Plant Diversity ›› 2015, Vol. 37 ›› Issue (05): 595-604.doi: 10.7677/ynzwyj201515004

• Articles • Previous Articles     Next Articles

Genetic Diversity Analysis of Nelumbo Accessions Based on ESTSSR Markers

 徐玉仙1、2、3, 张微微2、4, 莫海波2, 李春2、3, 曹建国1, 田代科2、3   

  1. 1 College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China; 2 Shanghai Chenshan
    Plant Science Research Center, Chinese Academy of Sciences, Chenshan Botanical Garden, Shanghai 201602, China;
    3 Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai 201602, China;
    4 Shanghai Vocational College of Agriculture and Forestry, Shanghai 201600, China
  • Received:2015-01-06 Online:2015-09-25 Published:2015-03-16
  • Supported by:

    上海市绿化和市容管理局攻关项目 (F112421)、上海市绿化和市容管理局科学技术项目 (G142427)

Abstract:

EST-SSR markers were applied to estimate the genetic diversity for 30 accessions of Nelumbo nucifera, 6 accessions of Nelumbo lutea and 14 hybrids between these two species. The 52 of 123 ESTSSR markers (423%) were screened and then applied to amplify the 50 Nelumbo accessions. A total of 177 alleles were identified, and the number of alleles per locus and Polymorphic Information Content (PIC) value varied from 2 to 8 with an average of 34 alleles and from 063 (NNFB1059) to 091 (NNFB750) with an average value of 079, respectively. Jaccard similarity coefficients of the amplification results were analyzed by NTSYSpc2.11 software and the genetic similarity coefficient was from 024 to 086. The clustering dendrogram constructed by UPGMA method indicated that 50 accessions of Nelumbo could be divided into four major groups at the similarity coefficient of 037. Group I and group II included Nnucifera; group III included the majority of AsianAmerican lotus hybrids; and group IV included Nlutea. In addition, the AsianAmerican hybrids were closer to Nnucifera based on genetic relationship, which is consistent with the traditional classification result and the previous reports.

Key words: Nelumbo, EST-SSR, Genetic diversity, Genetic relationship

CLC Number: 

  • Q 16