Plant Diversity ›› 2012, Vol. 34 ›› Issue (4): 383-.DOI: 10.3724/SP.J.1143.2012.12005

• Articles • Previous Articles     Next Articles

Profiling of Membrane Lipids of Arabidopsis Roots during Catechin Treatment

 ZHENG  Guo-Wei-1, CHEN  Jia-2, LI  Wei-Qi-1   

  1. 1 The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    2 Yunnan Reascend Tobacco Technology (Group) CO., LTD., Kunming 650106, China
  • Received:2012-01-12 Online:2012-08-25 Published:2012-02-15
  • Supported by:

    Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China (0807B01211), West Light Foundation of the Chinese Academy Sciences


Catechin is a kind of phytotoxin and can kill plant cells in an hour. It can be developed as herbicide to kill weeds due to its strong phytotoxic activity. The main effect of this chemical is to trigger the death of the root system. To understand the response of root cell membrane lipids to catechin stress, we used the lipidomics approach to study the profiles of Arabidopsis root lipids molecules under catechin treatment. The changes of molecular species in membrane lipids, content of membrane lipids, double bond index (DBI) and acyl chain carbon number of the fatty acid were examined in Wild type (WS) and PLDδ deficient mutant (PLDδKO) during catechin treatment. The results indicated that after 90 min treatment with catechin, the lipid contents of digalactosyldiacylglycerol (DGDG), monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), phosphatidylcholine (PC), and phosphatidylinositol (PI) decreased both in WS and PLDδKO roots, lipid contents of phosphatidylethanolamine (PE) and phosphatidylinositol (PS) decreased in WS roots, but increase in PLDδKO roots, lipid contents of (phosphatidic acid) PA increased at the begin of treatment and declined to the level of control in WS roots. The ratio of the two major lipids in roots, PC and PE, declined significantly in PLDδKO plants, the acyl carbon number of PS in WS plants increased. The results suggested that PLDδKO is more sensitive than WS during catechin treatment, and suppression of PLDδ exacerbated membrane damage induced by catechin.

Key words: Phytotoxin, Lipidomics, Catechin, Arabidopsis

CLC Number: