Plant Diversity ›› 2012, Vol. 34 ›› Issue (01): 56-62.doi: 10.3724/SP.J.1143.2012.11057

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Cloning and Analysis of NBSLRR Type Disease Resistance Gene Analogs from Rosa rubus in Yunnan

CHEN  Ling-1、2, ZHANG  Hao-1, QIU  Xian-Qin-1, YAN  Hui-Jun-1, WANG  Ji-Gang-1, JIAN  Hong-Ying-1, TANG  Kai-Xue-1   

  1. 1 Flower Research InstitutFlower Breeding Key Labe, Yunnan of Agricultural Sciences, Kunming 650205, China;
    2 College of Life Sciences, Yunnan University, Kunming 650091, China
  • Received:2011-04-02 Online:2012-02-25 Published:2011-09-23

Abstract:

In the study of NBS resistance genes of Yunnan wild Rosa, the degenerate oligonucleotide primers were designed according to the conserved motifs NBSLRR of most plant resistance (R) genes. The cDNA fragments were isolated from Rosa rubus by reverse transcription PCR (RTPCR). Then four resistance gene analogs (RGAs) were sequenced and named as AC9, AC39, AC50 and AC68, which contain NBSLRR domain. The sequence of each RGAs was compared with NBS region of eleven resistance genes reported before, and the similarities of aminoacid sequences ranged from 5.4% to 79.2%, and these four RGAs were clustered a group with Mi、RPS2、Pib and RPM1 gene. These results showed that the four RGAs will be used as molecular markers for screening candidate disease resistance genes and genetic map construction of Rosa rubus in the future.

Key words: Rosa rubus, Conserved motif, Resistance gene analogs (RGAs), Degenerate oligonucleotide primers

CLC Number: 

  • Q 78