Abstract: The information of protein subcellular localization is important to our understanding of a protein function . The protein encoded by At4g22890 was predicted to be an Arabidopsis chloroplast protein . The 5′208 bp fragment of this gene was cloned and fused with GFP to construct a binary vector pMON530-cTP-GFP for genetic transformation . On confocal laser-scanning microscopy, green fluorescent signals were localized in chloroplasts in transgenic Arabidopsis plants, suggesting that the 208 bp DNA fragment encodes a peptide targeting to chloroplast . The results suggest that At4g22890 encodes a chloroplast protein .

Key words: Arabidopsis thaliana