Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 683-689.DOI: 10.3724/SP.J.1143.2011.11051

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Application of HAT-RAPD Technique in Identifying Natural Hybrids of Roscoea (Zingiberaceae)

 DU  Guang-Hui-1、2, LI  Qing-Jun-1   

  1. 1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
    2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-03-23 Online:2011-12-25 Published:2011-04-19


 Natural hybridization is very common in plants, and plays an important role in plant evolution. Besides the traditional methods including morphological analysis and hand crossing, molecular evidence is needed for studying natural hybridization.In order to analyze natural hybridization in Roscoea,HAT-RAPD technique was used to identify putative hybrids from parental species by principal coordinate analysis and hybrid index. The results indicated that the bands amplified by HAT-RAPD technique were more stable and reliable than that of RAPD. The result of principal coordinate analysis and hybrid index showed that intermediate individuals were the hybrids of R.humeana and R.cautleoides, and showed closer relationships to R.humeana. These results suggested that HAT-RAPD could be used to study natural hybridization. As it is simple and easy to manipulate, HATRAPD may prove to be a very effective technique in hybrid identification in the studies of plant evolution.

Key words: Roscoea humeana, R.cautleoides, Putative hybrids, HAT-RAPD, PCOA, Hybrid index

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