Plant Diversity ›› 2011, Vol. 33 ›› Issue (5): 535-539.DOI: 10.3724/SP.J.1143.2011.11004

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The Development of Nuclear Microsatellite Markers for an Endangered Plant, Sinomanglietia glauca (Magnoliaceae)

 XIONG  Min-1, WANG  Jing-1, ZHANG  Zhi-Rong-3, ZHANG  Zhi-Yong-1、2   

  1. 1 Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China; 2 Key State Laboratory
    of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
    3 China Germplasm Bank of Wild Species/Key Laboratory of Biodiversity and Biogeography,
    Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2011-01-07 Online:2011-10-25 Published:2011-03-03
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Sinomanglietia glauca is an endangered species found in Yichun of Jiangxi Province and Yongshun of Hunan Province. To further explore its population genetic characteristics, we developed seven microsatellite markers from S.glauca using a FIASCO (fast isolation by AFLP sequences containing repeats) method. The number of alleles (NA) ranged from two to seven in 16 samples of four wild populations. The ranges of observed (HO) and expected (HE) heterozygosities were 0.079-0.989 and 0.187-0.600, respectively. The transferability of the seven microsatellite loci was tested upon nine related species of S.glauca within Magnoliaceae. Among which, two (28.5%, Michelia macclurei) to six (85.7%, Tsoongiodendron odorum) loci were successfully amplified. These polymorphic microsatellite markers could be used to decipher the genetic structure, breeding system and population history of S.glauca as well as other relatives within Magnoliaceae.

Key words: Sinomanglietia glauca, Microsatellite, Molecular marker, Transferability

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