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Plant Diversity ›› 2013, Vol. 35 ›› Issue (6): 769-773.DOI: 10.7677/ynzwyj201313198

• 研究论文 • 上一篇    下一篇


 唐敏1, 伊廷双2, 王欣1, 谭美华1, 周欣1   

  1. 1 深圳国家基因库,深圳华大基因研究院,广东 深圳518083; 2 中国科学院昆明植物研究所
    中国西南野生生物种质资源库,云南 昆明650201
  • 收稿日期:2013-09-30 出版日期:2013-11-25 发布日期:2013-10-12
  • 基金资助:

    中国科学院科技创新“交叉与合作团队”  (31129001);科技基础性工作专项重点项目 (2013FY112600);国家高技术研究发展计划 (863计划) (2012AA021601)

The Application of Metabarcoding Technology in Identification of Plant Species Diversity

 TANG  Min-1, YI  Ting-Shuang-2, WANG  Xin-1, TAN  Mei-Hua-1, ZHOU  Xin-1   

  1. 1 China National GeneBankShenzhen, BGIShenzhen, Shenzhen 518083, China; 2 Germplasm Bank of Wild Species
    in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2013-09-30 Online:2013-11-25 Published:2013-10-12


目前植物学研究已进入后植物志时代,iFlora的实现需要以传统植物分类学及相关研究为基础,整合基于高通量测序的DNA条形码(DNA barcoding)技术并开发便携式快速鉴定仪器及构建信息平台。传统植物鉴定多基于形态学分类,而近年来快速发展的DNA条形码快速鉴定技术被各界分类学家认可,在植物鉴定中也被广泛应用。但DNA条形码技术仍存在一些问题亟待解决,如种的鉴定需要多个条形码的解析、Sanger测序平台无法处理混合样品。本文介绍了传统植物分类技术和DNA条形码技术在植物研究中的应用和遇到的瓶颈;并重点介绍了基于高通量测序的metabarcoding技术在植物鉴定及多样性研究中的应用及前景,及其与iFlora的关系。

关键词: 植物志, 植物多样性, DNA条形码, iFlora, metabarcoding 技术


Botany has entered a postFlora era. Benefited from traditional plant taxonomy and related studies, the success of iFlora requires the integration of DNA barcoding through state of art highthroughputsequencing technologies and portable equipment for rapid taxonomic identification and information platform. Traditional plant identification relies mostly on morphological features, while DNA barcoding has been widely applied as a complementary molecular approach to facilitating fast species identification. However, issues remain in classic DNA barcoding, e.g., specieslevel resolution can only be achieved via multiple barcode markers; taxon identity cannot be revealed for pooled plant samples via Sanger sequencing. In this review, the authors introduce recent developments in metabarcoding, which is based on the nextgenerationsequencing technologies, and its applications in a few key aspects in plant diversity and its potential role in iFlora.

Key words: Flora, Plant diversity, DNA barcoding, iFlora, Metabarcoding techonology