Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distyloushomostylous species, using MiSeq sequencing

Plant Diversity ›› 2016, Vol. 38 ›› Issue (02): 110-113.

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Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distyloushomostylous species, using MiSeq sequencing

Wei Zhou1, Haidong Li2,3, Zhikun Wu4, Spencer C.H. Barrett5, Dezhu Li 1, Hong Wang2*

1. Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
2. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
3. Kunming College of Life Sciences, University of Chinese Academy of Sciences, Kunming 650201, China
4. Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
5. Department of Ecology and Evolutionary Biology, University of Toronto, Toronto Ontario M5S 3B2, Canada

Received:2015-12-05 Revised:2015-12-24 Online:2016-04-25 Published:2016-04-25
Supported by:
National Key Basic Research Program of China (2014CB954100), the Key Research Program of the Chinese Academic of Sciences (KJZD-EW-L07), the National Natural Science Foundation of China (31200289, 31570384), and the Natural Science Foundation of Yunnan Province (2012FB182)

Abstract

Abstract:

Primula chungensis is a species with considerable floral and mating-system variation, including distylous (outcrossing), homostylous (selfing) and mixed populations that contain both outcrossing and selfing forms. We isolated 24 microsatellite markers from P.chungensis using Illumina MiSeq sequencing. Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet. All loci were polymorphic with the number of alleles per locus ranging from 2 to4. The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708, respectively. The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P.chungensis and will inform models of the evolutionary history of mating systems in the species.

Key words: Distyly, Homostyly, Microsatellites, Polymorphism, Primula chungensis

Wei Zhou, Haidong Li, Zhikun Wu, Spencer C.H. Barrett, Dezhu Li , Hong Wa. Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distyloushomostylous species, using MiSeq sequencing[J]. Plant Diversity, 2016, 38(02): 110-113.

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Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distyloushomostylous species, using MiSeq sequencing

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