Phylogeny of the Chinese Endemic Fern Genus Neocheiropteris (Polypodiaceae)

doi:10.3724/SP.J.1143.2011.10188

Abstract

Abstract:

Neocheiropteris is one of the four Chinese endemic fern genera. The taxonomic circumscription and systematic position of Neocheiropteris have always been argued among taxonomists since its establishment by Christ in 1905. The anatomical examination of Neocheiropteris and Neolepisorus is performed, and four chloroplast DNA markers: rbcL, rpS4 & rpS4-trnS IGS, trnL intron & trnL-trnF IGS and atpB & atpB-rbcL IGS for Neocheiropteris and its closely related taxa, totally 14 taxa are sequenced. Phylogenetic trees are constructed with the Maximum-parsimony, Maximum-likelihood and Bayesian inference method based on coded dataset/non-coded dataset/combined dataset. The division of Neocheiropteris and Neolepisorus is supported by both anatomical and molecular phylogeny evidence. Anatomical research provides support for the division of the two genera by their totally different characters of the division back and vein of frond, and for the close connection of the two genera by their common characters of rhizome scale. Molecular phylogeny research shows that: Neocheiropteris is sister to a clade that includes Tricholepidium and Lepidomicrosorium, and Neolepisorus is sister to the combination of above-mentioned three genera. The taxonomic circumscription and systematic position of Neocheiropteris are investigated by integrating anatomical characters and molecular phylogenetic results, only Neocheiropteris palmatopedata and N.triglossa should be treated as members of Neo-cheiropteris. The systematic position of N.triglossa is investigated based on molecular together with anatomical evidence for the first time.

Key words: Neocheiropteris, Phylogeny, Anatomy, Chloroplast DNA sequences

CLC Number:

Q 949.36

DU Xin-Yu- , CHENG Xiao. Phylogeny of the Chinese Endemic Fern Genus Neocheiropteris (Polypodiaceae)[J]. Plant Diversity, 2011, 33(3): 261-268.

References

林尤兴, 张宪春, 陆树刚等, 2000. 中国植物志(第6卷第2分册)［M］. 北京: 科学出版社
Bosman MTM, 1991. A monograph of the fern genus Microsorum (Polypodiaceae)［J］. Leiden Botanical Series, 14: 1—161
Ching RC, 1933. The studies of Chinese ferns Ⅸ［J］. Bulletin of the Fan Memorial Institute of Biology, 4 (3): 103—107
Ching RC, 1940. The studies of Chinese ferns ⅩⅩⅩ［J］. Bulletin of the Fan Memorial Institute of Biology, 10 (1): 1—22
Christ H, 1905. Fougeres de Mengtze［J］. Bulletin de l′herbier Boissier, 62 (1): 21—22
Doyle JJ，Doyle JL, 1987. A rapid DNA isolation procedure for smal1 quantities of fresh leaf tissue［J］. Phytochemical Bulletin, 19: 11—15
Hasebe M, Worf PG, Pryer KM et al., 1995. Fern phylogeny based on rbcL nucleotide sequences［J］. American Fern Journal, 85 (4): 134—181
Korall P, Pryer KM, Metzgar JS et al., 2006. Tree ferns: Monophyletic groups and their relationships as revealed by four protein-coding plastid loci［J］. Molecular Phylogenetics and Evolution, 39: 830—845
Kreier HP, Zhang XC, Muth H et al., 2008. The microsoroid ferns: Inferring the relationships of a highly diverse lineage of Paleotropical epiphytic ferns (Polypodiaceae, Polypodiopsida)［J］. Molecular Phylogenetics and Evolution, 48: 1155—1167
Liu HM (刘红梅), Zhang XC (张宪春), Zeng H (曾辉), 2009. Application of DNA Sequences in Pteridophyte Phylogenetic Study［J］. Journal of Integrative Plant Biology, 44 (2): 143—158
Nadot S, Bittar G, Carter L et al., 1995. A phylogenetic analysis of monocotyledons based on the chloroplast gene rps4, using parsimony and a new numerical phenetics method［J］. Molecular Phylogenetics and Evolution, 4: 257—282
Posada D, Crandall KA, 1998. Modeltest : testing the model of DNA substitution［J］. Bioinformatics, 14 (9) : 817─818
Pryer KM, Schuettpelz E, Wolf PG et al., 2004. Phylogeny and evolution of ferns(monilophytes)with a focus on the early leptosporangiate divergences［J］. American Journal of Botany, 91 (10): 1582—1598
Ronquist F, Huelsenbeck JP, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models［J］. Bioinformatics, 19 (12): 1572─1574
Schneider H, Schuettpelz E, Pryer KM et al., 2004. Ferns diversified in the shadow of angiosperms［J］. Nature, 428: 553—557
Schuettpelz E, Pryer KM, 2007. Fern phylogeny inferred from 400 leptosporangiate species and three plastid genes［J］. Taxon, 56 (4): 1037—1050
Shi L (石雷), Zhang XC (张宪春), 1999. Taxonomic studies of the fern genus Lepidomicrosorum Ching et Shing (Polypodiaceae) from China and neighboring regions［J］. Acta Phytotaxonomica Sinica (植物分类学报), 37 (5): 509—522
Smith AR, Cranill RB, 2002. Intrafamilial relationships of the Thelypteroid ferns (Thelypteridaceae)［J］. American Fern Journal, 92: 131—149
Swofford DL, 2002. PAUP*. Phylogenetic analysis using parsimony (* and other methods), version 4.0b10 (Alvitec)［CP］. Sunderland, Massachusetts: Sinauer AssociatesTagawa M, 1952. Fern miscellany (6)［J］. The Journal of Japanese Botany, 27 (7): 217─218
Tarberlet P, Gielly L, Pautou G et al., 1991. Universal primers for amplification of the three noncoding regions of chloroplast DNA［J］. Plant Molacular Biology, 17: 1105—1109
Thompson JD, Gibson TJ, Plewniak F et al., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools［J］. Nucleic Acids Research, 25 (24): 4876─4882
Trewick SA,Morgan-Richards M,Russell SJ et al., 2002. Polyploidy phylogeography and Pleistocene refugia of the rockfern Asplenium ceterach: evidence from chloroplast DNA［J］. Molecular Ecology, 11: 2003—2012
Wang L, Qi XP, Xiang QP et al., 2010b. Phylogeny of the paleotropical fern genus Lepisorus (Polypodiaceae, Polypodiopsida) inferred from four chloroplast genome regions［J］. Molecular Phylogenetics and Evolution, 54: 211—225
Wang L, Wu ZH, Xiang QP et al., 2010a. A molecular phylogeny and a revised classification of tribe Lepisoreae (Polypodiaceae) based on an analysis of four plastid DNA regions［J］. Botanical Journal of the Linnean Society, 162: 28—38
Wolf PG, 1997. Evaluation of atpB nucleotide sequences for phylogenetic studies of ferns and other Pteridophytes［J］. American Journal of Botany, 84 (10): 1429—1440

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