Proteomics Reveal the Molecular Mechanism of Arabidopsis thaliana on Bacterial Signal N-3-oxo-hexanoyl-homoserine-lactone (OHHL)

doi:10.3724/SP.J.1143.2011.10229

Abstract

Abstract:

The populational signals derived from bacteria can induce plant resistance to pathogen or regulate plant development. Here we used 2D proteomics method to investigate Arabidopsis thaliana seedlings responding to OHHL, one of bacterial popular signals. After 2D proteomics and assay, we obtained 47 differential protein spots, most of their expression show increase with the extension of treatment time; these proteins are also involved in plant antioxidant activity, material metabolism and signal transaction. Therefore, we believe our results will show more insight into understanding the interactions mechanism of plant and microbe, and further well regulate plant themselves growth and development.

Key words: Proteomics, Arabidopsis thaliana, Bacterial signal, OHHL

CLC Number:

Q 942

NIU Xue-Yan-, ZHANG Hai-Jun-, BAI Xue-Gui-, LI Wan-Sha-, LIU Xiao-Guang-, HU Xiang-Yang. Proteomics Reveal the Molecular Mechanism of Arabidopsis thaliana on Bacterial Signal N-3-oxo-hexanoyl-homoserine-lactone (OHHL)[J]. Plant Diversity, 2011, 33(4): 389-395.

References

Alison A, 1999. A postgenomic challenge: learning to read patterns of protein synthesis［J］. Nature, 402: 915
An XH (安贤惠), 1998. The progress of Arabidopsis thalianaand its study［J］. Acta Agriculturae Borealioccidentalis Sinica (西北农业学报), 7 (1): 92—94
Bashandy T, Guilleminot J, Vernoux T et al., 2010. Interplay between the NADPlinked thioredoxin and glutathione systems in Arabidopsis auxin signaling［J］. The Plant Cell, 22:376
Bradford MM, 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of proteindye binding［J］. Analytical Biochemistry, 72: 248—254
Candiano G, Bruschi M, Musante L et al., 2004. Blue silver: a very sensitive colloidal coomassie G250 staining for proteome analysis［J］. Electrophoresis, 25: 1327—1333
CoskunAri FF, BosgelmezTinaz G, 2008. GrlA and gyrA mutations and antimicrobial susceptibility in clinical isolates of ciprofloxacin methicillinresistant Staphylococcus aureus［J］. European Journal of Medical Research, 3 (8): 366—370
Engebrecht J, Nealson K, Silverman M, 1983. Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri［J］. Cell, 32 (3): 773—781
Gao M, Chen H, Eberhard A et al., 2007. Effects of AiiAmediated quorum quenching in Sinorhizobium meliloti on quorumsensing signals, proteome patterns, and symbiotic interactions［J］. Molecular PlantMicrobe Interactions, 20 (7): 843—856
Gorg A, Obermaier C, Boguth G et al., 2000. The current state of twodimension electrophoresis with immobilized pH gradients［J］. Electrophoresis, 21 (6): 1037—1053Gu RS (谷瑞升), Liu QL (刘群求), Cben XM (陈雪梅) et al., 1999．An economical and effective method for twodimensional electrophoresis of proteins of woody plant［J］. Journal of Beijing Forestry University (北京林业大学学报), 21 (5): 1—7
Li XM (李雪梅), Li Y (李莹), Ma LJ (马莲菊) et al., 2009. Progress research in on rice proteomics under stress［J］. Journal of Shenyang Normal University (沈阳师范大学学报), 27 (3): 257—263
Li S, Xue L, Xu S et al., 2009. Hydrogen peroxide acts as a signal molecule in the adventitious rootformation of mung bean seedlings［J］. Environmental and Experimental Botany, 65: 63—71
Liu X, Bimerew M, Ma Y et al., 2007. Quorumsensing signaling is required for production of the antibiotic pyrrolnitrin in a rhizospheric biocontrol strain of Serratia plymuthica［J］. FEMS Microbiology Letter, 270 (2): 299—305
Liu X, Jia J, Atkinson S et al., 2010. Biocontrol potential of an endophytic Serratia sp. G3 and its mode of action［J］. World Journal of Microbiology and Biotechnology, 26: 1465—1471
Mathesius U, Mulders S, Gao M et al., 2003. Extensive and specific responses of a eukaryote to bacterial quorumsensing signals［J］. Proceedings of the National Academy of Sciences of the United States of America, 100 (3): 1444—1449
O’Farrell PH, 1975. High resolution twodimensional electmphoresis of proteins［J］. The Journal of Biological Chemistry, 250 (10): 4007—402l
OrtizCastro R, MartinezTrujillo M, LopezBucio J, 2008. NacylLhomoserine lactones: a class of bacterial quorumsensing signals alter postembryonic root development in Arabidopsis thaliana［J］. Plant Cell and Environment, 31: 1497—1509
Reading NC, Sperandio V, 2006. Quorum sensing: the many languages of bacteria［J］. FEMS Microbiology Letter, 254 (1): 1—11
Wang XQ, Yang PF, Liu Z et al., 2009. Exploring the mechanism of physcomitrella patens desiccation tolerance through a proteomic strategy［J］. Plant Physiology, 149:1739—1750

[1]	Fei-Hong Yan, Li-Ping Zhang, Fang Cheng, Dong-Mei Yu, Jin-Yong Hu. Accession-specific flowering time variation in response to nitrate fluctuation in Arabidopsis thaliana [J]. Plant Diversity, 2021, 43(01): 78-85.
[2]	Mulan Wang a, b, , Yunmei Shen c, , Faqing Tao b, Shengchao Yang a, Weiqi Li b. Submergence induced changes of molecular species in membrane lipids in Arabidopsis thaliana [J]. Plant Diversity, 2016, 38(03): 156-162.
[3]	Xiong Li,§, Yunqiang Yang,§, Shihai Yang,§, Xudong Sun, Xin Yin, . Comparative proteomics analyses of intraspecific differences in the response of Stipa purpurea to drought [J]. Plant Diversity, 2016, 38(02): 124-145.
[4]	LI Xiong, YANG Yun-Qiang, YANG Shi-Hai, MA Lan, KONG Xiang-Xiang, HU Xiang-Yang, YANG Yong-Ping. Protein Level Analysis of Kobresia pygmaea (Cyperaceae) Response to Diurnal Environment on the Tibetan Plateau [J]. Plant Diversity, 2015, 37(2): 145-156.
[5]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Changes of Membrane Stability in PotassiumStressed Plants [J]. Plant Diversity, 2014, 36(05): 595-602.
[6]	JIA Yan-Xia, TAO Fa-Qing, LI Wei-Qi. Analysis of Endogenous Hormone Levels to Reveal the Retardation by Suppression of Phospholipase Dα1 in Arabidopsis Leaves during Hormonepromoted Senescence [J]. Plant Diversity, 2014, 36(04): 485-496.
[7]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Plants Adapt to LongTerm Potassium Deficiency by Accumulation of Membrane Lipids in Leaves and Maintenance of Lipid Composition in Roots [J]. Plant Diversity, 2014, 36(02): 163-176.
[8]	CHENG Zhi-Peng, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. ST273 Is Essential for Tapetum and Microspore Development in Arabidopsis thaliana* [J]. Plant Diversity, 2012, 34(5): 502-508.
[9]	LIN Yuan-Yuan- , YU Di-Qiu. Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments [J]. Plant Diversity, 2012, 34(5): 509-518.
[10]	LIANG Peng-, KONG Xiang-Xiang-, WANG Chun-Tao-, ZHA Xiang-Dong-, HU Xiang-Yang. Modification of pFGC5941 Construct and Transfer of Double Antisense Genes of NAC1 and SIP1 into Arabidopsis thaliana [J]. Plant Diversity, 2012, 34(4): 403-.
[11]	ZHAO Juan, CHEN Yan, HUI Sheng-Ming, TU Wei-Yi, CHEN Chen, GAO Ju-Fang. Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation [J]. Plant Diversity, 2012, 34(2): 164-170.
[12]	LI Wan-Sha, WANG Chun-Tao, HU Xiang-Yang. Isolation and Identification of an Arabidopsis thaliana Mutant with Serration Leaf Margin [J]. Plant Diversity, 2012, 34(01): 28-32.
[13]	GUO Thao-, LI Wan-Sha-, ZHA Xiang-Dong. [J]. Plant Diversity, 2011, 33(6): 645-652.
[14]	ZHAO Xian-Ling, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. MS1516 is Essential for Tetrad Formation and Microspore Development in Arabidopsis thaliana [J]. Plant Diversity, 2011, 33(4): 383-388.
[15]	YANG Hui- , GAO Bo-, LI Wei-Qi. Lipid Peroxidation Induced by Metal Ion Stress is Positively Related to Lonic Polarization in Plants [J]. Plant Diversity, 2011, 33(3): 293-298.