Profiling of Membrane Lipids of Arabidopsis Roots during Catechin Treatment

doi:10.3724/SP.J.1143.2012.12005

Abstract

Abstract:

Catechin is a kind of phytotoxin and can kill plant cells in an hour. It can be developed as herbicide to kill weeds due to its strong phytotoxic activity. The main effect of this chemical is to trigger the death of the root system. To understand the response of root cell membrane lipids to catechin stress, we used the lipidomics approach to study the profiles of Arabidopsis root lipids molecules under catechin treatment. The changes of molecular species in membrane lipids, content of membrane lipids, double bond index (DBI) and acyl chain carbon number of the fatty acid were examined in Wild type (WS) and PLDδ deficient mutant (PLDδKO) during catechin treatment. The results indicated that after 90 min treatment with catechin, the lipid contents of digalactosyldiacylglycerol (DGDG), monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), phosphatidylcholine (PC), and phosphatidylinositol (PI) decreased both in WS and PLDδKO roots, lipid contents of phosphatidylethanolamine (PE) and phosphatidylinositol (PS) decreased in WS roots, but increase in PLDδKO roots, lipid contents of (phosphatidic acid) PA increased at the begin of treatment and declined to the level of control in WS roots. The ratio of the two major lipids in roots, PC and PE, declined significantly in PLDδKO plants, the acyl carbon number of PS in WS plants increased. The results suggested that PLDδKO is more sensitive than WS during catechin treatment, and suppression of PLDδ exacerbated membrane damage induced by catechin.

Key words: Phytotoxin, Lipidomics, Catechin, Arabidopsis

CLC Number:

Q 945

ZHENG Guo-Wei-, CHEN Jia-, LI Wei-Qi. Profiling of Membrane Lipids of Arabidopsis Roots during Catechin Treatment[J]. Plant Diversity, 2012, 34(4): 383-.

References

Bais HP, Walker TS, Kennan AJ et al., 2003. Structuredependent phytotoxicity of catechins and other flavonoids: flavonoid conversions by cellfree protein extracts of Centaurea maculosa (spotted knapweed) roots［J］. Journal of Agricultural and Food Chemistry, 51 (4): 897—901
Cullis PR, Hope MJ, 1978. Effects of fusogenic agent on membrane structure of erythrocyte ghosts and the mechanism of membrane fusion［J］. Nature, 271 (5646): 672—674
D′Abrosca BD, Dellagreca M, Fiorentino A et al., 2006. Chemical constituents of the aquatic plant Schoenoplectus lacustris: Evaluation of phytotoxic effects on the green alga Selenastrum capricornutum［J］. Journa of Chemical Ecology, 32 (1): 81—96
Devaiah SP, Roth MR, Baughman E et al., 2006. Quantitative profiling of polar glycerolipid species from organs of wildtype Arabidopsis and a PHOSPHOLIPASE Dα1 knockout mutant［J］. Phytochemistry, 67 (17): 1907—1924
Hazei JR, Williams EE, 1990. The role of alterations in membrane lipid compositon in enabling physiological adaptation of organisms to their physical environment［J］. Progress in Lipid Research, 29 (3): 167—227
Hong YY, Zhang WH, Wang XM, 2010. Phospholipase D and phosphatidic acid signalling in plant response to drought and salinity［J］. Plant Cell and Environment, 33 (4): 627—635
Inderjit, Pollock JL, Callaway RM et al., 2008. Phytotoxic effects of (±)catechin in vitro, in soil, and in the field［J］. PLoS ONE, 3(7): e2536
Lee JS, Kim HJ, Park H et al., 2002. New diarylheptanoids from the stems of Carpinus cordata［J］. Journal of Natural Products, 65 (9): 1367—1370
Li WQ, Li MY, Zhang WH et al., 2004. The plasma membranebound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana［J］. Nature Biotechnology, 22 (4): 427—422
Li WQ, Wang R, Li MY et al., 2008. Differential degradation of extraplastidic and plastidic lipids during freezing and postfreezing recovery in Arabidopsis thaliana［J］. Journal of Biological Chemistry, 283 (1): 461—468
Li Y (李艳), Tian B (田波), Li WQ (李唯奇), 2011. Suppression of phospholipase Dδ enhances the membrane damage induced by UVB irradiation［J］. Plant Diversity and Resources (植物分类与资源学报), 33 (3): 299—305
Lu NA, Chen PQ, Yang Q et al., 2011. Anti and prooxidant effects of (+)catechin on hemoglobininduced protein oxidative damage［J］. Toxicology in Vitro, 25 (4): 833—838
Ma J, Luo XD, Protiva P et al., 2003. Bioactive novel polyphenols from the fruit of Manilkara zapota (Sapodilla) ［J］. Journal of Natural Products, 66 (7): 983—986
Meyer AS, Heinonen M, Frankel EN, 1998. Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin, and ellagic acid on human LDL oxidation［J］. Food Chemistry, 61 (12): 71—75
Munnik T, 2001. Phosphatidic acid: an emerging plant lipid second messenger［J］. Trends in Plant Science, 6 (5): 227—233
Murashige T, Skoog F, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures［J］. Physiologia Plantarum, 15 (3): 473—497
Ono K, Nakao M, Toyota M et al., 1998. Catechin production in cultured Polygonum hydropiper cells［J］. Phytochemistry, 49 (7): 1935—1939
Paszkowski WL, Kremer RJ, 1988. Biological activity and tentative identificatioin of flavonoid components in velvetleaf (Abutilon theophrasti Medik.) seed coats［J］. Journal of Chemical Ecology, 14(7): 1573—1582
Simes K, Du J, Kretzschmar FS et al., 2008. Phytotoxic catechin leached by seeds of the tropical weed Sesbania virgata［J］. Journa of Chemical Ecology,34 (5): 681—687
Su BN, Cuendet M, Hawthorne ME et al., 2002. Constituents of the bark and twigs of Artocarpus dadah with cyclooxygenase inhibitory activity［J］. Journal of Natural Products, 65 (2): 163—169
Testerink C, Munnik T, 2005. Phosphatidic acid: a multifunctional stress signaling lipid in plants［J］. Trends in Plant Science, 10 (8): 368—375
Tocquin P, Corbesier L, Havelange A et al., 2003. A novel high efficiency, low maintenance, hydroponic system for synchronous growth and flowering of Arabidopsis thaliana［J］. BMC Plant Biology, 3 (1): 2
Vuong QV, Stathopoulos CE, Nguyen MH et al., 2011. Isolation of green tea catechins and their utilization in the food industry［J］. Food Reviews International, 27 (3): 227—247
Wang XM, 2004. Lipid signaling［J］. Current Opinion in Plant Biology, 7 (3): 329—336
Wang XM, 2005a. Phospholipase D in hormonal and stress signaling［J］. Current Opinion in Plant Biology, 5 (5): 408—414
Wang XM, 2005b. Regulatory functions of phospholipase D and phosphatidic acid in plant growth, development, and stress responses［J］. Plant Physiology, 139 (2): 566—573
Wang XM, Devaiah SP, Zhang WH et al., 2006. Signaling functions of phosphatidic acid［J］. Progress in Lipid Research, 45 (3): 250—278
Weir TL, Bais HP, Stull VJ et al., 2006. Oxalate contributes to the resistance of Gaillardia grandiflora and Lupinus sericeus to a phytotoxin produced by Centaurea maculosa［J］. Planta, 223 (4): 785—795
Welti R, Li WQ, Li MY et al., 2002. Profiling membrane lipids in plant stress responses: Role of phospholipase dalpha in freezinginduced lipid changes in Arabidopsis［J］. Journal of Biological Chemistry, 277 (35): 31994—32002
Yang H, Protiva P, Cui BL et al., 2003. New bioactive polyphenols from Theobroma grandiflorum (“Cupuacu”) ［J］. Journal of Nautral Product, 66 (11): 1501—1504
Yeagle PL, Hutton WC, Huang CH et al., 1976. Structure in the polar head region of phospholipid bilayers: A 31P {1H} nuclear overhauser effect study［J］. Biochemistry, 15 (10): 2121—2124
Zhang WH, Wang CX, Qin CX et al., 2003. The oleatestimulated phospholipase D, PLDδ, and phosphatidic acid decrease H2O2induced cell death in Arabidopsis［J］. The Plant Cell, 15 (10): 2285—2295
Zheng GW, Tian B, Zhang FJ et al., 2011. Plant adaptation to frequent alterations between high and low temperatures: remodelling of membrane lipids and maintenance of unsaturation levels［J］. Plant Cell and Environment, 34 (9): 1431—1442

[1]	Fei-Hong Yan, Li-Ping Zhang, Fang Cheng, Dong-Mei Yu, Jin-Yong Hu. Accession-specific flowering time variation in response to nitrate fluctuation in Arabidopsis thaliana [J]. Plant Diversity, 2021, 43(01): 78-85.
[2]	Mulan Wang a, b, , Yunmei Shen c, , Faqing Tao b, Shengchao Yang a, Weiqi Li b. Submergence induced changes of molecular species in membrane lipids in Arabidopsis thaliana [J]. Plant Diversity, 2016, 38(03): 156-162.
[3]	LI Ai-Hua-, LING Li-Zhen-, LI Wei-Qi. Phospholipase Dδ is Involved in WoundingInduced Phosphatidic Acid Formation in Arabidopsis [J]. Plant Diversity, 2015, 37(4): 428-438.
[4]	HAN Xiao-, WANG Hou-Ping-, BO Jin-Jing-, HU Yan-Ru-, CHEN Xiao-Lan-**, YU Di-Qiu. Arabidopsis WRKY8 Transcription FactorAssociated Genes VQ10 and VQ11 are Responsive to Multiple Abiotic Stresses [J]. Plant Diversity, 2015, 37(06): 760-766.
[5]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Changes of Membrane Stability in PotassiumStressed Plants [J]. Plant Diversity, 2014, 36(05): 595-602.
[6]	JIA Yan-Xia, TAO Fa-Qing, LI Wei-Qi. Analysis of Endogenous Hormone Levels to Reveal the Retardation by Suppression of Phospholipase Dα1 in Arabidopsis Leaves during Hormonepromoted Senescence [J]. Plant Diversity, 2014, 36(04): 485-496.
[7]	WANG Dan-Dan, ZHENG Guo-Wei, LI Wei-Qi. Plants Adapt to LongTerm Potassium Deficiency by Accumulation of Membrane Lipids in Leaves and Maintenance of Lipid Composition in Roots [J]. Plant Diversity, 2014, 36(02): 163-176.
[8]	YU Xiao-Mei, WANG Rong, LI Wei-Qi. Changes in Membrane Lipids during Silique Senescence in Arabidopsis [J]. Plant Diversity, 2014, 36(02): 177-186.
[9]	JIA Yan-Xia-, LI Wei-Qi. Changes in Lysophospholipid and Degree of Unsaturated Membrane Lipids are Associated With Senescence in Arabidopsis Leaves [J]. Plant Diversity, 2013, 35(5): 569-577.
[10]	CHENG Zhi-Peng, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. ST273 Is Essential for Tapetum and Microspore Development in Arabidopsis thaliana* [J]. Plant Diversity, 2012, 34(5): 502-508.
[11]	LIN Yuan-Yuan- , YU Di-Qiu. Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments [J]. Plant Diversity, 2012, 34(5): 509-518.
[12]	YAN Xian-Lun-, WANG Chun-Tao- , KONG Xiang-Xiang-, YANG Yong-Ping-, HU Xiang-Yang. Simplification of Entry Vector by TA Approach [J]. Plant Diversity, 2012, 34(4): 397-.
[13]	LIANG Peng-, KONG Xiang-Xiang-, WANG Chun-Tao-, ZHA Xiang-Dong-, HU Xiang-Yang. Modification of pFGC5941 Construct and Transfer of Double Antisense Genes of NAC1 and SIP1 into Arabidopsis thaliana [J]. Plant Diversity, 2012, 34(4): 403-.
[14]	ZHAO Juan, CHEN Yan, HUI Sheng-Ming, TU Wei-Yi, CHEN Chen, GAO Ju-Fang. Genetic and Mapping Analysis of Arabidopsis thaliana Male Sterile Mutant filament no elongation [J]. Plant Diversity, 2012, 34(2): 164-170.
[15]	LI Wan-Sha, WANG Chun-Tao, HU Xiang-Yang. Isolation and Identification of an Arabidopsis thaliana Mutant with Serration Leaf Margin [J]. Plant Diversity, 2012, 34(01): 28-32.