The Development of Nuclear Microsatellite Markers for an Endangered Plant, Sinomanglietia glauca (Magnoliaceae)

doi:10.3724/SP.J.1143.2011.11004

Abstract

Abstract:

Sinomanglietia glauca is an endangered species found in Yichun of Jiangxi Province and Yongshun of Hunan Province. To further explore its population genetic characteristics, we developed seven microsatellite markers from S.glauca using a FIASCO (fast isolation by AFLP sequences containing repeats) method. The number of alleles (NA) ranged from two to seven in 16 samples of four wild populations. The ranges of observed (HO) and expected (HE) heterozygosities were 0.079-0.989 and 0.187-0.600, respectively. The transferability of the seven microsatellite loci was tested upon nine related species of S.glauca within Magnoliaceae. Among which, two (28.5%, Michelia macclurei) to six (85.7%, Tsoongiodendron odorum) loci were successfully amplified. These polymorphic microsatellite markers could be used to decipher the genetic structure, breeding system and population history of S.glauca as well as other relatives within Magnoliaceae.

Key words: Sinomanglietia glauca, Microsatellite, Molecular marker, Transferability

CLC Number:

Q 78

XIONG Min-, WANG Jing-, ZHANG Zhi-Rong-, ZHANG Zhi-Yong. The Development of Nuclear Microsatellite Markers for an Endangered Plant, Sinomanglietia glauca (Magnoliaceae)[J]. Plant Diversity, 2011, 33(5): 535-539.

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