Plant Diversity ›› 2012, Vol. 34 ›› Issue (4): 403-.doi: 10.3724/SP.J.1143.2012.11179

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Modification of pFGC5941 Construct and Transfer of Double Antisense Genes of NAC1 and SIP1 into Arabidopsis thaliana

LIANG  Peng-1, KONG  Xiang-Xiang-2, WANG  Chun-Tao-2, ZHA  Xiang-Dong-1, HU  Xiang-Yang-2   

  1. 1 Institute of Life Sciences, Anhui University, Hefei 230031, China; 2 Kunming Institute of Botany,
    Chinese Academy of Sciences, Kunming 650201, China
  • Received:2011-12-28 Online:2012-08-25 Published:2012-04-19
  • Supported by:

    The Major Science and Technology Program (110201101003TS03, 2011YN02和2011YN03)

Abstract:

SIP1 encodes the protein which show interact with SOS2, the protein involving in plant responding to saline stress while NAC1 encodes the protein which involves in auxin signal and promoting the development of lateral root of Arabidopsis thaliana. In present study SIP1 and NAC1-sense and NAC1-anti were inserted into pFGC5941S constructs, making the expression vectors pFGC5941S-SIP1-NAC1-sense and pFGC5941S-SIP1-NAC1-anti, respectively. Fifteen transgenic A.thaliana harboring these two constructs (pFGC5941S-SIP1-NAC1-sense and pFGC5941S-SIP1-NAC1-anti) were then generated via Agrobacterium tumefaciens-mediated transformation. The phenotypes of homozygous transformants which were grown on MS medium with 75mmol·L-1 NaCl showed that compared with wild-type A.thaliana, pFGC5941S-SIP1-NAC1-sense transgenic plants exhibited longer main root and increasing amounts of lateral roots, while no obvious differences were observed in pFGC5941S-SIP1-NAC1-anti transgenic plants. These results indicated that the development of A.thaliana lateral roots under salt stress was specifically promoted by both overexpression of SIP1 gene and NAC1 gene.

Key words: NAC1 gene, SIP1 gene, Antisense-expession vector, Double genes-expession vector, Arabidopsis thaliana transformation, Phenotypes

CLC Number: 

  • Q 75