Genetic Diversity Analysis of Nelumbo Accessions Based on ESTSSR Markers

doi:10.7677/ynzwyj201515004

Abstract

Abstract:

EST-SSR markers were applied to estimate the genetic diversity for 30 accessions of Nelumbo nucifera, 6 accessions of Nelumbo lutea and 14 hybrids between these two species. The 52 of 123 ESTSSR markers (423%) were screened and then applied to amplify the 50 Nelumbo accessions. A total of 177 alleles were identified, and the number of alleles per locus and Polymorphic Information Content (PIC) value varied from 2 to 8 with an average of 34 alleles and from 063 (NNFB1059) to 091 (NNFB750) with an average value of 079, respectively. Jaccard similarity coefficients of the amplification results were analyzed by NTSYSpc2.11 software and the genetic similarity coefficient was from 024 to 086. The clustering dendrogram constructed by UPGMA method indicated that 50 accessions of Nelumbo could be divided into four major groups at the similarity coefficient of 037. Group I and group II included Nnucifera; group III included the majority of AsianAmerican lotus hybrids; and group IV included Nlutea. In addition, the AsianAmerican hybrids were closer to Nnucifera based on genetic relationship, which is consistent with the traditional classification result and the previous reports.

Key words: Nelumbo, EST-SSR, Genetic diversity, Genetic relationship

CLC Number:

Q 16

XU Yu-Xian, ZHANG Wei-Wei, MO Hai-Bo, LI Chun, CAO Jian-Guo, TIAN Dai-Ke. Genetic Diversity Analysis of Nelumbo Accessions Based on ESTSSR Markers[J]. Plant Diversity, 2015, 37(05): 595-604.

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