Plant Diversity ›› 2010, Vol. 32 ›› Issue (S17): 74-80.

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Study on the Sampling Strategy of Sinocalycanthus chinensis for Exsitu Conservation of Genetic Diversity

 CHEN  Xiang-Bo-1, TIAN  Qi-2   

  1. 1 Shanghai Landscape Gardening Research Institute, Shanghai 200232, China;
    2 Chenshan Botanical Garden, Shanghai 201602, China
  • Online:2010-12-25 Published:2011-01-28
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By analysis of genetic diversity and population genetic structure in natural populations of Sinocalycanthus chinensis with intersimple sequence repeat(ISSR) technique, the sampling strategy were determined based on the gene capture curve of 95% allele captured. The results are as follows: 6 populations to be sampled, the sampling proportion should be 30% and the distance between the sampled individuals are best of 26 m above. After seeds sampling and conduction of Exsitu conservation, the genetic diversity of the offspring population were evaluated that it conserved the genetic diversity of the natural populations effectively. Therefore, the systemic sampling method of endangered plants for Exsitu conservation of genetic diversity were discussed.

Key words: Sinocalycanthus chinensis, Genetic diversity, Exsitu conservation, Sampling strategy

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