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Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 779-783.doi: 10.7677/ynzwyj201313197

• 研究论文 • 上一篇    下一篇

构建基于磁珠微流控芯片的iFlora遗传信息高效获取系统

 马莉萍1, 伊廷双2, 左显维1, 张彪1, 李云霞1, 韩根亮1   

  1. 1 甘肃省科学院传感技术研究所,甘肃 兰州730000; 2 中国科学院昆明植物研究所
    中国西南野生生物种质资源库,云南 昆明650201
  • 收稿日期:2013-09-30 出版日期:2013-11-25 发布日期:2013-10-16
  • 基金资助:

    中国科学院科技创新“交叉与合作团队”  (31129001);科技部国家高技术研究发展计划 (863计划) 主题项目 (2012AA021801);中国科学院大科学装置项目 (2009-LSFGBOWS-01)

A Noval Magnetic Beadbased Microchip for iFlora Genetic Information Efficient Acquisition System*

 MA  Li-Ping-1, YI  Ting-Shuang-2, ZUO  Xian-Wei-1, ZHANG  Biao-1, LI  Yun-Xia-1, HAN  Gen-Liang-1   

  1. 1 Institute of Sensor Technology, Gansu Academy of Sciences, Lanzhou 730000, China; 2 Germplasm Bank of Wild Species,
    Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2013-09-30 Online:2013-11-25 Published:2013-10-16
  • Supported by:

    中国科学院科技创新“交叉与合作团队”  (31129001);科技部国家高技术研究发展计划 (863计划) 主题项目 (2012AA021801);中国科学院大科学装置项目 (2009-LSFGBOWS-01)

摘要:

磁珠以其比表面积大、易与生物分子耦联、操控方便等优点,在生命科学中得到了广泛应用。随着微机电系统(Micro Electro Mechanical Systems, MEMS)技术的发展,将磁珠应用到微流控芯片中构建磁珠微流控分析系统,为生物样品分离、检测提供了一种全新方法。新一代植物志iFlora融入现代DNA测序技术,应用高速发展的信息、网络技术及云计算分析平台,收集、整合和管理植物物种相关信息,以实现物种智能鉴定和数据提取,而包括DNA条形码在内的遗传信息及其获取技术在 iFlora中的作用至关重要。本文重点概述了基于纳米磁珠的微流控芯片技术及其在分子生物学领域中的应用,提出构建基于纳米磁珠微流控芯片的iFlora遗传信息采集系统,在微芯片上完成从DNA提取到测序全过程,实现物种遗传信息的快速、高效获取。

关键词: 磁珠, 微流控芯片, DNA条形码, 新一代植物志, 遗传信息

Abstract:

 Magnetic Bead (MB) has been widely applied in life science for its high surface area, coupling capacity with biomolecules and convenience of being manipulated. With the development of Micro Electro Mechanical systems (MEMS) technologies, MBbased biomicrosystem provide a new approach for separation and detection of biological samples. Integrating modern DNA sequencing technologies and applying highspeed computer digitization, network technology and cloud computing analysis platform for collection and management of species information, the nextgeneration Flora, or iFlora is an intelligent application system for plant species identification and related information acquisition. Efficient genetic information acquiring technology is key in building iFlora. This paper briefly introduces the MBbased biomicrosystem technology and its application in molecular biology. The authors suggest a new MBbased biomicrosystem iFlora genetic information collection system. Combined with MB technology, the total analyses from cell lysis to DNA sequencing will be achieved on microchip, which will realize the quick and efficient acquirement of genetic information.

Key words: Gnetic bead, Microchip, DNA barcodes, iFlora, Genetic information

中图分类号: 

  • TP 212.2