Construction of Leave Library by SSH and Preliminary Analysis of Genes Responsible for Heat Sress in Incarvillea zhongdiannensis

doi:10.3724/SP.J.1143.2012.11143

Abstract

Abstract:

High temperature might be one of the main limiting factors to be introduced and domesticated into lower altitude for Incarvillea zhongdiannensis. To further reveal the underlying molecular mechanism in I.zhongdiannensis responding to high temperature, the suppression subtractive hybridization approach (SSH) was used in this study. The results showed the quality of forward or reverse subtraction cDNA library is good enough for further SSH experiments. Total sixty expressed tags (ESTs) with high quality were randomly obtained by SSH and sequencing. According to BLAST screening and functional annotation, 50 ESTs with known function were classified into 9 broad categories such as defense/stress, photosynthesis, metabolism, energy, signal transduction and transcription, protein fate, transporter, biosynthesis, cell structure and cell growth. There were six ESTs significantly matched with hypothetical proteins. Four ESTs with no hits possibly encode novel genes and play important role in I.zhongdiannensis responding to high temperature stress.

Key words: Incarvillea zhongdiannensis, High temperature stress, Suppression subtractive hybridization (SSH), Expressed sequence tag (EST)

CLC Number:

Q 78，Q 945

DING Yong-, CHANG Wei-, ZHANG Shi-Bao-, HU Hong. Construction of Leave Library by SSH and Preliminary Analysis of Genes Responsible for Heat Sress in Incarvillea zhongdiannensis[J]. Plant Diversity, 2012, 34(01): 47-55.

References

王文采, 潘开玉, 张志耘等, 1990. 中国植物志第69卷［M］. 北京：科技出版社, 44—45
Bernacchi CJ, Portis AR, Nakano H et al., 2002. Temperature response of mesophyll conductance. Implications for the determination of Rubisco enzyme kinetics and for limitations to photosynthesis in vivo［J］. Plant Physiology, 130: 1992—1998
Buchner J, 1999. Hsp90 & Co.-a holding for folding［J］. Trends in Biochemical Sciences, 24: 136—141
Cai YF (蔡艳飞), Zhang SB (张石宝), Hu H (胡虹) et al., 2008. The response of Incarvillea delavayi (Bignoniaceae) grown in different altitudes subject to water availability［J］. Acta Botanica Yunnanica (云南植物研究), 30: 577—585
CraftsBrandner SJ, Salvucci ME, 2000. Rubisco activase constrains the photosynthetic potential of leaves at high temperature and CO2［J］. Proceedings of the National Academy of Sciences of the USA, 97: 13430—13435
Diatachenko L, Lau YFC, Campbell AP et al., 1996. Suppression subtractive hybridization: A method for generating differentially regulated or tissuespecific cDNA probes and libraries［J］. Proceedings of the National Academy of Sciences of the USA, 93: 6025—6030
Du YX (杜有新), Yang T (杨涛), Li CC (李成才) et al., 2003. Investigation of alpine wild ornamental plants germplasm resources in Yunnan province［J］. Journal of Nanjing Forestry University (Natural Sciences Edition) (南京林业大学学报 (自然科学版)), 27: 80—84
Feng GM (冯国楣), 1981. The richness of the ornamental flora in Yunnan［J］. Acta Horticulturae Sinica (园艺学报), 8: 59—64
Frydman J, 2001. Folding of newly translated proteins in vivo: the role of molecular chaperones［J］. Annual Review of Biochemistry, 70: 603—647
Guy C, 1999. The influence of temperature extremes on gene expression, genomic structure, and the evolution of induced tolerance in plants［A］. In: Lerner HR, ed. Plant Responses to Environmental Stresses［M］. New York, NY: Marcel Dekker, 497—548
Hartl FU, 1996. Molecular chaperones in cellular protein folding［J］. Nature, 381: 571—580
Harvey PJ, Campanella BF, Castro PML et al., 2002. Phytoremediation of polyaromatic hydrocarbons, anilines and phenols［J］. Enviromental Science and Pollution Research international, 9: 29—47
Huang JW (黄锦文), Wu WX (吴文祥), Chen DM (陈冬梅) et al., 2009. Analysis of different expression of genes in Zoysia Matrella exposed to high temperature treatmen［J］. Acta Agrestia Sinica (草地学报), 17: 435—439
Karlin S, Brocchieri L, 1998. Heat shock protein 70 family: multiple sequence comparisons, function, and evolution［J］. Journal of Molecular Evolution, 47: 565—577
Lei M (雷鸣), Li SY (李树云), Zhang SB (张石宝) et al., 2009. Effects of nitrogen on photosynthesis and growth in Incarvillea delavayi (Bignoniaceae) ［J］. Acta Botanica Yunnanica (云南植物研究), 31: 82—88
Mandhania S, Madan S, Sawhney V, 2006. Antioxidant defense mechanism under salt stress in wheat seedlings［J］. Biologia Plantarum, 50: 227—231
Miernyk JA, 1997. The 70kDa stressrelated proteins as molecular chaperones［J］. Trends in Plant Science, 2: 180—187
Mittler R, Zilinskas BA, 1991. Purification and characterization of pea cytosolic ascorbate peroxidase［J］. Plant Physiology, 97: 962—968
Mittler R, Zilinskas BA, 1994. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought［J］. The Plant Journal, 5: 397—405
Pratt WB, Krishna P, Olsen LJ, 2001. Hsp90binding immunophilins in plants: the protein movers［J］. Trends in Plant Science, 6: 54—58
Richter K, Buchner J, 2001. Hsp90: chaperoning signal transduction［J］. Journal of Cellular Physiology, 188: 281—290
Scandalios JG, 2005. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses［J］. Brazilian Journal of Medical and Biological Research, 38: 995—1014
Vani B, Saradhi PP, Mohanty P, 2001. Characterization of high temperature induced stress impairments in thylakoids of rice seedlings［J］. Indian Journal of Biochemistry and Biophysics, 38: 220—229
Wang WX, Vinocur B, Shoseyov O et al., 2004. Role of plant heatshock proteins and molecular chaperones in the abiotic stress response［J］. Trends in Plant Science, 9: 244—252
Weis E, Berry JA, 1988. Plants and high temperature stress［J］. Symposia of the Society for Experimental Biology, 42: 329—346
Xi X (席雪), Li SY (李树云), Yan N (严宁) et al., 2010. Photosynthetic response of Incarvillea zhongdiannensis (Bignoniaceae) subject to growth irradiance［J］. Acta Botanica Yunnanica (云南植物研究), 32: 519—527
Young JC, Moarefi I, Hartl FU, 2001. Hsp90: a specialized but essential proteinfolding tool［J］. Journal of Cell Biology, 154: 267—274
Zhang SB (张石宝), Hu H (胡虹), Wang H (王华) et al., 2005. The germplasm resources and exploitation of alpine ornamental plants in Yannan［J］. Chinese Wild Plant Resources (中国野生植物资源), 24: 19—22
Zhang Y, Mian MAR, Chekhovskiy K et al., 2005. Differential gene expression in Festuca under heat stress conditions［J］. Journal of Expetimental Botany, 56: 897—907
Zhang Y, Zwonitzer J, Chekhovskiy K et al., 2004. A functional genomics approach for identification of heat tolerance genes in tall fescue［J］. Molecular Breeding of Forage and Turf, 11: 87—96
Zhao J (赵剑), Yang WJ (杨文杰), Zhu WH (朱蔚华), 1999. Cytochrome P450 and plant secondary metabolism［J］. Chinese Bulletin of Life Sciences (生命科学), 11: 127—131

[1]	WANG Qi-Juan, CHEN Li-Gang, TU Di-Qiu. Overexpression of AtWRKY71 Affects Plant’s Defense Response to Pseudomonas syringae [J]. Plant Diversity, 2015, 37(05): 577-585.
[2]	YE Lin-Jiang- , ZHANG Zhi-Rong-, SUN Zhi-Xia-, TIAN Shuang. The Determination of Nuclear DNA Content (2Cvalue) on Some Representative Genus and Species of Magnoliaceae [J]. Plant Diversity, 2015, 37(05): 605-610.
[3]	HU Hui-Li, LI Bo, HU Wan, SUN Zhi-Xia, ZHANG Zhi-Yong, FAN Deng-Mei. Development and Characterization of Microsatellite Loci for Heavenly Bamboo (Nandina domestica) [J]. Plant Diversity, 2015, 37(2): 141-144.
[4]	XU Dao-Lan, CAO Jian-Guo, WANG Quan-Xi, DAI Xi-Ling. Cloning and Characterization of DEADbox RNA Helicases Gene from the Fern Equisetum arvense [J]. Plant Diversity, 2014, 36(06): 715-722.
[5]	QI Jing, DONG Zhen, ZHANG Yu-Xing. Cloning of ACC Oxidase Gene from Yali Pear and Tranformation of Its Antisense Expression Vector with Agrobacteriummediated Method [J]. Plant Diversity, 2014, 36(05): 622-628.
[6]	Lu Ping, GAO Li-Xia, JIN Feng, EN He-Ba-Ya-Er. Molecular Phylogeny of Ten Species of Oxytropis Based on psbAtrnH Sequences [J]. Plant Diversity, 2014, 36(03): 279-284.
[7]	LIU Bo, SHA Wei, ZHANG Mei-Juan, SONG Lu, AN Hong-Xue. Cloning and Expression Analysis of GpUCH Gene in Grimmia pilifera [J]. Plant Diversity, 2014, 36(03): 358-364.
[8]	XU Fang, ZHAO Shu, WU Rong-Ling, DU Fang. High Throughput SSR Multiplex Kits (12plex) for Euphrates’ Poplar, Populus euphratica (Salicaceae) [J]. Plant Diversity, 2014, 36(03): 365-374.
[9]	CHEN Lin-Yang, SONG Min-Shu, ZHA Hong-Guang, LI Zhi-Min. A Modified Protocol for Plant Genome DNA Extraction [J]. Plant Diversity, 2014, 36(03): 375-380.
[10]	XI Hou-Cheng, SUN Yao, XUE Chun-Ying. Molecular Phylogeny of Swertiinae (GentianaceaeGentianeae) Based on Sequence Data of ITS and matK [J]. Plant Diversity, 2014, 36(02): 145-156.
[11]	ZHANG Yi-Chi, LI Xia, GUO Zhen-Hua. The Effects of Preservation Methods and Storage Time on Estimating the Nuclear DNA Content (2C-value) of Bamboos [J]. Plant Diversity, 2014, 36(02): 227-232.
[12]	PAN Ye-Zhi, ZHANG Yi-Chi, GONG Xun, LI Fu-Sheng. Estimation of Genome Size of Four Panax Species by Flow Cytometry* [J]. Plant Diversity, 2014, 36(02): 233-236.
[13]	LIU Min-Lu-, YU Wen-Bin-, Wang-Hong. Rapid Identification of Plant Species and iFlora: Application of DNA Barcoding in a Large Temperate Genus Pedicularis (Orobanchaceae) [J]. Plant Diversity, 2013, 35(6): 707-714.
[14]	JIAO Li-Juan-, SHUI Yu-Min. Evaluating Candidate DNA Barcodes among Chinese Begonia (Begoniaceae) Species [J]. Plant Diversity, 2013, 35(6): 715-724.
[15]	LI Yan-Chun, WU Gang, YANG Zhu-Liang. DNA Barcoding of Edible Boletes (Boletaceae) from Yunnan, China [J]. Plant Diversity, 2013, 35(6): 725-732.