Plant Diversity ›› 2011, Vol. 33 ›› Issue (4): 383-388.DOI: 10.3724/SP.J.1143.2011.11029

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MS1516 is Essential for Tetrad Formation and Microspore Development in Arabidopsis thaliana

 ZHAO  Xian-Ling, CHANG  Hai-Shuang, YANG  Zhong-Nan, ZHANG  Sen   

  1. College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China
  • Received:2011-02-18 Online:2011-08-25 Published:2011-05-03


Anthers in flowers produce via meiosis haploid microspores, which mature to become pollen. However, pollen development at the molecular level is still not very clear. For the purpose of gaining understanding of the molecular control of pollen development, an Arabidopsis partial malesterility mutant ms1516 was reported previously, and the mutant gene MS1516 was mapped to a region of 28kb on chromosome 3 by a mapbased cloning approach. Here, we report that further bioinformatics analysis revealed that there was a meiosis related gene AtATM in this region. Allelism tests indicated that MS1516 and AtATM belonged to the same locus. Cell biology analysis showed that ms1516 mutant produced multiple uneven microspores during anther development, and most of which aborted at late stages. DAPI staining indicated that chromosome segregation was abnormal in ms1516 mutant meiocytes. Further scanning electron microscopy examination revealed abnormal pollen morphology formation in ms1516 mutant. Collectively these results demonstrated that MS1516 played an important role in tetrad formation and microspore development.

Key words: Arabidopsis thaliana, Male sterile, Tetrad, Meiosis, MS1516

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