Assessment of Genetic Variation and Differentiation of Ligularia tongolensis (Compositae) Detected by ISSRs

Plant Diversity ›› 2007, Vol. 29 ›› Issue (05): 537-542.

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Assessment of Genetic Variation and Differentiation of Ligularia tongolensis (Compositae) Detected by ISSRs

WANG Jin-Feng^{1 , 2} , Chiaki kuroda³ , GONG Xun¹

1 Kunming Institute of Botany , Chinese Academy of Sciences , Kunming 650204, China ;
2 Graduate School of the Chinese Academy of Sciences, Beijing 100049, China ;
3 Department of Chemistry, Rikkyo University , Tokyo 171 - 8501 , Japan

Received:2006-12-30 Online:2007-10-25 Published:2007-10-25
Contact: GONG Xun

Abstract

Abstract: The genetic diversity of Ligularia tongolensis ( Franch.) Hand.-Mazz. was analyzed by inter-simple sequence repeat markers (ISSR). Eight informative and reliable primers were chosen from100 pre-screened primers, of which, 148 ISSR loci were polymorphic. Genetic diversity was high with PPB = 50. 45%, H = 0.1595 and I = 0.2440 at population level; PPB = 88.10% , H = 0.2811 and I = 0.4279 at species level. The G_st = 0.4355 indicated a high degree of genetic differentiation occurred among L. tongolensis populations. The main factor responsible for the high level genetic diversity and genetic differentiation among populations ascribed to diversified habitats diversification. Gene flow was inhibited by its habitats discontinuity which finally caused the genetic differentiation .

Key words: Ligularia tongolensis

CLC Number:

Q 16

WANG Jin-Feng^, , Chiaki kuroda , GONG Xun. Assessment of Genetic Variation and Differentiation of Ligularia tongolensis (Compositae) Detected by ISSRs[J]. Plant Diversity, 2007, 29(05): 537-542.

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Assessment of Genetic Variation and Differentiation of Ligularia tongolensis (Compositae) Detected by ISSRs

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