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25 April 1986, Volume 8 Issue 02
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  • Articles
    KARYOTYPE ANALYSIS OF TETRADOXA OMEIENSIS (ADOXACEAE)
    Liang Hanxing
    1986, 8(02):  1-3. 
    Abstract ( 2894 )   HTML ( )   PDF (149KB) ( 1319 )   Save
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    Karyotype anaiysis for the species Tetradoxa omeiensis (Hara) C.Y.Wu of the monotypic genus Tetradoxa C.Y.Wu is reported for the first.The number of the chromosones in the root-tip cell was found ot be 36.Of the 2n=36 fourteen chromosomes .
    THE OBJECTIVE REALITY OF SPECIES
    Hsu Pingsheng,Jin Desun
    1986, 8(02):  1-3. 
    Abstract ( 2527 )   HTML ( )   PDF (1821KB) ( 1541 )   Save
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    There has been in general two schools of thought with regard to the nature of species among naturalists and evolutionists. Some obervers hold that species are objectively real units in nature;others contend that they are artificial seem to be: Is there any consistent cirterion for the deignation of species?
    ONE NEW SPECIES OF LLOYDIA SALISB.FROM SICHUAN
    Liang Songyun
    1986, 8(02):  1-3. 
    Abstract ( 1740 )   HTML ( )   PDF (85KB) ( 1055 )   Save
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    Species affinis L. flavonutanti Hara,sed tepalis e reseo flavis basi non aurantiacis;filamentis glabris;ovario ovato apice obtuso; stigmate capitato differt.
    ONE NEW SPECIES OF LILIUM FROM SICHUAN
    Peng Longjin,Wang Binong
    1986, 8(02):  1-3. 
    Abstract ( 1977 )   HTML ( )   PDF (136KB) ( 1103 )   Save
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    Species L. taliensi et L.rosthornii affinis,a illa floribus faucibus purpuratis, glandibus viridibus utroque latere flavis, stylis germinibus duplo longioribus, a hac glandibus nectariferis margine efimbriatis,capsulis obovatis differt.
    A NEW SPECIES OF PINELLIA FROM ANHUI PROVINCE
    Liu Xiao-long,Guo Xinhu
    1986, 8(02):  1-3. 
    Abstract ( 2379 )   HTML ( )   PDF (133KB) ( 1219 )   Save
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    Species nova P.ternatae(Thunb.)Briet et P. pedatisectae Schott affinis,sed a P.ternata tubere tuberculato,petiolo sine tuberculis,foliorum segmentis latelalibus,saepe bilobis;a P. pedatisecta foliorum lamina trisecta non 6-11-pedatisecta differt.
    THE STUDY ON GENUS VANDA (ORCHIDACEAE) IN CHINA
    Cheng Shijum and Tang Zhenzi(C.Z.Tang)
    1986, 8(02):  1-3. 
    Abstract ( 2615 )   HTML ( )   PDF (480KB) ( 1322 )   Save
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    Vanda Jones,in Asiatic Researches 4:302,1795;R.Schultes,Generic names of Orchids 317,1963;H.Bechtel et al.,Manual Cult. Orch. Spp. 410,1980; O.Ames,Gen. and Spp. Philip. Orch., In Fam. Orch., Fasc. ..etc.
    AN ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR ABSCISIC ACID
    Ji Benren and Duan Jinyu
    1986, 8(02):  1-3. 
    Abstract ( 2646 )   HTML ( )   PDF (498KB) ( 1498 )   Save
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    We have devloped an enzyme-linked immunosorbent assay for quantitative analysis of abscisic acid (ABA) by coupling horseradish peroxi-dase (HRP)to ABA directly as an enzyme conjugate.The sensitivity of the assay,50 f mol, is comparable to the radioimmunoassay and the established methods using alkaline phosphatase (AP) -ABA or ABA-bovineserum albumin-AP as the enzyme conjugate.The logit B/B 0 value was linear between 12.5 pg to 100 ng. For improving assay sensitivity and precicion,we have also spproachedtion scheduies, injection doses and route, enzyme conjugate concentration and the dilution of antibody against ABA.
    RAPID MICR0-PR0PAGATI0N 0F EXPLANTS FR0M ADULT SEEDLING APPLE TREES IN VITR0
    Huanq Shizhou and Liu Aiqin
    1986, 8(02):  1-3. 
    Abstract ( 2497 )   HTML ( )   PDF (363KB) ( 1197 )   Save
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    The experimental reslllt showed: In the mcdium of Murashigr and Shoog (MS) or Schenk and Hidebrandt (SH) added with 6-benzylamino-purine (6 -BA) at 1- 2 mg/l, the sprouting rate of buds in subculture of the buds from the adult seedling app1e trees were over 92%. Buds multiplied 5-6.8 time in fifty days. The length of shoots in a rotating liquid culture was about three times more than that in the culture on agar-medium. In the medium added with indole- 3 -butyric acid (IBA) at 0.2-0.4 mg/l, the ro-oting rate of shoots were over 80%. The suryiyal rate of transplanting tuble- plantlets were more than 85%.
    STUDIES ON MABINLIN,THE SWEET PROTEIN FROM SEEDS OF CAPPARIS MASAIKAI N.STABILITY AND DENATURATION
    Ding Ming and Hu Zhong
    1986, 8(02):  1-3. 
    Abstract ( 2540 )   HTML ( )   PDF (939KB) ( 1391 )   Save
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    After a series of heating denatlltation experiments, we found the sweetness of Ma l was irreversibly lost in incubating at 80 0C for 30 minutes, the α-helix of Ma l also disappeared totally and the PAGE beha-vior changed.But the sweetness of Ma Ⅱwasm′t lost at 80oC for 48 h incubation, and its PAGE behavior didn′t change.
    ANALYSIS OFTHE CHEMICAL CONSTITUENTS OF OCIMUM GRATISSIMUM VAR.SUAVE OIL
    Yu Xuejian and cheng Biqiang
    1986, 8(02):  1-3. 
    Abstract ( 2644 )   HTML ( )   PDF (262KB) ( 1269 )   Save
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    This paper shows the chemical constituents of essential oil from Ocimum gratissimum Linn.var. suaue Willd., growing in Xishuangbanna,yunnan provice, by applying capillary GC/MD/DS on Finnigan-4510 type.
    THE QUANTITATIVE ANALYSIS OF DITERPENOIDS FROM RABDOSIA ERIOCALYX BY HPLC
    Ruan Dechun,Wang Dezu and Li Chundao
    1986, 8(02):  1-3. 
    Abstract ( 2510 )   HTML ( )   PDF (227KB) ( 1287 )   Save
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    This paper reported the qualitative and quantitative analysis of eriocalyxin A,eriocalyxin B,odonicin,maoecrystal B and maoecrystal Cfrom Rabdosia eriocalyx by HPLC method. The mithod was an initial test for quick microchromatography of diterpenoids from Rabdosia eriocaltyx.HPLC con-ditions as follow :Shinadzu Lc-4A, column, zorbax ODS 250 mmx4.6 mm;mobile phase,methanol-water (70:30);flow rate,0.65 ml/min; pressure,130kg/cm2;detectorSPD-2AS, WACE 230 NM.
    STUDIES ON THE CHEMICL CENEMICAL CONSTITUENTS OF CYNANCHUM SACCATUM
    Mu Quanzhang and Zhou Qianlan
    1986, 8(02):  1-3. 
    Abstract ( 2469 )   HTML ( )   PDF (269KB) ( 1260 )   Save
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    The chemical constituents,pharmacological action,clinical curative effict on epilepsy and domestication of Cynanchum otophyllum Schneid (Asciepiadaceae)have been reported.For expanding thedrug sources,we examined thechemical aonstiuents of the plant C.saccatum,collected atDeqin Xianof Yunnan provinca.
    STRUCTURE OF LUDONGNIN B
    Zheng Xinrong,Gao Academy o fHenan Prouine,Zhingzhou Sun Handong*and Lin Zhongwen
    1986, 8(02):  1-3. 
    Abstract ( 2663 )   HTML ( )   PDF (161KB) ( 1146 )   Save
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    Rabdosia rubescens Hara var.lushiensis Gao et Li (labiatae )which is widely distributed in theFuniushan west area of Henan,had BEEN used for the treatment of esphagel and cardial carcinoma in Henan.We have reported the isolation and the structural determination of ludongnin A from the ethereal extract of the dry leaves of this plant.The present paper, we report studies on a new crystalline diterpenoid, ditrpenoid, ludongnin B[C 20 H 26 O 5 ,MP 296-9.C]which is a minor component isolated from the same plant and its structure has been shown to be ent-6,7-spirosece-7,20-δ-lactonic ring- 6, 19-γ-lactonic ring-16-kaurane-15-one (1)on the basis of spectrodcopic evidence and compared with fnown compound, ludongini A.
    DITERPENOIDS OF RABDOSIA ERIOCALYX VAR.LAXIFLORA
    Shen Peiqiong Yunnan lnstitute of tropical Botany,Academia Sinica)Sun Handong*and Lin Zhongwen
    1986, 8(02):  1-3. 
    Abstract ( 2730 )   HTML ( )   PDF (313KB) ( 1357 )   Save
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    During the course ofour studies on the biologically active con-stituents of Rabdosia plants,seven diterpenoids were isolated from the ether extract of the leaves ofRabdosia eriocalyx (Dunn) Hara var .laxifiora C.Y.Wu et H.LI COLLECTED FROM Xishuangbanna in Yunnan province.Four of them haem have been established as eriocalyxin B (=ent -6α,7α-dihydroxy-7β,20-epoxykaur-16-en -2, 3-ethenylene-l, 15-dione)(1) ,maoecrystai A(-ent-6α-hydroxy-15α-acetoxy-3β 20-epocalyxin-16-en-l,7-dione)(2,maoecrystal B(=ent-6α,7α-dihydroxy-15α-acetoxy-7β,20-epoxy-kaur-16-en-2,3-ethenylene-l-one)(3), and oridonin (4)from spectroscopic data,respectively.
    INVTIGATION OFTHE SEXUAL PROCESS IN INTERSECIFIC CROSSES BETWEEN CAMELLIA PITARDII PITARDII VAR. YUNNANENSIS AND C.CHRYSANTHA
    Liang Hanxing,Zhang Zanying and Zhang Xianglan
    1986, 8(02):  1-3. 
    Abstract ( 3032 )   HTML ( )   PDF (468KB) ( 1292 )   Save
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    An investigation of the sexual process in interspecific crosses bitween Camellia pitardll var .yunnanensis and C.chrysantha is described in the present work.The results are summsrized as follows: 1.Germination of pollen grains on the stigma in crosses occurs 4 hours after artificial pollination.Thet of control.
    FLAVONOID CONSTITUENTS FROM OIL CAKE OF THE SEEDS OF CAMELLIS OLEIFERA
    Wang Zhe ,Jia Zhongjian,Chu Tzetsin (Chemistry Department of Lanzhow Uniuersity )Yang Chongren* and Zhou Jun
    1986, 8(02):  1-3. 
    Abstract ( 2650 )   HTML ( )   PDF (224KB) ( 1023 )   Save
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    Two flavonoid glycosides,kaempferol-3-O-β-D-glucopyrano-syl-(6→1)-O-α-L-rhamnopynanoside (1)and kaempferol-3-O-β-D-glucopyranosyl-[(2→1)-O-α-L-rham-nopyranoside (2)were isolated from the buthanol extracts of oil cake of the seeds of Camellia oleifera Abel., and identified by means of 1H NMR,UV,EL-MS,FAB-MS as well as 13C NMR,together sucrose.
    ULTRASTRUCTURAL STUDY OF MLCROSPOROGENESIS IN PINUS YUNNANENSIS
    Liu Chengyun,Li Song Xu Weiming and Li Ming
    1986, 8(02):  1-3. 
    Abstract ( 2981 )   HTML ( )   PDF (714KB) ( 1146 )   Save
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    The contractiaction of the protoplasts during the early development of the microsporangium os observed and the callose wall is fprmed at the same time.The microspor mother cells are separated from each other and were surrounded by callose wall before meiosis.The small pore about 0.2 to 0.3micron in diameter wihin the callose wall was observed.After the tetrad was formed,the nuclear evelope and the golgi bodies show a particular activity which is provably related to the exine depositing.
    A BRIEF REPORT OF DEVELOPMENT OF GAMETOPHYTES IN WOODSIA
    Ma Yilun and Wang Fuxiong (Wang Fu-hsiung)
    1986, 8(02):  1-3. 
    Abstract ( 2497 )   HTML ( )   PDF (434KB) ( 1394 )   Save
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    ln the present paper the onlogeneses of gametophytes on Wo-odsia subcordata Turcz.,W. iluensis R. Br. and W.polystichoides Eat.have been reported briefiy. The gametophytes ussed for experimental studies were obtained by means of culture from spores in the medium containingmineral components.
    EMBRYOLOGICAL STUDIES OF STYRAX SUBERIFOLIUS
    Ye Xiulin, Qian Nanfen and Wang Fuxiong (Wang Fu-hsiung)
    1986, 8(02):  1-3. 
    Abstract ( 2495 )   HTML ( )   PDF (532KB) ( 1183 )   Save
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    The present paper deals with the development of male and fe-wale gametophytes and the early enbryogenesis of Styrax suberifolius.The ovary 20-23 ovules.The sovules are hemitropous,with 2 imtigu-ments,tenuinucellate and the archesporial cell functions directly as the mega-spore mother cell.The chalazal megaspore is functional.The development ofthe embtyo sac conforms to the normal type.The mature embryo sac is rich in starch grains.The formation of the microspores are of the simulyameous type and the mature pollen grain is 2-celled.