Aegiceras corniculatum and its ecological adaptations to salinities.Hydrobiologia, 352, 61-65.
[29]	Turner NC (1979). Drought resistance and adaptation to water deficits in crop plants. In: Mussell H, Staples RC eds. Stress Physiology in Crop Plants. John Wiley & Sons, New York.
[30]	Tyree MT, Davis SD, Cochard H (1994). Biophysical perspectives of xylem evolution: Is there a tradeoff of hydraulic efficiency for vulnerability to dysfunction?IAWA Journal, 15, 335-360.
[31]	Tyree MT, Zimmermann MH (2002). Xylem Structure and the Ascent of Sap. 2nd edn. Springer, Berlin.
[32]	Verheyden A, de Ridder F, Schmitz N, Beeckman H, Koedam N (2005). High-resolution time series of vessel density in Kenyan mangrove trees reveal a link with climate.New Phytologist, 167, 425-435.
[33]	Yáñez-Espinosa L, Terrazas T, López-Mata L (2001). Effects of flooding on wood and bark anatomy of four species in a mangrove forest community.Trees, 15, 91-97.
[34]	Zwieniecki MA, Holbrook NM. (2009). Confronting Maxwell’s demon: Biophysics of xylem embolism repair.Trends in Plant Science, 14, 530-534.

采样点Sampling sites		土壤中离子含量 Ion content in soil							TSC (g·kg^-1)	pH	TP (g·kg^-1)	OM (g·kg^-1)	TN (g·kg^-1)
采样点Sampling sites		Cl^- (g·kg^-1)	K⁺ (g·kg^-1)	Ca²⁺ (g·kg^-1)	Na⁺ (g·kg^-1)	Mg²⁺ (g·kg^-1)	Al³⁺ (µg·g^-1)	Mn²⁺ (µg·g^-1)	TSC (g·kg^-1)	pH	TP (g·kg^-1)	OM (g·kg^-1)	TN (g·kg^-1)
1	A	4.67 ± 1.15	0.725 ± 0.075	0.15 ± 0.043	4.01 ± 0.13	0.14 ± 0.012	73.59 ± 8.57	557.76 ± 88.64	11.23 ± 1.07	3.02 ± 0.04	0.39 ± 0.04	4.29 ± 0.63	0.75 ± 0.17
	B	4.38 ± 1.51	0.700 ± 0.053	0.16 ± 0.012	3.77 ± 0.09	0.16 ± 0.019	71.82 ± 6.34	504.34 ± 63.24	12.15 ± 1.65	3.50 ± 0.06	0.31 ± 0.04	4.07 ± 0.82	0.88 ± 0.19
	C	4.21 ± 1.26	0.752 ± 0.082	0.18 ± 0.029	4.23 ± 0.19	0.13 ± 0.023	66.57 ± 8.91	556.32 ± 75.52	10.12 ± 2.01	4.01 ± 0.07	0.48 ± 0.06	5.40 ± 0.67	0.95 ± 0.12
2	A	5.36 ± 1.11	0.843 ± 0.098	0.35 ± 0.015	4.10 ± 0.23	0.17 ± 0.025	63.24 ± 10.02	499.72 ± 101.22	11.49 ± 1.32	7.24 ± 0.05	1.15 ± 0.06	8.42 ± 0.89	1.54 ± 0.37
	B	5.42 ± 1.21	0.792 ± 0.029	0.29 ± 0.019	3.27 ± 0.15	0.12 ± 0.014	64.25 ± 9.58	510.07 ± 97.25	11.81 ± 1.43	6.92 ± 0.04	1.14 ± 0.09	8.31 ± 0.48	1.49 ± 0.31
	C	4.85 ± 1.03	0.834 ± 0.092	0.27 ± 0.021	3.73 ± 0.11	0.11 ± 0.010	58.61 ± 9.12	505.36 ± 107.45	11.49 ± 1.52	7.65 ± 0.11	1.11 ± 0.06	7.72 ± 0.64	1.26 ± 0.28
3	A	6.87 ± 1.06	0.670 ± 0.095	0.31 ± 0.012	4.34 ± 0.09	0.15 ± 0.022	49.87 ± 6.33	621.67 ± 94.68	12.36 ± 1.09	7.12 ± 0.08	1.16 ± 0.10	7.68 ± 0.73	1.56 ± 0.43
	B	6.98 ± 0.96	0.540 ± 0.084	0.37 ± 0.024	4.17 ± 0.22	0.25 ± 0.017	54.55 ± 7.45	687.69 ± 99.32	13.86 ± 1.17	6.72 ± 0.08	1.05 ± 0.09	7.31 ± 0.82	1.72 ± 0.32
	C	6.52 ± 1.24	0.610 ± 0.076	0.33 ± 0.019	4.05 ± 0.16	0.16 ± 0.021	47.41 ± 4.13	630.21 ± 103.25	14.37 ± 1.58	7.02 ± 0.07	0.91 ± 0.08	7.17 ± 0.77	1.11 ± 0.24
4	A	7.87 ± 1.32	0.727 ± 0.085	0.42 ± 0.016	4.68 ± 0.24	0.12 ± 0.022	43.54 ± 8.41	505.61 ± 75.65	17.75 ± 1.35	6.94 ± 0.14	0.86 ± 0.05	6.75 ± 0.69	0.59 ± 0.21
	B	7.85 ± 1.22	0.746 ± 0.046	0.49 ± 0.025	4.44 ± 0.29	0.10 ± 0.018	38.81 ± 7.59	545.32 ± 98.64	16.36 ± 1.87	6.05 ± 0.09	0.77 ± 0.02	7.50 ± 0.72	0.49 ± 0.19
	C	6.94 ± 1.19	0.790 ± 0.091	0.45 ± 0.028	4.95 ± 0.13	0.13 ± 0.029	41.21 ± 5.39	571.88 ± 82.63	19.38 ± 1.96	6.79 ± 0.03	0.91 ± 0.03	6.21 ± 0.77	0.65 ± 0.28
5	A	8.63 ± 0.98	0.521 ± 0.102	0.34 ± 0.014	3.41 ± 0.12	0.15 ± 0.031	44.74 ± 11.25	569.21 ± 97.54	12.70 ± 1.23	6.85 ± 0.07	1.06 ± 0.14	6.69 ± 0.34	1.27 ± 0.41
	B	8.31 ± 1.09	0.485 ± 0.079	0.37 ± 0.015	3.77 ± 0.17	0.15 ± 0.027	37.22 ± 7.73	528.96 ± 102.57	12.12 ± 1.54	5.64 ± 0.04	1.25 ± 0.13	5.70 ± 0.45	1.12 ± 0.26
	C	9.23 ± 1.13	0.519 ± 0.099	0.33 ± 0.021	4.28 ± 0.14	0.12 ± 0.022	35.98 ± 10.36	584.21 ± 85.95	13.23 ± 2.01	6.64 ± 0.06	1.30 ± 0.21	6.69 ± 0.38	1.13 ± 0.33
6	A	10.95 ± 0.97	0.347 ± 0.086	0.06 ± 0.011	5.16 ± 0.15	0.07 ± 0.013	28.93 ± 6.12	129.43 ± 64.57	14.86 ± 1.82	6.87 ± 0.10	0.30 ± 0.04	9.39 ± 0.66	1.20 ± 0.25
	B	10.36 ± 1.13	0.358 ± 0.056	0.08 ± 0.016	5.90 ± 0.26	0.09 ± 0.014	33.65 ± 3.25	102.57 ± 81.47	15.42 ± 1.52	6.53 ± 0.05	0.37 ± 0.02	10.80 ± 0.47	1.48 ± 0.31
	C	12.24 ± 1.28	0.301 ± 0.092	0.05 ± 0.013	5.81 ± 0.32	0.05 ± 0.011	26.35 ± 4.99	122.33 ± 57.39	14.52 ± 1.69	6.93 ± 0.06	0.27 ± 0.04	10.20 ± 0.52	1.34 ± 0.23

采样点Sampling sites		土壤中离子含量 Ion content in soil							TSC (g·kg^-1)	pH	TP (g·kg^-1)	OM (g·kg^-1)	TN (g·kg^-1)
采样点Sampling sites		Cl^- (g·kg^-1)	K⁺ (g·kg^-1)	Ca²⁺ (g·kg^-1)	Na⁺ (g·kg^-1)	Mg²⁺ (g·kg^-1)	Al³⁺ (µg·g^-1)	Mn²⁺ (µg·g^-1)	TSC (g·kg^-1)	pH	TP (g·kg^-1)	OM (g·kg^-1)	TN (g·kg^-1)
1	A	4.67 ± 1.15	0.725 ± 0.075	0.15 ± 0.043	4.01 ± 0.13	0.14 ± 0.012	73.59 ± 8.57	557.76 ± 88.64	11.23 ± 1.07	3.02 ± 0.04	0.39 ± 0.04	4.29 ± 0.63	0.75 ± 0.17
	B	4.38 ± 1.51	0.700 ± 0.053	0.16 ± 0.012	3.77 ± 0.09	0.16 ± 0.019	71.82 ± 6.34	504.34 ± 63.24	12.15 ± 1.65	3.50 ± 0.06	0.31 ± 0.04	4.07 ± 0.82	0.88 ± 0.19
	C	4.21 ± 1.26	0.752 ± 0.082	0.18 ± 0.029	4.23 ± 0.19	0.13 ± 0.023	66.57 ± 8.91	556.32 ± 75.52	10.12 ± 2.01	4.01 ± 0.07	0.48 ± 0.06	5.40 ± 0.67	0.95 ± 0.12
2	A	5.36 ± 1.11	0.843 ± 0.098	0.35 ± 0.015	4.10 ± 0.23	0.17 ± 0.025	63.24 ± 10.02	499.72 ± 101.22	11.49 ± 1.32	7.24 ± 0.05	1.15 ± 0.06	8.42 ± 0.89	1.54 ± 0.37
	B	5.42 ± 1.21	0.792 ± 0.029	0.29 ± 0.019	3.27 ± 0.15	0.12 ± 0.014	64.25 ± 9.58	510.07 ± 97.25	11.81 ± 1.43	6.92 ± 0.04	1.14 ± 0.09	8.31 ± 0.48	1.49 ± 0.31
	C	4.85 ± 1.03	0.834 ± 0.092	0.27 ± 0.021	3.73 ± 0.11	0.11 ± 0.010	58.61 ± 9.12	505.36 ± 107.45	11.49 ± 1.52	7.65 ± 0.11	1.11 ± 0.06	7.72 ± 0.64	1.26 ± 0.28
3	A	6.87 ± 1.06	0.670 ± 0.095	0.31 ± 0.012	4.34 ± 0.09	0.15 ± 0.022	49.87 ± 6.33	621.67 ± 94.68	12.36 ± 1.09	7.12 ± 0.08	1.16 ± 0.10	7.68 ± 0.73	1.56 ± 0.43
	B	6.98 ± 0.96	0.540 ± 0.084	0.37 ± 0.024	4.17 ± 0.22	0.25 ± 0.017	54.55 ± 7.45	687.69 ± 99.32	13.86 ± 1.17	6.72 ± 0.08	1.05 ± 0.09	7.31 ± 0.82	1.72 ± 0.32
	C	6.52 ± 1.24	0.610 ± 0.076	0.33 ± 0.019	4.05 ± 0.16	0.16 ± 0.021	47.41 ± 4.13	630.21 ± 103.25	14.37 ± 1.58	7.02 ± 0.07	0.91 ± 0.08	7.17 ± 0.77	1.11 ± 0.24
4	A	7.87 ± 1.32	0.727 ± 0.085	0.42 ± 0.016	4.68 ± 0.24	0.12 ± 0.022	43.54 ± 8.41	505.61 ± 75.65	17.75 ± 1.35	6.94 ± 0.14	0.86 ± 0.05	6.75 ± 0.69	0.59 ± 0.21
	B	7.85 ± 1.22	0.746 ± 0.046	0.49 ± 0.025	4.44 ± 0.29	0.10 ± 0.018	38.81 ± 7.59	545.32 ± 98.64	16.36 ± 1.87	6.05 ± 0.09	0.77 ± 0.02	7.50 ± 0.72	0.49 ± 0.19
	C	6.94 ± 1.19	0.790 ± 0.091	0.45 ± 0.028	4.95 ± 0.13	0.13 ± 0.029	41.21 ± 5.39	571.88 ± 82.63	19.38 ± 1.96	6.79 ± 0.03	0.91 ± 0.03	6.21 ± 0.77	0.65 ± 0.28
5	A	8.63 ± 0.98	0.521 ± 0.102	0.34 ± 0.014	3.41 ± 0.12	0.15 ± 0.031	44.74 ± 11.25	569.21 ± 97.54	12.70 ± 1.23	6.85 ± 0.07	1.06 ± 0.14	6.69 ± 0.34	1.27 ± 0.41
	B	8.31 ± 1.09	0.485 ± 0.079	0.37 ± 0.015	3.77 ± 0.17	0.15 ± 0.027	37.22 ± 7.73	528.96 ± 102.57	12.12 ± 1.54	5.64 ± 0.04	1.25 ± 0.13	5.70 ± 0.45	1.12 ± 0.26
	C	9.23 ± 1.13	0.519 ± 0.099	0.33 ± 0.021	4.28 ± 0.14	0.12 ± 0.022	35.98 ± 10.36	584.21 ± 85.95	13.23 ± 2.01	6.64 ± 0.06	1.30 ± 0.21	6.69 ± 0.38	1.13 ± 0.33
6	A	10.95 ± 0.97	0.347 ± 0.086	0.06 ± 0.011	5.16 ± 0.15	0.07 ± 0.013	28.93 ± 6.12	129.43 ± 64.57	14.86 ± 1.82	6.87 ± 0.10	0.30 ± 0.04	9.39 ± 0.66	1.20 ± 0.25
	B	10.36 ± 1.13	0.358 ± 0.056	0.08 ± 0.016	5.90 ± 0.26	0.09 ± 0.014	33.65 ± 3.25	102.57 ± 81.47	15.42 ± 1.52	6.53 ± 0.05	0.37 ± 0.02	10.80 ± 0.47	1.48 ± 0.31
	C	12.24 ± 1.28	0.301 ± 0.092	0.05 ± 0.013	5.81 ± 0.32	0.05 ± 0.011	26.35 ± 4.99	122.33 ± 57.39	14.52 ± 1.69	6.93 ± 0.06	0.27 ± 0.04	10.20 ± 0.52	1.34 ± 0.23

解剖性状 Anatomical characteristics	生态因子 Ecological factor	回归方程 Regression equation	校正决定系数Adjusted R²
VEL	土壤全盐含量 Total soil salt content	Y _VEL = 344.761 + 12.039X_TSC	0.386^**
RD	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content	Y _RD= 101.890 - 0.042X _Mn2+- 5.777X_Na+	0.726^**
RD50	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content	Y_RD= 128.047 - 0.050X _Mn2+- 5.634X_Na+	0.587^**
TD	土壤全磷含量 Soil total phosphorus content、土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content、土壤有机质含量 Soil organic matter content、土壤Cl-含量 Soil Cl^-content	Y_TD= 71.773 + 12.836 X_TP- 0.033X _Mn2+- 3.589X_Na+- 0.901X_{OM +} 0.547 X_Cl-	0.896^**
PA	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content、土壤全磷含量 Soil total phosphorus content、土壤有机质含量 Soil organic matter content	Y_PA= 5262.683 - 3.836 X _Mn2+ + 916.810X_TP-315.615X_Na+ -75.814X_OM	0.900^**
PA50	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content、土壤全磷含量 Soil total phosphorus content、土壤有机质含量 Soil organic matter content	Y_PA50= 8978.891 - 6.142X_Mn2+- 454.463X_Na++ 1191.560X_TP- 177.127X_OM	0.815^**
CA	土壤pH值 Soil pH value	Y_CA= 5.695 + 0.453X_pH	0.385^**
PD	土壤Ca²⁺含量、土壤全盐量 Total soil salt content	Y_PD= 10.615 + 40.737X_Ca2++ 1.608X_TSC	0.762^**
RH	土壤Ca²⁺含量 Soil Ca²⁺ content	Y_RH= 967.204 + 754.122X_Ca2+	0.422^**
RW	土壤Ca²⁺含量 Soil Ca²⁺ content、土壤全磷含量 Soil total phosphorus content、土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content	Y_RW= 24.786 + 20.811X_Ca2+- 8.091X_TP+ 0.024X _Mn2++ 3.194X_Na+	0.723^**
RF	土壤全磷含量 Soil total phosphorus content、土壤Ca²⁺含量 Soil Ca²⁺ content	Y_RF= 9.331 - 2.759X_TP + 6.038X_Ca2+	0.562^**
FL	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Ca²⁺含量 Soil Ca²⁺ content	Y_FL = 1287.519 - 0.687X_Mn2+ + 491.239X_Ca2+	0.819^**
FWT	土壤Mn²⁺含量 Soil Mn²⁺ content	Y_FWT= 6.868 - 0.002X	0.441^**

解剖性状 Anatomical characteristics	生态因子 Ecological factor	回归方程 Regression equation	校正决定系数Adjusted R²
VEL	土壤全盐含量 Total soil salt content	Y _VEL = 344.761 + 12.039X_TSC	0.386^**
RD	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content	Y _RD= 101.890 - 0.042X _Mn2+- 5.777X_Na+	0.726^**
RD50	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content	Y_RD= 128.047 - 0.050X _Mn2+- 5.634X_Na+	0.587^**
TD	土壤全磷含量 Soil total phosphorus content、土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content、土壤有机质含量 Soil organic matter content、土壤Cl-含量 Soil Cl^-content	Y_TD= 71.773 + 12.836 X_TP- 0.033X _Mn2+- 3.589X_Na+- 0.901X_{OM +} 0.547 X_Cl-	0.896^**
PA	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content、土壤全磷含量 Soil total phosphorus content、土壤有机质含量 Soil organic matter content	Y_PA= 5262.683 - 3.836 X _Mn2+ + 916.810X_TP-315.615X_Na+ -75.814X_OM	0.900^**
PA50	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content、土壤全磷含量 Soil total phosphorus content、土壤有机质含量 Soil organic matter content	Y_PA50= 8978.891 - 6.142X_Mn2+- 454.463X_Na++ 1191.560X_TP- 177.127X_OM	0.815^**
CA	土壤pH值 Soil pH value	Y_CA= 5.695 + 0.453X_pH	0.385^**
PD	土壤Ca²⁺含量、土壤全盐量 Total soil salt content	Y_PD= 10.615 + 40.737X_Ca2++ 1.608X_TSC	0.762^**
RH	土壤Ca²⁺含量 Soil Ca²⁺ content	Y_RH= 967.204 + 754.122X_Ca2+	0.422^**
RW	土壤Ca²⁺含量 Soil Ca²⁺ content、土壤全磷含量 Soil total phosphorus content、土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Na⁺含量 Soil Na⁺ content	Y_RW= 24.786 + 20.811X_Ca2+- 8.091X_TP+ 0.024X _Mn2++ 3.194X_Na+	0.723^**
RF	土壤全磷含量 Soil total phosphorus content、土壤Ca²⁺含量 Soil Ca²⁺ content	Y_RF= 9.331 - 2.759X_TP + 6.038X_Ca2+	0.562^**
FL	土壤Mn²⁺含量 Soil Mn²⁺ content、土壤Ca²⁺含量 Soil Ca²⁺ content	Y_FL = 1287.519 - 0.687X_Mn2+ + 491.239X_Ca2+	0.819^**
FWT	土壤Mn²⁺含量 Soil Mn²⁺ content	Y_FWT= 6.868 - 0.002X	0.441^**