MS1521 Gene is Required for Anther Development  of Arabidopsis thaliana

doi:10.3724/SP.J.1143.2010.10083

Abstract

Abstract:

This paper described the functional analyses of the MS1521 gene in anther development by using three Arabidopsis male sterile mutants (ms1521, st350 and st454). Mutant ms1521 was generated by ethylmethane sulfonate (EMS) mutagenesis treatment. Genetic analysis showed that ms1521 mutant was controlled by a single recessive nuclear gene. A mapbased cloning approach was used, and MS1521 was mapped to a region of 26 kb on chromosome 1 which contained a floralorgan identity gene UFO. Sequence analysis revealed that ms1521 had an AACGAC basepair change in the UFO coding region, which resulted in the replacement of an Asn by an Asp residue in the Cterminal region. The other two mutants, st350 and st454 with same phenotypes of ms1521, were identified from a TDNA insertion mutant population. Direct sequence analyses showed that both mutants had a premature stop codon in the UFO coding region. Allelism tests indicated that ST350, ST454 and MS1521 belonged to the same locus. These three mutants grew normally during the vegetative growth stage, but showed developmental defects at the reproductive growth stage: some stamens failed to develop anther, while others had normal anthers but their filaments were shorter than that of the wild type and others with normal stamens but failed in anther dehiscence. All the defects described above leaded to the sterile phenotype. Further cytological observations showed that the mutant anthers lacked of cell wall fiberization and secondary thickening in the endothecium which was required for anther dehiscence. These results indicated that MS1521 gene played an important role in Arabidopsis anther development.

Key words: Arabidopsis thaliana, Male sterile, Map-based cloning, Anther development, MS1521

CLC Number:

Q 944
Q 75

ZHOU Que, HUANG Xue-Yong, YANG Zhong-Nan, ZHANG Sen. MS1521 Gene is Required for Anther Development of Arabidopsis thaliana[J]. Plant Diversity, 2010, 32(6): 528-534.

References

Alexander MP, 1969. Differential staining of aborted and nonaborted pollen \
[J\]. Stain Technology, 44: 117—122
Bonner L, Dickinson H, 1989. Anther dehiscence in Lycopersicon esculentum Mill \
[J\]. New Phytologist, 113 (1): 97—115
Dawson J, Sozen E, Vizir I et al, 1999. Characterization and genetic mapping of a mutation (ms35) which prevents anther dehiscence in Arabidopsis thaliana by affecting secondary wall thickening in the endothecium \
[J\]. New Phytologist, 144 (2): 213—222
del Camplllo E, Lewls LN, 1992. Occurrence of the 95 cellulase and other hydrolases in flower reproductive organs undergoing major cell wall disruption \
[J\]. Plant Physiology, 99 (3): 1015—1020
Durfee T, Roe JL, Sessions RA et al, 2003. The Fboxcontaining protein UFO and AGAMOUS participate in antagonistic pathways governing early petal development in Arabidopsis \
[J\]. Proceedings of the National Academy of Sciences, 100 (14): 8571—8576
Feys B, Benedetti CE, Penfold CN et al, 1994. Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen \
[J\]. The Plant Cell, 6 (5): 751—759
Goldberg RB, Beals TP, San ders PM, 1993. Anther development：basic principles and practical applications \
[J\]. The Plant Cell, 5 (10): 1217—1229
Hepworth SR, Klenz JE, Haughn GW et al, 2006. UFO in the Arabidopsis inflorescence apex is required for floralmeristem identity and bract suppression \
[J\]. Planta, 223 (4): 769—778
Ishiguro S, KawaiOda A, Ueda J et al, 2001. The DEFECTIVE IN ANTHER DEHISCIENCE gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in Arabidopsis \
[J\]. The Plant Cell, 13 (10): 2191—2209
Keijzer C, 1987. The processes of anther dehiscence and pollen dispersal. I. The opening mechanism of longitudinally dehiscing anthers \
[J\]. New Phytologist, 105 (3): 487—489
Park JH, Halitschke R, Kim HB et al, 2002. A knockout mutation in allene oxide synthase results in male sterility and defective wound signal transduction in Arabidopsis due to a block in jasmonic acid biosynthesis \
[J\]. The Plant Journal, 31 (1): 1—12
Patton EE, Willems AR, Tyers M, 1998. Combinatorial control in ubiquitindependent proteolysis: don′t skip the Fbox hypothesis \
[J\]. Trends in Genetics, 14 (12): 236—243
Samach A, Klenz JE, Kohalmi SE et al, 1999. The UNUSUAL FLORAL ORGANS gene of Arabidopsis thaliana is an Fbox protein required for normal patterning and growth in the floral meristem \
[J\]. The Plant Journal, 20 (4): 433—445
Sanders PM, Bui AQ, Goldberg RB, 1999. Anther developmental defects in Arabidopsis thaliana malesterile mutants \
[J\]. Sexual Plant Reproduction, 11 (6): 297—322
Sanders PM, Lee PY, Biesgen C et al, 2000. The Arabidopsis DELAYED DEHISCENCE1 gene encodes an enzyme in the jasmonic acid synthesis pathway \
[J\]. The Plant Cell, 12 (7): 1041—1061
SteinerLange S, Unte US, Ecksrein L et al, 2003. Disruption of Arabidopsis thaliana MYB26 results in male sterility due to nondehiscent anther \
[J\]. The Plant Journal, 34 (4): 519—528
Stintzi A, Browse J, 2000. The Arabidopsis malesterile mutant, opr3, lacks the 12oxophytodienoic acid reductase required for jasmonate synthesis \
[J\]. Proceedings of the National Academy of Sciences, 97 (19): 10625—10630
von Malek B, van der Graaff E, Schneitz K et al, 2002. The Arabidopsis malesterile mutant dde22 is defective in the ALLENE OXIDE SYNTHASE gene encoding one of the key enzymes of the jasmonic acid biosynthesis pathway \
[J\]. Planta, 216 (1): 187—192
Wang X, Feng S, Nakayama N et al, 2003. The COP9 signalosome interacts with SCFUFO and participates in Arabidopsis flower development \
[J\]. The Plant Cell, 15 (5):1071—1082
Xie DX, Feys BF, James S et al, 1998. COI1: An Arabidopsis gene required for jasmonateregulated defense and fertility \
[J\]. Science, 280: 1091—1094
Xin ZG, Velten JP, Oliver MJ et al, 2003. Highthroughput DNA extraction method suitable for PCR \
[J\]. Biotechniques, 34: 1—6
Yang CY, Xu ZY, Song J et al, 2007. Arabidopsis MYB26/MALE STERILE35 regulates secondary thickening in the endothecium and is essential for anther dehiscence \
[J\]. The Plant Cell, 19 (2): 534—548
Yi J (易君), Gao JF (高菊芳), Zhang ZB (张在宝) et al, 2006. Genetic and mapping analysis of Arabidopsis thaliana male sterile mutant ms1502 (Cruciferae) \
[J\].Acta Botanica Yunnanica (云南植物研究), 28 (3): 283—288
Sun Y, Zhang W, Li FL et al, 2000. Identification and genetic mapping of four novel genes that regulate leaf development in Arabidopsis \
[J\]. Cell Research, 10 (4): 325—335
Zhang ZB, Zhu J, Gao JF et al, 2007. Transcription fasctor AtMYB103 in required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis \
[J\]. Plant Journal, 52 (3): 528—538
Zhou Q (周鹊), Jia QS (贾琦石), Yang ZN (杨仲南) et al, 2008. Genetic mapping of an Arabidopsis thaliana male sterile gene MS1521 \
[J\]. Journal of Shanghai Normal University (Natural Sciences), 37 (3): 296—300

[1]	Xiao Zhang, Guimei Chang, Zihao Wu, Jinpeng Wan, Jun Yang, Feijun Wang, Fang Wang, Diqiu Yu, Peng Xu. Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X [J]. Plant Diversity, 2022, 44(02): 213-221.
[2]	Fei-Hong Yan, Li-Ping Zhang, Fang Cheng, Dong-Mei Yu, Jin-Yong Hu. Accession-specific flowering time variation in response to nitrate fluctuation in Arabidopsis thaliana [J]. Plant Diversity, 2021, 43(01): 78-85.
[3]	Mulan Wang a, b, , Yunmei Shen c, , Faqing Tao b, Shengchao Yang a, Weiqi Li b. Submergence induced changes of molecular species in membrane lipids in Arabidopsis thaliana [J]. Plant Diversity, 2016, 38(03): 156-162.
[4]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Changes of Membrane Stability in PotassiumStressed Plants [J]. Plant Diversity, 2014, 36(05): 595-602.
[5]	JIA Yan-Xia, TAO Fa-Qing, LI Wei-Qi. Analysis of Endogenous Hormone Levels to Reveal the Retardation by Suppression of Phospholipase Dα1 in Arabidopsis Leaves during Hormonepromoted Senescence [J]. Plant Diversity, 2014, 36(04): 485-496.
[6]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Plants Adapt to LongTerm Potassium Deficiency by Accumulation of Membrane Lipids in Leaves and Maintenance of Lipid Composition in Roots [J]. Plant Diversity, 2014, 36(02): 163-176.
[7]	CHENG Zhi-Peng, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. ST273 Is Essential for Tapetum and Microspore Development in Arabidopsis thaliana* [J]. Plant Diversity, 2012, 34(5): 502-508.
[8]	LIN Yuan-Yuan- , YU Di-Qiu. Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments [J]. Plant Diversity, 2012, 34(5): 509-518.
[9]	LIANG Peng-, KONG Xiang-Xiang-, WANG Chun-Tao-, ZHA Xiang-Dong-, HU Xiang-Yang. Modification of pFGC5941 Construct and Transfer of Double Antisense Genes of NAC1 and SIP1 into Arabidopsis thaliana [J]. Plant Diversity, 2012, 34(4): 403-.
[10]	ZHAO Juan, CHEN Yan, HUI Sheng-Ming, TU Wei-Yi, CHEN Chen, GAO Ju-Fang. Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation [J]. Plant Diversity, 2012, 34(2): 164-170.
[11]	LI Wan-Sha, WANG Chun-Tao, HU Xiang-Yang. Isolation and Identification of an Arabidopsis thaliana Mutant with Serration Leaf Margin [J]. Plant Diversity, 2012, 34(01): 28-32.
[12]	GUO Thao-, LI Wan-Sha-, ZHA Xiang-Dong. [J]. Plant Diversity, 2011, 33(6): 645-652.
[13]	NIU Xue-Yan-, ZHANG Hai-Jun-, BAI Xue-Gui-, LI Wan-Sha-, LIU Xiao-Guang-, HU Xiang-Yang. Proteomics Reveal the Molecular Mechanism of Arabidopsis thaliana on Bacterial Signal N-3-oxo-hexanoyl-homoserine-lactone (OHHL) [J]. Plant Diversity, 2011, 33(4): 389-395.
[14]	ZHAO Xian-Ling, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. MS1516 is Essential for Tetrad Formation and Microspore Development in Arabidopsis thaliana [J]. Plant Diversity, 2011, 33(4): 383-388.
[15]	YANG Hui- , GAO Bo-, LI Wei-Qi. Lipid Peroxidation Induced by Metal Ion Stress is Positively Related to Lonic Polarization in Plants [J]. Plant Diversity, 2011, 33(3): 293-298.

MS1521 Gene is Required for Anther Development of Arabidopsis thaliana

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics